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Pittsburgh Center for Kidney Research

An NIH-sponsored George M. O'Brien Kidney Research Center

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Center Overview

Welcome to the Pittsburgh Center for Kidney Research, a George M. O’Brien Kidney Research Center. The Center is supported by a P30 grant from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), by the University of Pittsburgh School of Medicine and Department of Medicine, and by the Icahn School of Medicine at Mount Sinai. Our Center is designed to facilitate basic and translational research, as well as training and information transfer related to kidney cell biology, physiology, and pathobiology. The components of our Center include the following:

Investigators - The Pittsburgh Center for Kidney Research supports over 100 investigators from across the Nation that study all aspects of kidney and lower urinary tract biology, physiology, and pathobiology. Our investigators have expertise that ranges from mathematics, to computational biology, to physiology, to genetics, to cell biology.

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Biomedical Cores and Services - The Pittsburgh Center for Kidney Research supports four research cores to advance our understanding of kidney and lower urinary tract biology in normal and disease states: the Physiology Core, the Animal and Translational Core, the Kidney Imaging Core, and the Model Systems and Therapeutics Core. Each core provides cutting edge research tools and expert support. The Center also supports a Biostatistics Service through its Administrative Core.

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Education - The Center offers diverse educational opportunities including mini-sabbaticals, seminars, and research training for medical students, graduate students, and undergraduate students. The Center also supports a series of annual meetings that are focused on acute kidney injury, protein trafficking, the ubiquitin-proteosome system, and epithelial physiology and cell biology (held in Telluride, CO).

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Pilot Projects - Funds are available to support pilot studies in the general areas of kidney and lower urinary tract biology, physiology, and pathobiology. Applications from new investigators, from investigators that are new to the field of renal research, or from senior renal investigators working in a new area are considered for funding.

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Publications - Use of our core facilities has resulted in hundreds of publications to date. Our manuscripts are published in journals that include the American Journal of Physiology, the EMBO Journal, the Journal of Biological Chemistry, the Journal of the American Association of Nephrology, Molecular Biology of the Cell, and Proceedings of the National Academy of Sciences.

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Biomedical Cores, Biostatistics Service, and Center Administration

The O’Brien Kidney Research Center at the University of Pittsburgh and Mount Sinai School of Medicine seeks to do the following: enhance the efficiency and productivity of a large number of kidney-related research projects currently in progress; make possible the acquisition of data for new projects; promote collaborations among center investigators; and provide tools to facilitate translational research. These functions depend on the Center's Biomedical Cores, which provide physiological, cell biological, genetic, analytical, molecular biological, and drug discovery tools, as well as model organisms. All our cores have unique resources for the renal research community and are designed to serve as a national and international resource. The Administrative Core of the Center also supports a Biostatistics service, which is available to all Center investigators. Click on the icons below to learn more about each of the cores and services.

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  • Core A: Physiology Core

    Core A: Physiology Core

    The Physiology Core (Core A) provides an important regional, national and international resource for investigators who wish to assess expression, localization and functional characteristics of ion channels and transporters as well as their regulation by accessory proteins in single isolated mammalian (and other) nephron segments, epithelial cell lines and heterologous expression systems. The overall concept behind the design of the Core is to provide specialized technical expertise and resources to study transepithelial transport and associated regulatory pathways with progressive degrees of complexity from single molecules to model systems and native epithelia. The Physiology Core aims to offer an integrated approach including functional, biochemical and molecular strategies to address relevant questions proposed by users about transport proteins and associated regulatory pathways. It is expected that the data generated from the specialized techniques and tools provided through this Core will be complemented by studies performed in other Center Cores, including the Animal and Translational, Kidney Imaging, and Model Systems and Therapeutics Cores.

    Services provided by the Core:

    The Single Tubule and Microperfusion Subcore provides investigators with microdissected tubules for (i) quantitation of RNA expression and/or abundance by RT-PCR, quantitative PCR and microarray profiling, quantification of protein expression by immunoblotting, immunolocalization (in collaboration with the Kidney Imaging Core), and enzyme/transporter microassays, and performs (ii) fluorescence-based assays for analysis of channel/transporter function in individually identified cells in isolated mammalian (and other) tubules microperfused in vitro, and (iii) quantification of transepithelial ion/solute fluxes across isolated tubules microperfused in vitro.

    The Cellular Physiology Subcore provides methods for the study of transport proteins and associated regulatory proteins in heterologous expression systems, model epithelia and tissues. Electrophysiological techniques combined with manipulation of protein expression provide powerful tools to examine the function and regulation of epithelial transport proteins. This Subcore provides technologies for the analysis of surface expression and ion channel activity in Xenopus oocytes and mammalian expression systems of wild type and mutated ion channels with or without putative interacting or regulatory partners. This includes isolation and injection of oocytes with mRNAs coding for the proteins of interest, measurements of ion channel activity in oocytes with the two-electrode voltage clamp technique, measurements of whole-cell currents of mammalian cell and native epithelial cells with the patch-clamp technique, assessment of single channel properties and activity with the patch-clamp technique, and analysis and interpretation of electrophysiological experiments. Methods for the analysis of active transport, transepithelial resistance, tight-junction permeability and membrane capacitance of epithelial monolayers and native epithelia in Ussing chambers are also available through this Subcore.

    The Cell Biology Subcore provides methods for the analysis of expression, folding, maturation, post-translational modification and trafficking of transport proteins and associated regulatory proteins. This Subcore provides technologies for isolation of single cells, reverse transcription and second-strand complementary DNA (cDNA) synthesis, and quantification of transcript levels by qPCR and RNASeq. In addition, this Subcore provides well-established expertise in the study of post-translation modification of proteins, including phosphorylation, ubiquitination and palmitoylation.

    Training: The methods described above are well established in our laboratories and have been refined through constant use over the past couple decades. Sabbaticals are offered through the Center to investigators who wish to be instructed in isolation and microperfusion of single tubule from mouse, rat or rabbit kidneys. We provide training in isolation and injection of Xenopus oocyte, recording of currents from oocytes with the two-electrode voltage clamp technique, single channel and whole-cell patch-clamp recordings and Ussing chamber technologies. This Subcore also offers training to investigators who wish to examine expression, folding, maturation and post-translational modifications of transport proteins and associated regulatory pathways.

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    Request Core Use: Investigators interested in gaining access to any of our Core A facilities or educational opportunities are asked to contact Dr. Carattino or Dr. Satlin and provide us with the following information: (1) A short description of your proposed core use and/or the educational/training opportunity being requested; (2) information about your current and pending grant support.

    Core directors:

    Marcelo D. Carattino, PhD

    Lisa Satlin, MD

    The Single Tubule and Microperfusion Subcore Director: Lisa Satlin, MD

    The Cellular Physiology Subcore Directors: Thomas R. Kleyman, MD, Shaohu Sheng, MD, and Marcelo D. Carattino, PhD.

    Cell Biology Subcore Directors: Rebecca Hughey, PhD and Marcelo D. Carattino, PhD

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  • Core B: Animal and Translational Core

    Core B: Animal and Translational Core

    Overview of the goals, value-added services, and composition of the Animal and Translational Core. The explosive knowledge in the biochemical, genetic, and cellular basis of kidney disease and associated cardiovascular disorders provides unprecedented potential for decreasing the societal burden of these costly diseases. However, there are critical “bottlenecks” in the workflow such that essentially no progress has been made in the last 3 decades with regard to introducing new therapies to prevent or treat AKI or CKD. Two critical bottlenecks are: 1) the inability of many basic science labs to conduct rigorous preclinical studies in predictive animal models; and 2) the inability of both basic and preclinical labs to negotiate the barriers to human translation. The justification for and scientific premise of the Animal and Translational Core is to overcome these barriers by offering one-stop services for scientist who are conducting break-through kidney research, yet do not have the experience, resources, or personnel to shepherd their discoveries into the development pipeline (Figure 1).

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    The Animal and Translational Core has the wherewithal to measure, using robust techniques, whole kidney function and health. Because of the interrelationships between the kidney, autonomic nervous system, and cardiovascular system, it is insufficient to assess kidney function only. A more comprehensive approach is required. Therefore the Core offers not only techniques for assessing kidney function/outcomes, but provides in addition approaches to rigorously measure cardiovascular and autonomic function in intact animals. Finally, a key ingredient in developing new drugs to treat kidney disease is the assessment of the interrelationships among time, dose, and concentrations of drug in body compartments. This is not a trivial exercise; specialized analytical methods, knowledge, and skills are required. The Animal and Translational Core can provide these.

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    Analysis of Energy Pathway Molecules. Precise measurement of the energy status of renal cells is critical to understanding the underlying pathophysiology of kidney diseases and to assessing the benefits of potential therapies. Although there are commercial kits for ATP, often the quality of such kits is disappointing. Moreover, a complete analysis of energy status requires measurement of a large set of energy-related molecules.

    ATP carries three negative charges; therefore separating ATP by HPLC requires a mobile phase that gives sharp chromatographic peaks for highly charged molecules. Unfortunately, such mobile phases severely suppress ionization in the ion source of mass spectrometers. To circumvent this problem, we make the N6-etheno derivative of ATP (which is highly fluorescent) by reacting the sample with chloroacetaldehyde. Then we measure N6-ethenoATP by HPLC coupled to a fluorescence detector. By so doing, we increase the sensitivity of the assay approximately 3000-fold over UV detection. Moreover, the reaction also converts ADP, AMP, adenosine, NAD+, NADH, NADP+, and NADPH to their corresponding fluorescent N6-etheno derivatives. Therefore, we can simultaneously, accurately, and with exquisite sensitivity (<0.5 pmole) measure all of these energy related molecules. In addition to measuring these energy molecules, to complete the suite of energy-related assays, we also developed HPLC assays to measure mitochondrial-related molecules such as cardiolipin, citrate synthase, rotenone-sensitive NADH oxidase, and GC-MS assays for all of the intermediates in the Krebs cycle. Thus, we have an invaluable toolkit for studying energy-related issues in AKI, CKD, and other kidney diseases. Figure 2 shows an HPLC-fluorescence chromatogram for N6-ethenoATP, N6-ethenoADP, N6-ethenoAdenosine, and N6-ethenoNECA (internal standard). Figure 2 also shows standard curves for N6-ethenoATP, N6-ethenoADP, N6-ethenoAMP, and N6-ethenoAdenosine.

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    Request Core Use: Investigators interested in gaining access to any of our Core B facilities or educational opportunities are asked to contact Dr. Jackson or Dr. Stocker and provide us with the following information: (1) A short description of your proposed core use and/or the educational/training opportunity being requested; (2) information about your current and pending grant support.

  • Core C: Kidney Imaging Core

    Core C: Kidney Imaging Core

    The Kidney Imaging Core provides a national resource for Center users who require detailed and quantitative morphological analysis of kidney- and lower urinary tract-associated cells and tissues, particularly those that are epithelial in nature, as well as whole organs. The Imaging Core complements each of the other Center cores by providing critical information about the amounts, localization, and dynamics of organelles, cells, molecules, and tissues in normal and disease states. Thus, the Imaging Core is an integral part of the Center’s mission to support multidisciplinary, basic and translational research in physiology, pathophysiology, and molecular biology of the kidney, as well as training and information transfer amongst Center users and cores.

    Services Provided by the Core:

    The Epithelial Imaging Subcore specializes in the imaging and analysis of cultured kidney- and bladder-derived epithelial cells, as well as kidney and lower urinary tract-associated tissues and tissue slices. Imaging capture modalities include brightfield, DIC, darkfield, confocal, as well as STED and STORM-based microscopy. For samples that show weak fluorescence, a video camera system is available with associated digital deconvolution software. Analysis includes colocalization and tracking in 4D, as well as 3D reconstruction of individual epithelial cells and tissues using Imaris, Metamorph, and Volocity imaging softwares. Ultrastructural analyses include scanning electron microscopy (SEM), transmission electron microscopy (TEM), including ultrathin immunoelectron microscopy, to examine the distribution and relationship of single or multiple antigens. In addition, the Core includes facilities to perform high-resolution freeze-fracture analysis.

    The Stereology and 3D Organ Reconstruction Subcore provides image analysis that includes, but is not limited to stereology, a set of unbiased and accurate tools to measure parameters such as volume, surface area, length, and number. In addition, this subcore, along with the Epithelial Imaging Subcore, will provide 3D reconstructions of cells, tissues, and organs using Imaris, Neuroclida, Metamorph, Volocity, and StereoImager software packages.

    The Histology/Pathology Subcore specializes in the preparation of kidney and lower urinary tract tissues for histology and electron microscopy, and subsequent analysis of pathological findings by our expert kidney/lower urinary tract pathologist.

    The Live-Cell/Tissue/Organ Imaging Subcore is dedicated to microscopy of living urinary tract cells/tissues/organs in normal and pathological states. Imaging is provided by a dedicated Leica SP8 confocal system, an Olympus Fluoview FV1000 two-photon microscope system as well as by the University of Pittsburgh Center for Biological Imaging (CBI), which has additional two-photon setups. In addition, the CBI provides specialized imaging tools and analysis including fluorescence-resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), high-speed Ca2+ imaging, and high-speed total internal reflection fluorescence microscopy (TIR-FM).

    The Center for Critical Care Nephrology Tissue Bank is an important resource that provides our users human kidney tissue for performing morphological and biochemical analyses.

    Validated antibody collection: the collection includes antibodies the define specific cell types and nephron segments in the kidneys and lower urinary tract. Studies of the kidney and lower urinary tract often require markers that define a particular cell type or region of the kidney. The core provides antibodies to our users that recognize specific kidney segments and cell types in the lower urinary tract.

    Training: We provide training in the processing, staining, and imaging of kidney and lower urinary tract cells and tissues as well as interpretation of morphological data. To enhance the likelihood of successful morphological studies, the Core will provide advice and instruction in the design of these experiments. Beyond providing technical expertise, a crucial function of the Imaging Core will be to train investigators in the proper fixation, processing, and imaging of kidney and lower urinary tract tissues as well as training on the specialized instruments available to core users.

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    Request Core Use: Investigators interested in gaining access to any of our Core C facilities or educational opportunities are asked to contact Dr. Apodaca and provide him with the following information: (1) A short description of your proposed core use and/or the educational/training opportunity being requested; (2) information about your current and pending grant support.

    Core Director: Gerard Apodaca, PhD

    Epithelial Imaging Subcore Director: Gerard Apodaca, PhD

    Histology/Pathology Subcore Director: Sheldon Bastacky, MD

    3D Reconstruction Subcore Director: Carl Bates, MD

    Live Cell/Tissue/Organ Microscopy Subcore Directors: Katy Baty, PhD, Martin Oberbarnscheidt, MD, PhD, and Fadi Lakkis, MD

    Center for Biological Imaging Director: Simon Watkins, PhD

    Center for Critical Care Nephrology Tissue Bank:
    David Emlet, PhD

    Core Managers: Wily Giovanni Ruiz; Dennis Clayton

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  • Core D: Model Systems and Therapeutics Core

    Core D: Model Systems and Therapeutics

    The “Model Systems and Therapeutics Core” employs two successful, genetically-tractable systems that have been used continually in this O’Brien center, the yeast S. cerevisiae and zebrafish, each with distinct advantages.  In addition, we have recently added a third system to the core, human kidney organoids.  These experimental systems will continue to be used to examine how specific renal proteins fold and mature in the secretory pathway, how specific cellular stresses impact kidney function, and how molecular probes can intercede in various disease states.  Indeed, our interest in acute kidney injury (AKI) arose from high throughput screens using these models and from the generation of new experimental tools that derived from collaborations with Core users.  Hypotheses arising from the unique attributes of the yeast, zebrafish, and organoid models and from the use of chemical modulators will be tested in rodent models with the help of other Cores in our Center.  In turn, experiments the proposed model systems provide rapid assessments of predictions that emerge from more complex systems.  The long-range objectives of the Core are to identify and determine the mechanism of action of factors that influence the biogenesis of proteins that regulate renal cell function and that modulate AKI. The Aims of the Core are to:
    1. Provide a resource for investigators who wish to use yeast and zebrafish to determine how disease-associated proteins and cellular stresses modulate kidney development, repair, and function. Hypotheses that underscore this Aim include: (1) Cellular factors that affect the biogenesis of renal proteins expressed in yeast will similarly affect the trafficking and stability of the same proteins in human epithelial cells; (2) Small molecules that impact the function of these factors provide novel therapeutic targets to decrease the burden of renal diseases linked to protein biogenesis; (3) The chemical modulation of cellular stress responses and repair—identified through efforts using yeast and zebrafish models—provide novel avenues to treat AKI.  All discoveries that arise from this aim will subsequently be translated into organoid and rodent models.
    2. Assist with design, synthesis, and characterization of compounds to evaluate efficacy and properties. The focus of this aim will be to identify novel, patentable compounds that are more potent than the lead, and exhibit improved properties appropriate for pre-clinical development.  To achieve this, we will: a) Guide design and synthesis of novel analogs and evaluate activity in our standard yeast and zebrafish assays; b) Evaluate novel compounds in secondary in vitro pharmaceutical profiling assays, and a human kidney organoid AKI model; and c) Determine the efficacy of candidates in mouse models of AKI using standard treatment regiments in both simple and more complex models of AKI.

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    Request Core Use: Investigators interested in gaining access to any of our Core D facilities or educational opportunities are asked to contact Dr. Brodsky or Dr. Hukriede and provide us with the following information: (1) A short description of your proposed core use and/or the educational/training opportunity being requested; (2) information about your current and pending grant support.

    Core Directors:

    Yeast Model System: Jeffrey Brodsky, PhD

    Zebrafish Model System: Neil Hukriede, PhD

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  • Biostatistics Service

    Biostatistics Service

    The Pittsburgh Center for Kidney Research also offers a Biostatistics Service for Center Users. For additional information please contact Dr. Thomas Kleyman or Dr. Ora Weisz.

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    Service Director:

    Thomas Kleyman, MD

  • Administrative Core

    Administrative Core

    The Center for Kidney Research is managed by the Administrative Core, which oversees the Biomedical Cores and Biostatistics services, promotes new research through Pilot projects, enhances training through its educational component, and coordinates the addition of new Center Investigators. Members of the Administrative Core include the following:

    Center Director: Thomas Kleyman, MD

    Center Associate Director: Ora Weisz, PhD

    Physiology Core Directors: Marcelo Carattino, PhD and Lisa Satlin, MD

    Animal and Translational Core Directors: Ed Jackson, PhD and Sean Stocker, PhD

    Kidney Imaging Core Director: Gerard Apodaca, PhD

    Model Systems and Therapeutics Directors: Jeff Brodsky, PhD and Neil Hukriede, PhD

    Executive Steering Committee: Thomas Kleyman, MD; Ora Weisz, PhD; Gerard Apodaca, PhD; Jeffrey Brodsky, PhD; Marcelo Carattino, PhD; Neil Hukriede, PhD; Edwin Jackson, PhD; and Lisa Satlin, MD

    External Review Committee: Douglas Eaton, PhD (Emory University); Lisa Guay-Woodford, MD (Children's National Medical Center); David Pearce, MD (University of California at San Francisco); Ambra Pozzi, PhD (Vanderbilt University); Youhau Liu, PhD and John Kellum, MD (University of Pittsburgh)

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    Request Access to Center's Cores or Educational Opportunities: Investigators interested in gaining access to any of our Center's Cores or educational opportunities are asked to to contact the relevant Core director (or the Center Director/Co-director) using the email links above, and provide us with the following information: (1) A short description of your proposed core use and/or the educational/training opportunity being requested; (2) information about your current and pending grant support.

    Center Director:

    Thomas Kleyman, MD

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    Core A: Physiology Core

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    Core B: Animal and Translational Core

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    Core C: Kidney Imaging Core

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    Core D: Model Systems and Therapeutics

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    Biostatistics Service

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    Administrative Core

Pilot Projects

Our Center supports Pilot and Feasibility projects that are either basic science or translational in nature. Pilot and Feasibility projects are funded for up to two years in duration. Calls for applications, when available, are listed below.

Next deadline for submission: TBA

Eligibility: Three general categories of investigators are considered for Pilot and Feasibility funding: (1) newly independent investigators without current or previous R01 or VA Merit Review support; (2) established investigators with limited previous kidney-related research; and (3) established renal investigators proposing innovative ideas that represent a clear departure from ongoing research directions.

Proposal: Applicants must provide a brief description of their proposed project in an NIH-like style including Specific Aims, Significance, Preliminary data, and Aims. The total length of the proposal is limited to three pages, not including references. In addition, the applicant must provide (1) a brief outline of how they meet the eligibility criteria outlined above, (2) an updated NIH biosketch (including other support), (3) a “working title” for their proposed project, and (4) a statement of which Core(s) they will employ in their studies.

Scored criteria: The following criteria will be considered in evaluating the merit of each proposal:
• Scientific merit
• Innovation
• Methodology
• Likelihood that the work will advance understanding of renal cell biology, physiology, or pathophysiology
• Background and experience of the investigator
• Adequacy of resources and environment to support the project
• Likelihood that the pilot study will lead to subsequent extramural, peer-reviewed funding
• Use of the Core facilities
• Likelihood that the project that will foster interactions with members of our Center

Contact: For additional information contact Dr. Kleyman or Dr. Weisz.

Currently funded projects:

Rod Tan, MD and Kang Kim, PhD (UPSOM)
Non-invasive assessment of renal micro-vasculature of Nrf2 modulated mice using new super-resolution ultrasound imaging

Manisha Jhamb, MD (UPSOM)
Comprehensive Exercise (COMEX) Pilot study: Optimizing Exercise Participation to Improve Patient-centered Outcomes and Physical Functioning in Hemodialysis Patients

Rannar Airik, PhD (UPSOM)
Nephronophthisis gene ANKS6 regulates glomerular development

Keith Mostov, MD, PhD (UCSF)
A novel approach to acute kidney injury

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The Pittsburgh Center for Kidney Research is dedicated to providing education, training, and enrichment opportunities. Mini-sabbaticals and other instructional opportunities are available to new and established investigators, post-doctoral fellows, medical students, graduate students, and undergraduate students with the goal of bringing new technologies into the laboratories of investigators to broaden and enhance their research activities. The Center also supports summer research experiences for medical students and undergraduate students.

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  • Mini Sabbaticals

    Each Core offers 1 to 2 week “mini-sabbaticals”, where attendees work in one of the Cores to learn specific techniques. For example, our Physiology Core offers three to six month sabbaticals for scientists or trainees interested in acquiring the skills needed for successful microperfusion of isolated tubules for measurement of transport or functional fluorescence imaging. Our Center will assist investigators in arranging mini-sabbaticals, including housing arrangements.

    To participate in a mini-sabbatical, please download our core usage form and return by email to Dr. Thomas Kleyman or Dr. Ora Weisz.

  • Research Experience and Training

    Graduate Students: Faculty members associated with the Center have a long track-record of training graduate students in biomedical research. Prospective students interested in Ph.D. training are encouraged to apply to the following Ph.D. training programs: 

    The Interdisciplinary Biomedical Graduate Program (, with an emphasis in Cell Biology and Molecular Physiology ( 

    Integrative Systems Biology (

    Medical Students:
    Over eighty University of Pittsburgh medical students perform research during the summer between their first and second year. Dr. Kleyman is the PI of a T35 training grant (T35 DK065521) that provides support for medical students to perform research over the summer under the auspices of a faculty member. This support mechanism encourages students to perform research under the direction of one of our Center investigators. Students working in a kidney research laboratory participate in our renal division educational activities, which includes a series of lectures for our renal fellows that focuses on kidney physiology, pathophysiology, and disease management. They also attend a faculty-facilitated discussion on authorship issues, a practical talk about poster and platform presentations, writing abstracts, and ethical issues in research. The relevance of biomedical research is discussed, using a number of examples of molecular biologic discoveries and gene transfer in inherited immunodeficiency disorders, diabetes, and cystic fibrosis, to illustrate the importance of translating discoveries at the bench to the beside in order to improve clinical care and advance medicine.

    Undergraduate Students: For the past two years our Center has supported a summer research program for undergraduate students with funds provided by the NIDDK. Students are selected from the pool of applicants participating in the School of Medicine or the Department of Biological Sciences Summer Undergraduate Research Program (SURP). As part of their training, the School of Medicine SURP has weekly lunch sessions that focus on ethics, career pathways, lectures by selected faculty, and the admission process for graduate school and medical school. The Department of Biological Sciences SURP also has weekly lunch sessions that include faculty lectures, journal clubs, and discussion of career paths. In addition to these activities, students participating in our Kidney Center SURP meet weekly for a two-hour session with Center faculty. These sessions provide overviews of kidney physiology and pathophysiology, discussion of careers in kidney research and nephrology, and highlight areas of research within our Kidney Center. Sessions also review new publications in fields of research relevant to the kidney. The group also attends a day long ethics forum held at Duquesne University. At the end of the summer, all students prepare a written summary of their findings in abstract form and present their work to all SURP students and participating faculty.

  • Other Educational Opportunities

    Annual Acute Kidney Injury Retreat: Drs. Kleyman and Kellum host an annual daylong retreat to foster a cross-campus dialogue focused on AKI, with a goal of identifying common areas of interest and developing collaborations. University of Pittsburgh faculty and senior fellows present ongoing research and receive feedback from attendees. We invite outside experts in the area of AKI to participate in our retreat and present a seminar at the retreat, the day preceding the retreat at Critical Care Medicine grand rounds, and the day following the retreat at our Renal Division Grand Rounds. Invited speakers for the symposium held in 2012 included Lakhmir Chawla (George Washington University), Pierre Dagher (Indiana University) and Volker Hans Haase (Vanderbilt University). In addition to this yearly retreat, Dr. Kellum hosts a bimonthly meeting where investigators with an interest in AKI present data in an informal setting that encourages a dialog among participants.

    The Local Traffic Symposium: This one-day meeting, sixteen years in the running, explores the cell biology of membrane biogenesis and traffic. Participants include regional investigators, students, and postdocs from the University of Pittsburgh andCarnegie Mellon University as well as researchers and guest speakers from other universities in Pennsylvania, Ohio, and the NIH. The meeting includes student presentations, faculty talks, and a keynote address that provides a forum for students, faculty, and research support staff with an interest in membrane traffic to interact and share their recent findings on protein folding, ER degradation, secretory and endocytic traffic. Recent keynote speakers include James Rothman, Scott Emr, and Lois Weisman. Support for the meeting is provided by the Dean of the Medical School, the Department of Cell Biology, the Renal-Electrolyte Division, and the O’Brien Kidney Center. Attendance at this meeting typically exceeds 120 and is free for attendees.

    Ubiquitin-Proteosome Meeting: An annual day-long Pittsburgh Ubiquitin-Proteasome Meeting is organized by investigators from the Department of Biological Sciences (Brodsky), the School of Medicine/Hillman Cancer Center (Wan), and the Department of Pharmaceutical Sciences (Kwan) at the University of Pittsburgh. The meeting brings together the PIs and personnel from the ~10 groups in the Pittsburgh area, as well as researchers from other areas (e.g., Washington DC and West Virginia) whose work relates to various aspects of cellular protein quality control and the ubiquitin-proteasome pathway. The invited guest lecture for the 2012 meeting was Ron Kopito from Stanford University. Support is provided by the University of Pittsburgh School of Medicine.

    Annual Summer Workshop in Teluride: Dr. Kleyman has been co-organizing an annual week-long summer workshop on Epithelial Physiology and Cell Biology held in Telluride, Colorado, under the auspices of the Telluride Science Research Center for the past 10 years. The goal of this workshop is to bring together experts who present recent findings and generate discussions in the following areas: (i) structure and regulation of epithelial ion channels; (ii) structure and regulation of ion cotransporters, exchangers and pumps; and (iii) protein trafficking in epithelia. We encourage Core Directors/co-Directors, scientists supported by Pilot andFeasibility funding, as well as scientists utilizing our Core facilities to participate in this meeting. 


A major goal of our Center is to advance our knowledge of normal kidney function, cellular mechanisms that contribute to kidney disease, and the myriad altered cellular functions that occur in the setting of renal insufficiency. We are pleased that use of the Center has resulted in the publication of hundreds of articles in journals that include: American Journal of Physiology, the EMBO Journal, Journal of the American Society for Nephrology, Journal of Biological Chemistry, Molecular Biology of the Cell, and Proceedings of the National Academy of Sciences.

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Publications 2008:

Carattino MD, Hughey RP, Kleyman TR. Proteolytic processing of the epithelial sodium channel gamma subunit has a dominant role in channel activation. J Biol Chem. 283:25290-5, 2008. PMC2533072

Khandelwal P, Ruiz WG, Balestreire-Hawryluk E, Weisz OA, Goldenring JR, Apodaca G. Rab 11a dependent exocytosis of discoidal/fusiform vesicles in bladder umbrella cells. Proc Natl Acad Sci U S A. 105:15773-8, 2008. PMC257297

Passero CJ, Mueller GM, Rondon-Berrios H, Tofovic SP, Hughey RP, Kleyman TR. Plasmin activates epithelial Na+ channels by cleaving the gamma subunit. J Biol Chem. 283:36586-91, 2008. PMC2605981


Publications 2009:

Hallows KR, Wang H, Edinger RS, Butterworth MB, Oyster NM, Lil H, Buck J, Levin LR, Johnson JP, Pastor-Soler N. Novel regulation of epithelial Na+ transport by soluble adenlyl cyclase in kidney collecting duct cells. J Biol Chem. 284:5774-83, 2009. PMC2645828

Yu W, Khandelwal P, Apodaca G. Distinct apical and basolateral membrane requirements for stretch-induced membrane traffic at the apical surface of bladder umbrella cells. Mol Biol Cell. 20:282-95, 2009. PMC2613117

Maarouf AB, Sheng N, Chen J, Winarski KL, Okumura S, Carattino MD, Boyd CR, Kleyman TR, Sheng S. Novel Determinants of Epithelial Sodium Channel Gating within Extracellular Thumb Domains. J Biol Chem. 284:7756-65, 2009. PMC2658069

Hallows KR, Alzamora R, Li H, Gong F, Smolak C, Neumann D, Pastor-Soler NM. AMP-activated protein kinase inhibits alkaline pH- and PKA-induced apical vacuolar H+-ATPase accumulation in epididymal clear cells. Am J Physiol Cell Physiol. 296:C672-81, 2009. PMC2670645

Edeling MA, Sanker S, Shima T, Umasankar PK, Höning S, Kim HY, Davidson LA, Watkins SC, Tsang M, Owen DJ, Traub LM. Structural requirements for PACSIN/Syndapin operation during zebrafish embryonic notochord development. PLoS One 4:e8150, 2009. PMC2780292.

Fölsch H, Mattila PE, and Weisz OA. Taking the scenic route: Biosynthetic traffic to the plasma membrane in polarized epithelial cells. Traffic. 10:972-981, 2009. PMC2786770

Khandelwal P, Abraham SN, Apodaca G. Cell biology and physiology of the uroepithelium. Am J Physiol Renal Physiol. 297:F1477-501, 2009. PMC2801337.


Publications 2010:

Cui S, Guerriero CJ, Szalinski CM, Kinlough CL, Hughey RP, Weisz OA. OCRL1 function in renal epithelial membrane traffic. Am J Physiol Renal Physiol. 298:F335-45, 2010. PMC2822509.

Buck TM, Kolb AR, Boyd CR, Kleyman TR, and Brodsky JL. The endoplasmic reticulum-associated degradation of the epithelial sodium channel requires a unique complement of molecular chaperones. Mol Biol Cell. 21:1047-58, 2010. PMC2836957

Gong F, Alzamora R, Smolak C, Li H, Naveed S, Neumann D, Hallows KR, Pastor-Soler NM. Vacuolar H+-ATPase apical accumulation in kidney intercalated cells is regulated by PKS and AMP-activated protein kinase. Am J Physiol Renal Physiol. 298:F1162-R1169. 2010. PMC2867405

Myerburg, MM, King, JD, Oyster, NM, Fitch, AC, Magill, A, Baty, CJ, Watkins, SC, Kolls, JK, Pilewski, JM, Hallows, KR. AMPK agonists ameliorate sodium and fluid transport and inflammation in CF airway epithelial cells. Am J Respir Cell Mol Biol. 42: 676-84, 2010. PMC2891496

Khandelwal P, Ruiz WG, Apodaca G. Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway. EMBO J. 29:1961-75, 2010. PMC2892371

Li, H, Thali, RF, Smolak, C, Gong, F, Alzamora, R, Wallimann, T, Scholz, R, Pastor-Soler, NM, Neumann, D, Hallows, KR. Regulation of the creatine transporter by AMP-activated protein kinase in kidney epithelial cells. Am J Physiol Renal Physiol. 299: F167-77, 2010. PMC2904179

Hallows, KR, Bhalla, V, Oyster, NM, Wijngaarden, MO, Lee, JK, Li, H, Chandran, S, Xia, X, Huang, Z, Chalkley, RJ, Burlingame, AL, and Pearce, D. Phosphopeptide screen uncovers novel Nedd4-2 phosphorylation sites that potentiate its inhibition of the epithelial Na+ channel. J Biol Chem. 285: 21671-8, 2010. PMC2898378

Alzamora R, Thali RF, Gong F, Smolak C, Li H, Baty CJ, Bertrand CA, Auchli Y, Brunisholz RA, Neumann D, Hallows KR, Pastor-Soler NM. PKA regulates vacuolar H+-ATPase localization and activity via direct phosphorylation of the subunit in kidney cells. J Biol Chem. 285:24676-85, 2010. PMC2915704

Banerjee SK, Wang DW, Alzamora R, Huang XN, Pastor-Soler NM, Hallows KR, McGaffin KR, Ahmad F. SGLT1, a novel cardiac glucose transporter, mediates increased glucose uptake in PRKAG2 cardiomyopathy. J Mol Cell Cardiol. 49: 683-92, 2010. PMC2932762

Pedersen GA, Chakraborty S, Steinhauser AL, Traub LM, Madsen M. AMN Directs Endocytosis of the Intrinsic Factor-Vitamin B Receptor Cubam by Engaging ARH or Dab2. Traffic. 11:706-720. 2010.: PMC2964065

Mo D, Potter BA, Bertrand CA, Hildebrand JD, Bruns JR, and Weisz, OA. Nucleofection disrupts tight junction fence function to alter membrane polarity of renal epithelial cells. Am J Physiol Renal Physiol. 299: F1178-84, 2010. PMC2980404

Alzamora R, Gong F, Rondanino C, Lee JK, Smolak C, Pastor-Soler NM, Hallows KR. AMP-activated protein kinase inhibits KCNQ1 channels through regulation of the ubiquitin ligase Nedd4-2 in renal epithelial cells. Am J Physiol Renal Physiol. 299:F1308-19, 2010. PMC3006313

Mueller GM, Maarouf AB, Kinlough CL, Sheng N, Kashlan OB, Okumura S, Luthy S, Kleyman TR, Hughey RP. Cys-palmitoylation of the beta subunit modulates gating of the epithelial sodium channel. J Biol Chem. 285: 30453-30462, 2010. PMC2945538

Kashlan, OB, Boyd CR, Argyropoulos C, Okumora S, Hughey RP, Grabe M, Kleyman TR. Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains. J Biol Chem. 285: 35216-35223, 2010. PMC2966135


Publications 2011:

Kashlan OB, Adelman JL, Okumura S, Blobner BM, Zuzek Z, Hughey RP, Kleyman TR, Grabe M. Constraint based, homology model of the extracellular domain of the epithelial Na+ channel subunit reveals a mechanism of channel activation by proteases. J Biol Chem. 286: 649-60, 2011. PMC3013024

Passero CJ, Carattino MD, Kashlan OB, Myerburg MM, Hughey RP, Kleyman TR. Defining an inhibitory domain in the gamma subunit of the epithelial sodium channel. Am J Physiol Renal Physiol. 299: F854-61, 2010. PMC2957262

Yu W, Hill WG, Apodaca G, Zeidel ML. Expression and distribution of transient receptor potential (TRP) channels in bladder epithelium. Am J Physiol Renal Physiol. 300:F49-59, 2011. PMC3023226

Abi-Antoun T, Shi S, Tolino LA, Kleyman TR, Carattino MD. Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress. Am J Physiol Renal Physiol. 300: F1089-95, 2011. PMC3094055

Shi S, Ghosh DD, Okumura S, Carattino MD, Kashlan OB, Sheng S, Kleyman TR. Base of the thumb domain modulates epithelial sodium channel gating. J Biol Chem. 286: 14753-61, 2011. PMC3083234

Tolino LA, Okumura S, Kashlan OB, Carattino MD. Insights into the mechanism of pore opening of acid-sensing ion channel 1A. J Biol Chem. 286: 16297-307, 2011. PMC3091236

Winarski KL, Sheng N, Chen J, Kleyman TR, Sheng S. Extracellular allosteric regulatory subdomain within the gamma subunit of the epithelial Na+ channel. J Biol Chem 285: 26088-26096, 2010.

Rondanino C, Poland PA, Kinlough CL, Li H, Rbaibi Y, Myerburg MM, Al-Bataineh MM, Kashlan OB, Pastor-Soler NM, Hallows KR, Weisz OA, Apodaca G, Hughey RP. Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 2011, 301:F622-33. PMC3174547


Publications 2012:

Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P. Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine. Nat Commun. 2012 Mar 6;3:714. PMC3316889

Shi S, Blobner BM, Kashlan OB, Kleyman TR. Extracellular finger domain modulates the response of the epithelial sodium channel to shear stress. J Biol Chem. 287: 15439-44, 2012. PMC3346134
Kashlan OB, Kleyman TR. Epithelial Na+ channel regulation by cytoplasmic and extracellular factors. Exp Cell Res. 318: 1011-9, 2012. PubMed PMID: 22405998.

Kinlough CL, Poland PA, Gendler SJ, Mattila PE, Mo D, Weisz OA, Hughey RP. Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal. J Biol Chem. 2011, 286:39072-81. PMC3234732.

Passero CJ, Mueller GM, Myerburg MM, Carattino MD, Hughey RP, Kleyman TR. TMPRSS4-dependent activation of the epithelial sodium channel requires cleavage of the γ-subunit distal to the furin cleavage site. Am J Physiol Renal Physiol. 302: F1-8, 2012. PMC3251344

Chen J, Myerburg MM, Passero CJ, Winarski KL, Sheng S. External Cu2+ inhibits human epithelial Na+ channels by binding at a subunit interface of extracellular domains. J Biol Chem. 286: 27436-46, 2011. PMC3149337

Needham PG, Mikoluk K, Dhakarwal P, Khadem S, Snyder AC, Subramanya AR, Brodsky JL. The thiazide-sensitive NaCl cotransporter is targeted for chaperone-dependent ER-associated degradation. J Biol Chem. 286:43611-21, 2011. PMC3243568

Mattila PE, Youker RT, Mo D, Bruns JR, Cresawn KO, Hughey RP, Ihrke G, Weisz OA. Multiple biosynthetic trafficking routes for apically secreted proteins in MDCK cells. Traffic. 2012, 13:433-42. PMC3276681.

Kashlan, O.B., B. M. Blobner, Z. Zuzek, M.D. Carattino and T.R. Kleyman. Inhibitory tract traps the epithelial Na+ channel in a low activity conformation. J Biol Chem. 287: 20720-6, 2012. PMC3370254

Butterworth MB, Edinger RS, Silvis MR, Gallo LI, Liang X, Apodaca G, Frizzell RA, Johnson JP. Rab11b regulates the trafficking and recycling of the epithelial sodium channel (ENaC). Am J Physiol Renal Physiol. 302:F581-90, 2012. PMC3353647

Pastor-Soler NM, Hallows KR. AMP-activated protein kinase regulation of kidney tubular transport. Curr Opin Nephrol Hypertens. 21:523-33, 2012. PubMed PMID: 22801443

Mueller GM, Yan W, Copelovitch L, Jarman S, Wang Z, Kinlough CL, Tolino MA, Hughey RP, Kleyman TR, Rubenstein RC. Multiple residues in the distal C terminus of the a-subunit have roles in modulating human epithelial sodium channel activity. Am J Physiol Renal Physiol. 303:F220-8, 2012. PMC3404586

Prakasam HS, Herrington H, Roppolo JR, Jackson EK, Apodaca G. Modulation of Bladder Function by Luminal Adenosine Turnover and A1 Receptor Activation. Am J Physiol Renal Physiol. 303:F279-92, 2012. PMC3404591

King JD Jr, Lee J, Riemen CE, Neumann D, Xiong S, Foskett JK, Mehta A, Muimo R, Hallows KR. Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase. J Biol Chem. 287: 33389-400, 2012. PMC3460441

Mitra S, Lukianov S, Ruiz WG, Cianciolo Cosentino C, Sanker S, Traub LM, Hukriede NA, Apodaca G. Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity. PLoS One. 7(7):e41816, 2012. PMC3404999.

Edinger RS, Bertrand CA, Rondandino C, Apodaca GA, Johnson JP, Butterworth MB. The epithelial sodium channel (ENaC) establishes a trafficking vesicle pool responsible for its regulation. PLoS One. 7(9):e46593, 2012. PMC3460899

Chen J, Winarski KL, Myerburg MM, Pitt BR, Sheng S. Probing the structural basis of Zn2+ regulation of the epithelial Na+ channel. J Biol Chem. 287(42):35589-98. 2012. PMC3471688.

Shi S, Carattino MD, Kleyman TR. Role of the wrist domain in the response of the epithelial sodium channel to external stimuli. J Biol Chem. 287(53):44027-35. 2012. PMC3531719.

Rbaibi Y, Cui S, Mo D, Carattino M, Rohatgi R, Satlin LM, Szalinski CM, Swanhart LM, Fölsch H, Hukriede NA, Weisz OA. OCRL1 modulates cilia length in renal epithelial cells. Traffic. 13(9):1295-305. 2012. PMC3652247.

Mo D, Ihrke G, Costa SA, Brilli L, Labilloy A, Halfter W, Cianciolo Cosentino C, Hukriede NA, Weisz OA. Apical targeting and endocytosis of the sialomucin endolyn are essential for establishment of zebrafish pronephric kidney function. J Cell Sci. 125(Pt 22):5546-54. 2012. PMC3561862.


Publications 2013:

Abisambra J, Jinwal UK, Miyata Y, Rogers J, Blair L, Li X, Seguin SP, Wang L, Jin Y, Bacon J, Brady S, Cockman M, Guidi C, Zhang J, Koren J, Young ZT, Atkins CA, Zhang B, Lawson LY, Weeber EJ, Brodsky JL, Gestwicki JE, Dickey CA. Allosteric heat shock protein 70 inhibitors rapidly rescue synaptic plasticity deficits by reducing aberrant tau. Biol Psychiatry. 2013, 74:367-74. PMCID PMC3740016.

Alzamora R, Al-Bataineh MM, Liu W, Gong F, Li H, Thali RF, Joho-Auchli Y, Brunisholz RA, Satlin LM, Neumann D, Hallows KR, Pastor-Soler NM. AMP-activated protein kinase regulates the vacuolar H+-ATPase via direct phosphorylation of the A subunit (ATP6V1A) in the kidney. Am J Physiol Renal Physiol. 2013, 305:F943-56. PMCID: PMC3798744

Buck TM, Plavchak L, Roy A, Donnelly BF, Kashlan OB, Kleyman TR, Subramanya AR, Brodsky JL. The Lhs1/GRP170 chaperones facilitate the endoplasmic reticulum-associated degradation of the epithelial sodium channel. J Biol Chem. 2013, 288:18366-80. PMCID PMC3689978.

Carattino MD, Prakasam HS, Ruiz WG, Clayton DR, McGuire M, Gallo LI, Apodaca G. Bladder filling and voiding affect umbrella cell tight junction organization and function. Am J Physiol Renal Physiol. 2013, 305:F1158-68. PMCID PMC3798726.

Chen J, Kleyman TR, Sheng S. Gain-of-function variant of the human epithelial sodium channel. Am J. Physiol Renal Physiol. 2013, 304:F207-13. PMCID PMC3543625

Cheng D, Zhu X, Gillespie GD, Jackson EK. Role of RACK1 in the differential proliferative effects of neuropeptide Y1-36 and peptide YY1-36 in SHR versus WKY preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol. 2013, 304: F770-F780. PMCID PMC3602699

Cianciolo Cosentino C, Skrypnyk N, Brilli LB, Chiba T, Novitskaya T, Woods C, West J, Korotchenko VN, McDermott L, Day BW, Davidson AJ, Harris R, de Caestecker MP, and Hukriede NA. Histone Deacetylase inhibitor enhances recovery after AKI. J Am Soc Nephrol 2013, 266, 943-53. PMCID PMC3665399

Donnelly BF, Needham PG, Snyder AC, Roy A, Khadem S, Brodsky JL, Subramanya AR. Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter ER-associated degradation and biogenesis. J Biol Chem. 2013, 288, 13124-13135. PMCID PMC3642353.

Erickson CE, Gul R, Blessing CP, Nguyen J, Pulakat L, Bastepe M, Jackson E, Andresen BT. The β-blocker nebivolol is a GRK/β-arrestin biased agonist. PLOS ONE 2013, 8:e71980. PMCID PMC3748024

Feinstein TN, Yui N, Webber MJ, Wehbi VL, Stevenson HP, King JD Jr, Hallows KR, Brown D, Bouley R, Vilardaga JP. Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem. 2013, 288:27849-60. PMCID PMC3784700

Fraser SA, Choy SW, Pastor-Soler NM, Li H, Davies MR, Cook N, Katerelos M, Mount PF, Gleich K, McRae JL, Dwyer KM, van Denderen BJ, Hallows KR, Kemp BE, Power DA. AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1. Am J Physiol Renal Physiol. 2013, 305:F679-90. PMCID PMC3761205.

Guerriero CJ, Weiberth KF, Brodsky JL. Hsp70 targets a cytoplasmic quality control substrate to the San1p ubiquitin ligase. J Biol Chem. 2013, 288:18506-20. PMCID PMC3689992.

Hanna-Mitchell AT, Ruiz GW, Daneshgari F, Liu G, Apodaca G, Birder LA. Impact of diabetes mellitus on bladder uroepithelial cells. Am J Physiol Regul Integr Comp Physiol. 2013, 304:R84-93. PMCID PMC3543662.

Jackson EK, Cheng D, Jackson TC, Verrier JD, Gillespie, DG. Extracellular guanosine regulates extracellular adenosine levels. Am J Physiol Cell Physiol. 2013, 304: C406-C421. PMCID PMC3602643

Jackson EK, Cheng D, Mi Z, Verrier JD, Janesko-Feldman K, Kochanek PM. Role of CD73 in renal sympathetic neurotransmission in the mouse kidney. Physiol Rep. 2013, 1: e00057. PMCID PMC3779432

Jackson EK, Gillespie DG. Extracellular 2’,3’-cAMP-adenosine pathway in proximal tubular, thick ascending limb and collecting duct epithelial cells. Am J Physiol Renal Physiol. 2013, 304: F49-F55. PMCID PMC3543616

Jackson EK, Gillespie DG. Regulation of cell proliferation by the guanosine-adenosine mechanism: role of adenosine receptors. Physiol Rep. 2013, 1: e00024. PMCID PMC3743120

Jackson EK, Mi Z. In Vivo Cardiovascular Pharmacology of 2’,3’-cAMP, 2’-AMP, and 3’-AMP in the Rat. J Pharmacol Exp Ther. 2013, 346: 190-200. PMCID PMC3716313

Jackson TC, Verrier JD, Drabek T, Janesko-Feldman K, Gillespie DG, Uray T, Dezfulian C, Clark RS, Bayir H, Jackson EK, Kochanek PM. Pharmacological inhibition of pleckstrin homology domain leucine-rich repeat protein phosphatase is neuroprotective: differential effects of astrocytes. J Pharmacol Exp Ther. 2013, 347:516-528. PMCID PMC3807060

Khandelwal P, Prakasam HS, Clayton DR, Ruiz WG, Gallo LI, van Roekel D, Lukianov S, Peränen J, Goldenring JR, Apodaca G. A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells. Mol Biol Cell. 2013, 24:1007-19. PMCID PMC3608489

Krauson AJ, Rued AC, Carattino MD. Independent contribution of extracellular proton binding sites to ASIC1a activation. J Biol Chem. 2013, 288: 34375-83. PMCID PMC3843052

Miyata Y, Li X, Lee HF, Jinwal UK, Srinivasan SR, Seguin SP, Young ZT, Brodsky JL, Dickey CA, Sun D, Gestwicki JE. Synthesis and initial evaluation of YM-08, a blood-brain barrier permeable derivative of the heat shock protein 70 (Hsp70) inhibitor MKT-077, which reduces tau levels. ACS Chem Neurosci. 2013, 4:930-9. PMCID PMC3689201

Naylor RW, Przepiorski A, Ren Q, Yu J, Davidson AJ. HNF1beta is essential for nephron segmentation during nephrogenesis. J Am Soc Nephrol. 2013, 24:77-87. PMCID PMC3537220

Needham PG, Brodsky JL. How early studies on secreted and membrane protein quality control gave rise to the ER associated degradation (ERAD) pathway: the early history of ERAD. Biochim Biophys Acta. 2013, 1833:2447-57. PMCID PMC3723753.

Sanker S, Cirio MC, Vollmer LL, Goldberg ND, McDermott LA, Hukriede NA, and Vogt A. Development of high-content assay for kidney progenitor cell expansion in transgenic zebrafish. J. BioMol. Screen. 2013, 18:1193-202. PMCID PMC3830658

Saze Z, Schuler PJ, Hong CS, Cheng D, Jackson EK, Whiteside TL. Adenosine production by human B cells and B cell-mediated suppression of activated T cells. Blood 2013, 122: 9-18. PMCID PMC3701906

Schuler PJ, Macatangay BJC, Saze Z, Jackson EK, Riddler SA, Buchanan WG, Hilldorfer BB, Mellors JW, Whiteside TL, Rinaldo CR. CD4+CD73+ T cells are associated with lower T cell activation and CRP levels and are depleted in HIV-1 infection regardless of viral suppression. AIDS 2013, 27:1545-1555. PMCID PMC3939796

Shi S, Kleyman TR. Gamma subunit second transmembrane domain contributes to epithelial sodium channel gating and amiloride block. Am J Physiol Renal Physiol. 2013, 305:F1585-92. PMCID PMC3882368

Simon N, Bochman ML, Seguin S, Brodsky JL, Seibel WL, Schwacha A. Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. Biosci Rep. 2013, 33. PMCID PMC3791872

Szalinski CM, Guerriero CJ, Ruiz WG, Docter BE, Rbaibi Y, Pastor-Soler NM, Apodaca G, Puthenveedu MA, Weisz OA. PIP5KIβ selectively modulates apical endocytosis in polarized renal epithelial cells. PLoS One. 2013 8:e53790. PMCID PMC3547069.

Verrier JD, Jackson TC, Gillespie DG, Janesko-Feldman K, Bansal R, Nave AK, Kochanek PM, Jackson EK. Role of CNPase in the oligodendrocytic extracellular 2',3'-cAMP-adenosine pathway. GLIA. 2013, 61:1595-1606. PMCID PMC3998092

Wang Z, Subramanya A, Satlin LM, Pastor-Soler N, Carattino M, and TR Kleyman. Regulation of large conductance Ca2+- activated K+ channels by WNK4 kinase. Am J Physiol Cell Physiol. 2013, 305:C846-53, 2013. PMCID PMC3798677

Yoshida, O., Kimura, S., Jackson, E.K., Robson, S.C., Geller, D.A., Murase, N., and Thomson, A.W.: CD39 expression by hepatic myeloid dendritic cells attenuates inflammation in liver transplant ischemia-reperfusion injury. Hepatology 2013, 58: 2163-2175. PMCID: 3844081

Youker RT, Bruns JR, Costa SA, Rbaibi Y, Lanni F, Kashlan OB, Teng H, Weisz OA. Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization. Mol Biol Cell. 2013, 24:1996-2007. PMCID PMC3681702.


Publications 2014:

Al-Bataineh MM, Gong F, Marciszyn A, Myerburg MM, Pastor-Soler NM. Regulation of the Proximal Tubule Vacuolar H+-ATPase by PKA and AMP-Activated Protein Kinase. Am J Physiol Renal Physiol. 2014, 306:F981-95. PMCID PMC4010682

Apodaca G, Brown WJ. Membrane traffic research: challenges for the next decade. Front Cell Dev Biol. 2014 Sep 17;2:52. doi: 10.3389/fcell.2014.00052. eCollection 2014. PMCID PMC4207031.

Brodsky JL, Clark PL. Protein folding in the cell, from atom to organism. FASEB J. 2014 Dec;28(12):5034-8. PMID: 25451678 PMCID Exempt

Carattino MD, Mueller GM, Palmer LG, Frindt G, Rued AC, Hughey RP, Kleyman TR. Prostasin interacts with the epithelial Na+ channel and facilitates cleavage of the γ-subunit by a second protease. Am J Physiol Renal Physiol. 2014 Nov 1;307(9):F1080-7. PMCID PMC4216988

Carrisoza-Gaytán R, Liu Y, Flores D, Else D, Lee HG, Rhodes G, Sandoval R, Kleyman T, Lee F, Molitoris B, Satlin LM, Rohatgi R. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). Am J Physiol Renal Physiol 307:F195-204, 2014. PMCID PMC4152160

Chen J, Kleyman TR, Sheng S. Deletion of α-subunit exon 11 of the epithelial Na+ channel reveals a regulatory module. Am J Physiol Renal Physiol. 2014, 306:F561-7. PMCID PMC3949035

Cirio MC, de Groh ED, de Caestecker MP, Davidson AJ, Hukriede NA. Kidney regeneration: Common themes from the embryo to the adult. Pediatric Nephrology 2014, 29:553-64. PMCID: PMC3944192

Edinger RS, Coronnello C, Bodnar AJ, Laframboise WA, Benos PV, Ho J, Johnson JP, Butterworth MB. Aldosterone Regulates MicroRNAs in the Cortical Collecting Duct to Alter Sodium Transport. J Am Soc Nephrol. 2014, Nov;25(11):2445-57. PMCID PMC4214524.

Gallo LI, Liao Y, Ruiz WG, Clayton DR, Li M, Liu YJ, Jiang Y, Fukuda M, Apodaca G, Yin XM. TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. Mol Biol Cell. 2014 Nov 15;25(23):3779-97. PMCID PMC4230784.

Gidon A, Al-Bataineh MM, Jean-Alphonse FG, Stevenson HP, Watanabe T, Louet C, Khatri A, Calero G, Pastor-Soler NM, Gardella TJ, Vilardaga JP. Endosomal GPCR signaling turned off by negative feedback actions of PKA and v-ATPase. Nat Chem Biol. 2014 Sep;10(9):707-9. PMCID PMC4138287.

Hatfield SM, Kjaergaard J, Lukashev D, Belikoff B, Schreiber TH, Sethumadhavan S, Abbott R, Philbrook P, Thayer M, Shujia D, Rodig S, Kutok JL, Ren J, Ohta A, Podack ER, Karger B, Jackson EK, Sitkovsky M. Systemic oxygenation weakens the hypoxia and hypoxia inducible factor 1α-dependent and extracellular adenosine-mediated tumor protection. J Mol Med. 2014, 92: 1283-1292. PMCID PMC4247798

Hecht KA, O'Donnell AF, Brodsky JL. The proteolytic landscape of the yeast vacuole. Cell Logist. 2014, Jan 1;4(1):e28023. PMCID PMC4022603

Ireland AW, Gobillot TA, Gupta T, Seguin SP, Liang M, Resnick L, Goldberg MT, Manos-Turvey A, Pipas JM, Wipf P, Brodsky JL. Synthesis and structure–activity relationships of small molecule inhibitors of the simian virus 40 T antigen oncoprotein, an anti-polyomaviral target. Bioorg Med Chem. 2014, Nov 15;22:6490-502. PMCID PMC4293281

Jackson EK, Cheng D, Mi Z, Gillespie DG. Guanosine regulates adenosine levels in the kidney. Physiol Rep. 2014, 2: e12028. PMCID PMC4098751

Jackson EK, Cheng D, Verrier JD, Janesko-Feldman K, Kochanek PM. Interactive roles of CD73 and tissue non-specific alkaline phosphatase in the renal vascular metabolism of 5’-AMP. Am J Physiol Renal Physiol. 2014, 307: F680-F685. PMCID PMC4166734

Jackson EK, Gillespie DG, Mi Z, Cheng D, Bansal R, Janesko-Feldman K, Kochanek PM. Role of 2’,3’-cyclic nucleotide 3’-phosphodiesterase in the renal 2’,3’-cAMP-adenosine pathway. Am J Physiol Renal Physiol. 2014, 307: F14-F24. PMCID PMC4080157

Jackson EK, Mi Z. The guanosine-adenosine interaction exists in vivo. J Pharmacol Exp Ther. 2014, 350: 719-726. PMCID PMC4152880

Kleyman, TR and Myerburg MM. Proteases, ENaCs and cystic fibrosis. J Physiol. 2014, Dec 1;592(Pt 23):5145. PMCID PMC4262328.

Kolb AR, Needham PG, Rothenberg C, Guerriero CJ, Welling PA, Brodsky JL. ESCRT regulates surface expression of the Kir2.1 potassium channel. Mol Biol Cell. 2014, 25:276-89. PMCID PMC3890348

Koral K, Li H, Ganesh N, Birnbaum MJ, Hallows KR, Erkan E. Akt recruits Dab2 to albumin endocytosis in the proximal tubule. Am J Physiol Renal Physiol. 2014, Dec 15;307(12):F1380-9. PMCID PMC4269696

Labilloy A, Youker RT, Bruns JR, Kukic I, Kiselyov K, Halfter W, Finegold D, do Monte SJ, Weisz OA. Altered dynamics of a lipid raft associated protein in a kidney model of Fabry disease. Mol Genet Metab. 2014, 111:184-92. PMCID PMC3946758

Mattila PE, Raghavan V, Rbaibi Y, Baty CJ, and Weisz OA. Rab11a-positive compartments in proximal tubule cells sort fluid phase and membrane cargo. Am J Physiol Cell Physiol. 2014, 306:C441-449. PMCID PMC4042618

Morrell ED, Kellum JA, Hallows KR, Pastor-Soler NM. Epithelial transport during septic acute kidney injury. Nephrol Dial Transplant. 2014, Jul;29(7):1312-9. PMCID PMC4071050

Mukherjee A, Mueller GM, Kinlough CL, Sheng N, Wang Z, Mustafa SA, Kashlan OB, Kleyman TR, Hughey RP. Cys-Palmitoylation of the Gamma Subunit has a Dominant Role in Modulating Activity of the Epithelial Sodium Channel. J Biol Chem. 2014, 289: 14351-14359. PMCID PMC4022901

Novitskaya T, McDermott L, Zhang KX, Chiba T, Paueksakon P, Hukriede NA, de Caestecker MP. A PTBA class small molecule enhances recovery and reduces post injury fibrosis after aristolochic acid-induced kidney injury. Am J Physiol Renal Physiol. 2014, 306: F496-504. PMCID PMC3949031

Prakasam HS, Gallo LI, Li H, Ruiz WG, Hallows KR, Apodaca G. A1 adenosine receptor-stimulated exocytosis in bladder umbrella cells requires phosphorylation of ADAM17 Ser-811 and EGF receptor transactivation. Mol Biol Cell. 2014, Nov 15;25(23):3798-812. PMCID PMC4230785.

Raghavan V, Rbaibi R, Pastor-Soler N, Carattino MD, and Weisz OA. Shear stress dependent regulation of apical endocytosis in renal proximal tubule cells mediated by primary cilia. Proc Natl Acad Sci USA. 2014, 111:8506-8511. PMCID PMC4060694.

Schuler PJ, Saze Z, Hong CS, Muller L, Gillespie DG, Cheng D, Harasymczuk M, Mandapathil M, Lang S, Jackson EK, Whiteside TL. Human CD4+CD39+ regulatory T cells produce adenosine upon co-expression of surface CD73 or contact with CD73+ exosomes or CD73+ cells. Clin Exp Immunol. 2014, 177: 531-543. PMCID PMC4226604

Surlow BA, Cooley BM, Needham PG, Brodsky JL, Patton-Vogt J. Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation. J Biol Chem. 2014, Nov 7;289(45):31591-604. PMCID PMC4223355

Szalinski CM, Labilloy A, Bruns JR, Weisz OA. VAMP7 modulates ciliary biogenesis in kidney cells. PLoS One. 2014, 9:e86425. PMCID PMC3899255

Tomar R, Mudumana S, Pathak N, Hukriede NA, Drummond IA. osr1 is required for podocyte development downstream of wt1. J Am Soc Nephrol. 2014, Nov;25(11):2539-45. In press. PMCID PMC4214536

Warnock DG, Kusche-Vihrog K, Tarjus A, Sheng S, Oberleithner H, Kleyman TR, Jaisser F. Blood pressure and amiloride-sensitive Na+ channels in vascular and renal cells. Nat. Rev. Nephrol. 2014, Mar;10(3):146-57. PMCID PMC4137491

Wei Y, Liao Y, Zavilowitz B, Ren J, Liu W, Chan P, Rohatgi R, Estilo G, Jackson EK, Wang W, Satlin LM. Angiotensin type 2 receptor regulates ROMK-like K channel activity in renal cortical collecting duct during high dietary potassium adaptation. Am J Physiol Renal Physiol. 2014, 307:F833-43. PMCID PMC4187043

Yao M, Rogers NM, Csanyi G, Rodriguez AI, Ross MA, St. Croix C, Thomson AW, Pagano PJ, Isenberg JS. Matricellular Activation of Non-Phagocytic SIRP-α Stimulates NADPH Oxidase to Control Vascular Tone, Limit Blood Flow and Promote Ischemia Reperfusion Injury. J Am Soc Nephrol. 2014, Jun;25(6):1171-86. PMCID PMC4033366


Publications 2015:

An P, Brodsky JL, Pipas JM. The conserved core enzymatic activities and the distinct dynamics of polyomavirus large T antigens. Arch Biochem Biophys. 2015 May 1;573:23-31. PMCID PMC4865250

Brodsky JL, Frizzell RA. A combination therapy for cystic fibrosis. Cell. 2015 Sep 24;163(1):17. PubMed PMID: 26406363. PMCID PMC4865247

Buck TM, Brodsky JL. Escaping the endoplasmic reticulum: why does a molecular chaperone leave home for greener pastures? EMBO J. 2015 Jan 2;34(1):1-3. PMCID PMC4291476

Buck TM, Jordan R, Lyons-Weiler J, Adelman JL, Needham PG, Kleyman TR, Brodsky JL. Expression of three topologically distinct membrane proteins elicits unique stress response pathways in the yeast Saccharomyces cerevisiae. Physiol Genomics. 2015 Jun;47(6):198-214. PMID: 25759377; PMCID PMC4451388.

Chang A, Yeung S, Thakkar A, Kevin M, Huang KM, Liu, MM, Kanassatega RS, Parsa C, Orlando R., Jackson EK, Andresen BT, Huang Y. Prevention of skin carcinogenesis by the -blocker carvedilol. Cancer Prev Res. 2015, 8:27-36. PMCID PMC4289657

Chen J, Ray EC, Yates ME, Buck TM, Brodsky JL, Kinlough CL, Winarski KL, Hughey RP, Kleyman TR, Sheng S. Functional Roles of Clusters of Hydrophobic and Polar Residues in the Epithelial Na+ Channel Knuckle Domain. J Biol Chem. 2015, Oct 9;290(41):25140-50. PMCID PMC4599017.

Crum TS, Gleixner AM, Posimo JM, Mason DM, Broeren MT, Heinemann SD, Wipf P, Brodsky JL, Leak RK. Heat shock protein responses to aging and proteotoxicity in the olfactory bulb. J Neurochem. 2015 Jun;133(6):780-94. PMCID PMC4464935

Di Giovanni V, Walker KA, Bushnell D, Schaefer C, Sims-Lucas S, Puri P, Bates CM. Fibroblast growth factor receptor-Frs2α signaling is critical for nephron progenitors. Dev Biol. 2015, Apr 1;400(1):82-93. PMCID PMC4361332

Dubey RK, Finderle J, Gillespie DG, Mi Z, Rosselli M, Imthurn B, Jackson EK. Adenosine attenuates human coronary artery smooth muscle cell proliferation by inhibiting multiple signaling pathways that converge on cyclin D. Hypertension 2015, 66: 1207-1219. PMCID PMC4644125

Hatfield SM, Kjaergaard J., Lukashev D, Schreiber TH, Belikoff B, Abbott R, Sethumadhavan S, Philbrook P, Ko K, Cannici R, Thayer M, Rodig S, Kutok JL, Jackson EK, Karger B, Podack ER, Ohta A, Sitkovsky M. Immunological mechanisms of the antitumor effects of supplemental oxygenation. Science Translational Medicine 2015, 7: 277ra30. PMCID PMC4641038

He T, Brocca-Cofano E, Gillespie DG, Xu C, Stock JL, Ma G, Policicchio BB, Raehtz KD, Rinaldo CR, Apetrei C, Jackson EK, Macatangay BJC, Pandrea I. Critical role for the adenosine pathway in controlling SIV-related immune activation and inflammation in gut mucosal tissues. J Virol 2015, 89: 9616-9630. PMCID PMC4542384

Heidrich E, Carattino MD, Hughey RP, Pilewski JM, Kleyman TR, Myerburg MM. Intracellular Na+ regulates epithelial Na+ channel maturation. J Biol Chem. 2015, May 1;290(18):11569-77. PMCID PMC4416860

Jackson EK, Mi Z, Tofovic SP, Gillespie DG. Effect of dipeptidyl peptidase 4 inhibition on arterial blood pressure is context dependent. Hypertension 2015, 65: 238-249. PMCID PMC4268428

Jiang C, Veon W, Li H, Hallows KR, Roy P. Epithelial morphological reversion drives Profilin-1-induced elevation of p27(kip1) in mesenchymal triple-negative human breast cancer cells through AMP-activated protein kinase activation. Cell Cycle. 2015, 14(18):2914-23. PMCID PMC4825560

Kashlan OB, Blobner BM, Zuzek Z, Tolino M, Kleyman TR. Na+ inhibits the epithelial Na+ channel by binding to a site in an extracellular acidic cleft. J Biol Chem 2015, 290(1):568-76. PMCID PMC4281758

Kullmann FA, Birder LA, Andersson KE, Translational research and functional changes in voiding function in older adults. Clin Geriatr Med. 2015, Nov;31(4):535-48. PMCID PMC4865381

Li H, Satriano J, Thomas JL, Miyamoto S, Sharma K, Pastor-Soler NM, Hallows KR, Singh P. Interactions between HIF-1α and AMPK in the regulation of cellular hypoxia adaptation in chronic kidney disease. Am J Physiol Renal Physiol. 2015, Sep 1;309(5):F414-28. PMCID PMC4556888.

Marcoline FV, Bethel N, Guerriero CJ, Brodsky JL, Grabe M. Membrane Protein Properties Revealed through Data-Rich Electrostatics Calculations. Structure. 2015, Aug 4;23(8):1526-37. PMCID PMC4527966.

Miller RL, Denny GO, Knuepfer MM, Kleyman TR, Jackson EK, Salkoff LB, Loewy AD. Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema. Brain Research. 1601: 40-51, 2015. PMCID PMC4346418

Montalbetti N, Rued AC, Clayton DR, Ruiz WG, Bastacky SI, Prakasam HS, Eaton AF, Kullmann FA, Apodaca G, Carattino MD. Increased urothelial paracellular transport promotes cystitis. Am J Physiol Renal Physiol. 2015, Dec 15;309(12):F1070-81. PMCID PMC4683306.

Needham PG, Patel HJ, Chiosis G, Thibodeau PH, Brodsky JL. Mutations in the Yeast Hsp70, Ssa1, at P417 Alter ATP Cycling, Interdomain Coupling, and Specific Chaperone Functions. J Mol Biol. 2015, Sep 11;427(18):2948-65. PMCID PMC4569534

Newell EA, Exo JL, Verrier JD, Jackson TC, Gillespie DG, Janesko-Feldman K, Kochanek PM, Jackson EK. 2',3'-cAMP, 3'-AMP, 2'-AMP and adenosine inhibit TNF-α and CXCL10 production from activated primary murine microglia via A2A receptors. Brain Research 2015, 1594: 27-35. PMCID PMC4262711

Pastor-Soler NM, Sutton TA, Mang HE, Kinlough CL, Gendler SJ, Madsen CS, Bastacky SI, Ho J, Al-Bataineh MM, Hallows KR, Singh S, Monga SP, Kobayashi H, Haase VH, Hughey RP. Muc1 is Protective during Kidney Ischemia-Reperfusion Injury. Am J Physiol Renal Physiol. 2015, Jun 15;308(12):F1452-62. PMCID PMC4469889

Posimo JM, Weilnau JN, Gleixner AM, Broeren MT, Weiland NL, Brodsky JL, Wipf P, Leak RK. Heat shock protein defenses in the neocortex and allocortex of the telencephalon. Neurobiol Aging. 2015, May;36(5):1924-37. PMCID PMC4417007

Pradhan D, Roy S, Quiroga-Garza G, Cieply K, Mahaffey AL, Bastacky S, Dhir R, Parwani AV. Validation and utilization of a TFE3 break-apart FISH assay for Xp11.2 translocation renal cell carcinoma and alveolar soft part sarcoma. Diagn Pathol. 2015, Sep 29;10:179. PMCID PMC4587681.

Priedigkeit NM, Wolfe NW, Clark NL. Evolutionary signatures amongst disease genes permit novel methods for gene prioritization and construction of informative gene networks. PLOS Genetics. 2015, 11(2): e1004967. PMCID PMC4334549.

Prosser DC, Pannunzio AE, Brodsky JL, Thorner J, Wendland B, O'Donnell AF. α-Arrestins participate in cargo selection for both clathrin-independent and clathrin-mediated endocytosis. J Cell Sci. 2015, Nov 15;128(22):4220-34. PMCID PMC4712785.

Qian X, Li X, Ilori TO, Klein JD, Hughey RP, Li CJ, Alli AA, Guo Z, Yu P, Song X, Chen G. RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla. Front Physiol. 2015, Oct 1;6:274. PMCID PMC4590316

Rahaman MM, Reinders FG, Koes D, Nguyen AT, Mutchler SM, Sparacino-Watckins C, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ, Straub AC. Structure guided chemical modifications of propylthiouracil reveal novel small molecule inhibitors of cytochrome b5 reductase 3 that increase nitric oxide bioavailability. J Biol Chem. 2015, 290: 16861-16872. PMCID PMC4505432

Ray EC, Kleyman TR. Cutting it out: ENaC processing in the human nephron. J Am Soc Nephrol. 2015, 26:1-3. PMCID: PMC4279749.

Ray EC, Rondon-Berrios H, Boyd CR, Kleyman TR. Mechanisms of sodium retention and volume expansion in nephrotic syndrome: implications for hypertension. Adv. Chronic Kidney Dis. 2015, 22:179-184. PMCID PMC4409655

Reay DP, Bastacky SI, Wack KE, Stolz DB, Robbins PD, Clemens PR. D-Amino Acid Substitution of Peptide-Mediated NF-κB Suppression in mdx Mice Preserves Therapeutic Benefit in Skeletal Muscle, but Causes Kidney Toxicity. Mol Med. 2015, PMCID PMC4559531.

Rigassi L, Bozzolo FB, Lucchinetti E, Zaugg M, Fingerle F, Rosselli M, Imthurn B, Jackson EK, Dubey RK. 2-Methoxyestradiol blocks the RhoA/ROCK1 pathway in human aortic smooth muscle cells. Am J Physiol Endocrinol Metab 2015, 309: E995-E1007. PMCID PMC4816197

Roy A, Al-Bataineh MM, Pastor-Soler NM. Collecting duct intercalated cell function and regulation. Clin J Am Soc Nephrol. 2015, Feb 6;10(2):305-24. PMCID PMC4317747.

Roy A, Al-Qusairi L, Donnelly BF, Ronzaud C, Marciszyn AL, Gong F, Chang YP, Butterworth MB, Pastor-Soler NM, Hallows KR, Staub O, Subramanya AR. Alternatively spliced proline-rich cassettes link WNK1 to aldosterone action. J Clin Invest. 2015 Sep;125(9):3433-48. PMCID PMC4588284

Roy A, Goodman JH, Begum G, Donnelly BF, Pittman G, Weinman EJ, Sun D, Subramanya AR. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol. 2015, Feb 15;308(4):F366-76. PMCID PMC4329490.

Stolz DB, Sims-Lucas S. Unwrapping the origins and roles of the renal endothelium. Pediatr Nephrol. 2015, Jun;30(6):865-72. PMCID PMC4164630.

Tan RJ, Zhou D, Xiao L, Zhou L, Li Y, Bastacky SI, Oury TD, Liu Y. Extracellular Superoxide Dismutase Protects against Proteinuric Kidney Disease. J Am Soc Nephrol. 2015, Oct;26(10):2447-59. PMCID PMC4587687

Verrier JD, Kochanek PM, Jackson EK. Schwann cells metabolize extracellular 2’,3’-cAMP to 2’-AMP. J Pharmacol Exp Ther. 354: 175-183, 2015. PMCID PMC4518068

Wipf P, Eyer BR, Yamaguchi Y, Zhang F, Neal MD, Sodhi CP, Good M, Branca M, Prindle T Jr, Lu P, Brodsky JL, Hackam DJ. Synthesis of anti-inflammatory α-and β-linked acetamidopyranosides as inhibitors of toll-like receptor 4 (TLR4). Tetrahedron Lett. 2015, Jun3;56(23):3097-3100. PMCID PMC4518473.

Wolfe NW and Clark NL. ERC Analysis: web-based inference of gene function via Evolutionary Rate Covariation. Bioinformatics. 2015, 31(23):3835-7. PMCID PMC4751245.

Zhu X, Gillespie DG, Jackson EK. NPY1-36 and PYY1-36 activate cardiac fibroblasts: An effect enhanced by genetic hypertension and inhibition of dipeptidyl peptidase 4. Am J Physiol Heart Circ Physiol 2015, 309:H1528-H1542. PMCID PMC4666977


Publications 2016:

Al-Bataineh MM, Alzamora R, Ohmi K, Ho PY, Marciszyn AL, Gong F, Li H, Hallows KR, Pastor-Soler NM. Aurora Kinase A Activates the Vacuolar H+-ATPase (V-ATPase) in Kidney Carcinoma Cells. Am J Physiol Renal Physiol. 2016, Jun 1;310(11):F1216-28. PMCID PMC4935778

Al-Bataineh MM, Kinlough CL, Poland PA, Pastor-Soler NM, Sutton TA, Mang HE, Bastacky SI, Gendler SJ, Madsen CS, Singh S, Monga SP, Hughey RP. Muc1 enhances the β-catenin protective pathway during ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2016, Mar 15;310(6):F569-79. PMCID PMC4796271.

Al-Bataineh MM, Li H, Ohmi K, Gong F, Marciszyn AL, Naveed S, Zhu X, Neumann D, Wu Q, Cheng L, Fenton RA, Pastor-Soler NM, Hallows KR. Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F890-F900. PMCID PMC5533229

Al-Qusairi L, Basquin D, Roy A, Stifanelli M, Rajaram RD, Debonneville A, Nita I, Maillard MP, Loffing J, Subramanya AR, Staub O. Renal-tubular SGK1 deficiency causes impaired K+ excretion via the loss of regulation of NEDD4-2/WNK1 and ENaC. Am J Physiol Renal Physiol. 2016, Aug 1;311(2):F330-42. PMCID PMC5005279

Bebee TW, Sims-Lucas S, Park JW, Bushnell D, Cieply B, Xing Y, Bates CM, Carstens RP. Ablation of the epithelial-specific splicing factor Esrp1 results in ureteric branching defects and reduced nephron number. Dev Dyn. 2016 Oct;245(10):991-1000. PMCID PMC5096029.

Bhuiyan MI, Song S, Yuan H, Begum G, Kofler J, Kahle KT, Yang SS, Lin SH, Alper SL, Subramanya AR, Sun D. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab. 2016 Aug;37(8):2780-2794. PMCID PMC5536788

Carrisoza-Gaytan R, Carattino MD, Kleyman TR, Satlin LM. An unexpected journey: conceptual evolution of mechanoregulated potassium transport in the distal nephron Am J Physiol Cell Physiol. 2016 Feb 15;310(4):C243-59. PMCID PMC4838068

Chiba T, Skrypnyk NI, Skvarca LB, Penchev R, Zhang KX, Rochon ER, Fall JL, Paueksakon P, Yang H, Alford CE, Roman BL, Zhang MZ, Harris R, Hukriede NA, de Caestecker MP. Retinoic Acid Signaling Coordinates Macrophage-Dependent Injury and Repair after AKI. J Am Soc Nephrol. 2016, 27:495-508. PMCID: PMC4731115

Christensen M, Jensen JB, Jakobsen S, Jessen N, Frøkiær J, Kemp BE, Marciszyn AL, Li H, Pastor-Soler NM, Hallows KR, Nørregaard R. Renoprotective Effects of Metformin are Independent of Organic Cation Transporters 1 & 2 and AMP-activated Protein Kinase in the Kidney. Sci Rep. 2016, Oct 26;6:35952. PMCID PMC5080611

Chung WJ, Goeckeler-Fried JL, Havasi V, Chiang A, Rowe SM, Plyler ZE, Hong JS, Mazur M, Piazza GA, Keeton AB, White EL, Rasmussen L, Weissman AM, Denny RA, Brodsky JL, Sorscher EJ. Increasing the Endoplasmic Reticulum Pool of the F508del Allele of the Cystic Fibrosis Transmembrane Conductance Regulator Leads to Greater Folding Correction by Small Molecule Therapeutics. PLoS One. 2016, Oct 12;11(10):e0163615. PMCID PMC5061379

Figueiró F, Muller L., Funk, S, Jackson, EK, Battastini, AMO, Whiteside, T.L.: Phenotypic and functional characteristics of CD39high human regulatory B cells. OncoImmunology 5: e1082703, 2016. PMCID PMC4801473

Hao S, DelliPizzi A, Quiroz-Munoz M, Jiang H, Ferreri N. The EP3 receptor regulates water excretion in response to high salt intake. Am J Physiol Renal Physiol. 2016, Oct 1;311(4):F822-F829. PMCID PMC5142234

Jackson EK, Gillespie DG, Mi Z. 8-Aminoguanosine and 8-aminoguanine exert diuretic, natriuretic, glucosuric and antihypertensive activity. J Pharmacol Exp Ther. 2016, 359: 420-435. PMCID PMC5118650

Jackson EK, Menshikova EV, Mi Z, Verrier JD, Banal R, Janesko-Feldman K, Jackson TC, Kochanek PM. Renal 2’,3’-cyclic nucleotide 3’-phosphodiesterase is an important determinant of AKI severity after ischemia-reperfusion. J Am Soc Nephrol. 2016, Jul;27(7):2069-81. PMCID PMC4926972

Kanai AJ, Birder LA, Ikeda Y, Kullmann FA, Fry C. Implications for bidirectional signaling between afferent nerves and urothelial cells. Neurourol Urodyn. 2016, Feb;35(2):273-7. PMCID PMC4865379

Kharade SV, Flores D, Lindsley CW, Satlin LM, and JS Denton. ROMK inhibitor actions in the nephron probed with diuretics. Am J Physiol Renal Physiol. 2016, Apr 15;310(8):F732-F737. PMCID PMC4835929

Khurshid K, Yabes J, Weiss PM, Dharia S, Brown L, Unruh M, Jhamb M. Effect of antihypertensive medications on the severity of obstructive sleep apnea: A systematic review and meta-analysis. J Clin Sleep Med. 2016, Aug 15;12(8):1143-51. PMCID PMC4957192

Krauson AJ, Carattino MD. Thumb domain mediates acid-sensing ion channel desensitization. J Biol Chem. 2016, May 20;291(21):11407-19. PMCID PMC4900284

Manos-Turvey A, Al-Ashtal HA, Needham PG, Hartline CB, Prichard MN, Wipf P, Brodsky JL. Dihydropyrimidinones and -thiones with improved activity against human polyomavirus family members. Bioorg Med Chem Lett. 2016, Oct 15;26(20):5087-5091. PMCID PMC5050167

Maringer K, Sims-Lucas S. The multifaceted role of the renal microvasculature during acute kidney injury. Pediatr Nephrol. 2016, August;31(8):1231-40. PMCID PMC4841763.

McClure ML, Barnes S, Brodsky JL, Sorscher EJ. Trafficking and function of the cystic fibrosis transmembrane conductance regulator: a complex network of posttranslational modifications. Am J Physiol Lung Cell Mol Physiol. 2016, Oct 1;311(4):L719-L733. PMCID PMC5142128

Moulton DE, Sulzer V, Apodaca G, Byrne HM, Waters SL. Mathematical modelling of stretch-induced membrane traffic in bladder umbrella cells. J Theor Biol. 2016, Nov 21;409:115-132. PMCID: PMC5478889

Naylor RW, Skvarca LB, Thisse C, Thisse B, Hukriede NA, Davidson AJ. BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis. Nat Commun. 2016, Jul 13;7:12197. PMCID PMC4947171.

Nizar JM, Dong W, McClellan RB, Labarca M, Zhou Z, Wong J, Goens DG, Zhao M, Velarde N, Bernstein D, Pellizon M, Satlin LM, Bhalla V. Na+-sensitive elevation in blood pressure is ENaC independent in diet-induced obesity and insulin resistance. Am J Physiol Renal Physiol. 2016, May 1;310(9):F812-20. PMCID PMC4867314

Pereira EM, Labilloy A, Eshbach ML, Roy A, Subramanya AR, Monte S, Labilloy G, Weisz OA. Characterization and phosphoproteomic analysis of a human immortalized podocyte model of Fabry disease generated using CRISPR/Cas9 technology. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F1015-F1024. PMCID PMC5130460.

Puri P, Bushnell D, Schaefer CM, Bates CM. Six2creFrs2α knockout mice are a novel model of renal cystogenesis. Sci Rep. 2016, Nov 17;6:36736. PMCID PMC5113122.

Raghavan V, Weisz OA. Discerning the role of mechanosensors in regulating proximal tubule function. Am J Physiol Renal Physiol. 2016, Jan 1;310(1):F1-5. PMCID PMC4675802

Ramani K, Garg, AV, Jawale CV, Conti HR, Whibley N, Jackson EK, Shiva SS, Kolls JK, Gaffen SL, Biswas PS. The kallikrein-kinin system: A novel mediator of IL-17-driven anti-candida immunity in the kidney. PLOS Pathogens 2016, 22: e1005952. PMCID PMC5096720

Ray EC, Boyd-Shiwarski C, Kleyman TR. Why diuretics fail failing hearts. J Am Soc Nephrol. 2017, In Press. PMCID In process

Ray EC, Chen J, Kelly TN, He J, Hamm LL, Gu D, Shimmin LC, Hixson JE, Rao DC, Sheng S, Kleyman TR. Human epithelial Na+ channel missense variants identified in the GenSalt study alter channel activity. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F908-F914. PMCID PMC5130461

Sabnis AJ, Guerriero CJ, Olivas V, Sayana A, Shue J, Flanagan J, Asthana S, Paton AW, Paton JC, Gestwicki JE, Walter P, Weissman JS, Wipf P, Brodsky JL, Bivona TG. Combined chemical-genetic approach identifies cytosolic HSP70 dependence in rhabdomyosarcoma. Proc Natl Acad Sci USA. 2016, Aug 9;113(32):9015-20. PMCID PMC4987817

Schaufelberger SA, Rosselli M, Barchiesi F, Gillespie DG, Jackson EK, Dubey RK. 2-Methoxyestradiol, an endogenous 17-estradiol metabolite, inhibits microglial proliferation and activation via an estrogen receptor-independent mechanism. Am J Physiol Endocrinol Metab. 2016, 310:E313-322. PMCID PMC4773653

Sharma A, Yabes J, Al Mawed S, Wu C, Stilley C, Unruh M, Jhamb M. Impact of Cognitive Function Change on Mortality in Renal Transplant and End-Stage Renal Disease Patients. Am J Nephrol. 2016, 44(6):462-472. PMCID PMC5143182.

Shi S, Luke CJ,  Miedel MT, Silverman GA, Kleyman TR. Activation of the Caenorhabditis elegans degenerin channel by shear stress requires the MEC-10 subunit. J. Biol. Chem. 2016, Jul 1;291(27):14012-22. PMCID PMC4933161

Skrypnyk NI, Sanker S, Brilli-Skvarca L, Novitskaya T, Woods C, Chiba T, Patel K, Goldberg ND, McDermott L, Vinson PN, Calcutt MW, Huryn DM, Vernetti LA, Vogt A, Hukriede N, de Caestecker MP. Delayed treatment with PTBA analogs reduces post injury renal fibrosis after kidney injury. Am J Physiol Renal Physiol. 2016, Apr 15;310(8):F705-F716. PMCID PMC4835925.

Sneddon WB, Ruiz GW, Gallo LI, Xiao K, Zhang Q, Rbaibi Y, Weisz OA, Apodaca GL, Friedman PA. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport. J Biol Chem. 2016, Sep 2;291(36):18632-42. PMCID PMC5009241.

Song R, Preston G, Kidd L, Bushnell D, Sims-Lucas S, Bates CM, Yosypiv IV. Prorenin receptor is critical for nephron progenitors. Dev Biol. 2016, Jan 15;409(2):382-91. PMCID PMC4724493.

Tan RJ, Chartoumpekis DV, Rush BM, Zhou D, Fu H, Kensler TW, Liu Y. Keap1 hypomorphism protects against ischemic and obstructive kidney disease. Sci Rep. 2016, Nov 2;6:36185. PMCID PMC5090361.

Tofovic SP, Eman M, Salah EM, Smits GJ, Whalley ET, Ticho B, Deykin A, Jackson EK. Dual A1/A2B receptor blockade improves cardiac and renal outcomes in a rat model of heart failure with preserved ejection fraction. J Pharmacol Exp Ther. 2016, 356: 333-340. PMCID PMC4727158

Veit G, Avramescu RG, Chiang AN, Houck SA, Cai Z, Peters KW, Hong JS, Pollard HB, Guggino WB, Balch WE, Skach WR, Cutting GR, Frizzell RA, Sheppard DN, Cyr DM, Sorscher EJ, Brodsky JL, Lukacs GL. From CFTR biology toward combinatorial pharmacotherapy: expanded classification of cystic fibrosis mutations. Mol Biol Cell. 2016, Feb 1;27(3):424-33. PMCID PMC4751594

Wagner MC, Campos-Bilderback SB, Chowdhury M, Flores B, Lai X, Myslinski J, Pandit S, Sandoval RM, Wean SE, Satlin LM, Wiggins RC, Witzmann FA, Molitoris BA. Proximal tubules have the capacity to regulate uptake of albumin. J Am Soc Nephrol. 2016, Feb;27(2):482-94. PMCID PMC4731114

Walker KA, Sims-Lucas S, Bates CM. Fibroblast growth factor receptor signaling in kidney and lower urinary tract development. Pediatr Nephrol. 2016, Jun;31(6):885-95. PMCID PMC4761523.

Webb TN, Carrisoza-Gaytan R, Montalbetti N, Rued A, Roy A, Socovich AM, Subramanya AR, Satlin LM, Kleyman TR, Carattino MD. Cell-specific regulation of L-WNK1 by dietary K. Am J Physiol Renal Physiol. 2016, Jan 1;310(1):F15-26. PMCID PMC4675801.

Zhang Q, Dai H, Yatim KM, Abou-Daya K, Williams AL, Oberbarnscheidt MH, Camirand G, Rudd CE, Lakkis FG. CD8+ Effector T Cell Migration to Pancreatic Islet Grafts Is Dependent on Cognate Antigen Presentation by Donor Graft Cells. J Immunol. 2016 Aug 15;197(4):1471-6. PMCID PMC5023061.

Zhuang Q, Liu Q, Divito SJ, Zeng Q, Yatim KM, Hughes AD, Rojas-Canales DM, Nakao A, Shufesky WJ, Williams AL, Humar R, Hoffman RA, Shlomchik WD, Oberbarnscheidt MH, Lakkis FG, Morelli AE. Graft-infiltrating host dendritic cells play a key role in organ transplant rejection. Nat Commun. 2016, Aug 24;7:12623. PMCID PMC4999515.


Publications 2017:

Agrawal PB, Wang R, Li HL, Schmitz-Abe K, Simone-Roach C, Chen J, Shi J, Louie T, Sheng S, Towne MC, Brainson CF, Matthay MA, Kim CF, Bamshad M, Emond MJ, Gerard NP, Kleyman TR, Gerard C. Epithelial Sodium Channel ENaC is a modifier of the long term non-progressive phenotype associated with F508del CFTR mutations. Am. J. Respir. Cell Mol. Biol. 2017, In press. PMCID In process

Al-Qusairi L, Basquin D, Roy A, Rajaram RD, Maillard MP, Subramanya AR, Staub O. Renal Tubular Ubiquitin-Protein Ligase NEDD4-2 Is Required for Renal Adaptation during Long-Term Potassium Depletion. J Am Soc Nephrol. 2017, Aug;28(8):2431-2442. PMCID PMC28289184

Apodaca G. Role of Polarity Proteins in the Generation and Organization of Apical Surface Protrusions. Cold Spring Harb Perspect Biol. 2017 Mar 6. pii: a027813. In press. PMCID In process.

Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, Brodsky JL, Sobol RW. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. 2017 Feb 10;292(6):2470-2484. PMCID PMC5313114

Buck TM, Jordahl AS, Yates ME, Preston GM, Cook E, Kleyman TR, Brodsky JL. Interactions between intersubunit transmembrane domains regulate the chaperone-dependent degradation of an oligomeric membrane protein. Biochem J. 2017 Feb 1;474(3):357-376. PMCID PMC5423784

Carrisoza-Gaytán R, Wang L, Schreck C, Kleyman TR, Wang WH, Satlin LM. The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD). Am J Physiol Renal Physiol. 2017 Jan 1;312(1):F143-F156. PMCID PMC5283883.

Cerqueira DM, Bodnar AJ, Phua YL, Freer R, Hemker S, Walensky LD, Hukriede NA, Ho J. Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development. FASEBJ 2017, Aug;31(8):3540-3554. PMCID PMC5503708

Dai Y, Chen A, Liu R, Gu L, Sharma S, Cai W, Salem F, Salant DJ, Pippin J, Shankland S, Moeller M, Ghyselinck N, Ding X, Chuang PY, Lee K, He JC. Retinoic acid improves nephrotoxic serum-induced glomerulonephritis through activation of podocyte retinoic acid receptor alpha. Kidney Int. 2017, In press. PMCID In process

Dimitrion P, Zhi Y, Clayton D, Apodaca G, Wilcox, MR, Johnson JW, Nimgaonkar V, D’Aiuto, L. Low-density neuronal cultures from human induced pluripotent stem cells. Mol Neuropsychiatry. 2017, In press. PMCID In process

Emlet DR, Pastor-Soler N, Marciszyn A, Wen X, Gomez H, Humphries WH 4th, Morrisroe S, Volpe JK, Kellum JA. Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells. Am J Physiol Renal Physiol. 2017, Feb 1;312(2):F284-F296. PMCID PMC5336590

Eshbach ML, Kaur A, Rbaibi Y, Tejero J, Weisz OA. Hemoglobin inhibits albumin uptake by proximal tubule cells: implications for sickle cell disease. Am J Physiol Cell Physiol. 2017, Jun 1;312(6):C733-C740. PMCID PMC5494590

Eshbach ML, Weisz OA. Receptor-Mediated Endocytosis in the Proximal Tubule. Annu Rev Physiol. 2017, Feb 10;79:425-448. PMCID PMC5512543

Fan Y, Xiao W, Lee K, Salem F, Wen J, He L, Zhang J, Fei Y, Cheng D, Bao H, Liu Y, Lin F, Jiang G, Guo Z, Wang N, He JC. Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development. J Am Soc Nephrol. 2017, Jul;28(7):2007-2021. PMCID PMC5491287

Fu H, Tian Y, Zhou L, Zhou D, Tan RJ, Stolz DB, Liu Y. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol. 2017, Mar;28(3):785-801. PMCID PMC5328156.

Gleixner AM, Hutchison DF, Sannino S, Bhatia TN, Leak LC, Flaherty PT, Wipf P, Brodsky JL, Leak RK. N-acetyl-L-cysteine protects astrocytes against proteotoxicity without recourse to glutathione. Mol Pharmacol. 2017, In press. PMCID In process

Guerriero CJ, Reutter KR, Augustine AA, Preston GM, Weiberth KF, Mackie TD, Cleveland-Rubeor HC, Bethel NP, Callenberg KM, Nakatsukasa K, Grabe M, Brodsky JL. Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates. Mol Biol Cell. 2017, Jul 15;28(15):2076-2090. PMCID PMC5509421

Hukriede N, Vogt A, de Caestecker M. Drug Discovery to Halt the Progression of AKI to CKD: a case for phenotypic drug discovery in AKI. Nephron. 2017, In press. PMCID In process

Ikeda Y, Zabbarova I, Schaefer CM, Bushnell D, De Groat WC, Kanai A, Bates CM. Fgfr2 is integral for bladder mesenchyme patterning and function. Am J Physiol Renal Physiol. 2017, Apr 1;312(4):F607-F618. PMCID PMC5407073

Jackson EK, Kotermanski SE, Dubey RK, Jackson TC, Kochanek PM. Adenosine production by brain cells. J Neurochem. 2017, Jun;141(5):676-693. PMCID PMC5429183

Jackson EK, Zhang M, Cheng D. Alkaline phosphatase inhibitors attenuate renovascular responses to norepinephrine. Hypertension 2017, 69: 484-493. PMCID PMC5310812

Klemens CA, Edinger RS, Kightlinger L, Liu X, Butterworth MB. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017 Jan 6;292(1):375-385. PMCID PMC5217695.

Kleyman TR, Kashlan OB, Hughey RP. Epithelial Na+ channel regulation by extracellular and intracellular Factors. Annu. Rev. Physiol. 2017, In Press. PMCID In process

Kullmann FA, Chang HH, Gauthier C, McDonnell BM, Yeh JC, Clayton DR, Kanai AJ, de Groat WC, Apodaca GL, Birder LA. Acta Physiol. 2017, In press. PMCID In process

Kullmann FA, Clayton DR, Ruiz WG, Wolf-Johnson A, Gauthier C, Kanai AJ, Birder LA, Apodaca G. Urothelial proliferation and regeneration after spinal cord injury. Am J Physiol Renal Physiol. 2017, Jul 1;313(1):F85-F102. PMCID PMC5538841

Kullmann FA. A new player in interstitial cystitis/bladder pain syndrome: platelet-activating factor - PAF and its connection to smoking. Physiol Rep. 2017, Apr;5(7):e13235. PMCID PMC5392521

Liu X, Edinger RS, Klemens CA, Phua YL, Bodnar AJ, LaFramboise WA, Ho J, Butterworth MB. A MicroRNA Cluster miR-23-24-27 Is Upregulated by Aldosterone in the Distal Kidney Nephron Where it Alters Sodium Transport. J Cell Physiol. 2017, Jun;232(6):1306-1317. PMCID PMC5426511

Liu, R., Das, B., Xiao, W., Li, Z., Li, H., Lee, K., and He, J.C. A Novel Inhibitor of Homeodomain Interacting Protein Kinase 2 Mitigates Kidney Fibrosis through Inhibition of the TGF-β1/Smad3 Pathway. J Am Soc Nephrol. 2017, Jul;28(7):2133-2143. PMCID PMC5491283

Marrone AK, Stolz DB, Bastacky SI, Kostka D, Bodnar AJ, Ho J. MicroRNA-17~92 Is Required for Nephrogenesis and Renal Function. J Am Soc Nephrol. 2017, Jul;25(7):1440-52. PMCID PMC4073423

Montalbetti, N, Rued AC, Taiclet SN, Birder LA, Kullmann FA, Carattino MD. Urothelial tight junction barrier dysfunction sensitizes bladder afferents. eNeuro. 2017, May 24;4(3): ENEURO.0381-16. PMCID PMC5442440

Mukherjee A, Wang Z, Kinlough CL, Poland PA, Marciszyn AL, Montalbetti N, Carattino MD, Butterworth MB, Kleyman TR, Hughey RP. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem. 2017, Mar 10;292(10):4152-4163. PMCID PMC5354509.

Muller L, Simms P, Hong CS, Nishimura M, Jackson EK, Watkings S, and Whiteside TL. Human tumor-derived exosomes (TEX) are not internalized by T lymphocytes but signal via surface receptors to induce cellular responses. OncoImmunology 2017, In press. PMCID In process

Narla D, Slagle S, Schaefer C, Bushnell D, Bates CM. Loss of peri-Wolffian duct stromal Frs2α expression in mice leads to abnormal ureteric bud induction and vesicoureteral reflux, Pediatric Research. 2017, In press, PMCID In process.

Nickerson KM, Wang Y, Bastacky S, Shlomchik MJ. Toll-like receptor 9 suppresses lupus disease in Fas-sufficient MRL Mice. PLoS One. 2017 Mar 9;12(3):e0173471. PMCID PMC5344451.

O'Donnell BM, Mackie TD, Subramanya AR, Brodsky JL. Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome. J Biol Chem. 2017, Aug 4;292(31):12813-12827. PMCID PMC5546024

Pavlov TS, Levchenko V, Ilatovskaya DV, Li H, Palygin O, Pastor-Soler NM, Hallows KR, Staruschenko A. Lack of Effects of Metformin and AICAR Chronic Infusion on the Development of Hypertension in Dahl Salt-Sensitive Rats. Front Physiol. 2017, Apr 20;8:227. PMCID PMC53975

Preston GM, Brodsky JL. The evolving role of ubiquitin modification in endoplasmic reticulum-associated degradation. Biochem J. 2017, Feb 15;474(4):445-469. PMCID PMC5425155

Ray, E.C. and T.R. Kleyman. An increasingly complex relationship between salt and water. Am J Kidney Dis. 2017, In Press. PMCID In process

Shi S, Carattino MD. Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes. Bio Protoc. 2017, Apr 20;7(8): e2224. PMCID PMC5493207.

Shi, S., T.M. Buck, C.L. Kinlough, A.L. Marciszyn, R.P. Hughey, M. Chalfie, J.L. Brodsky and T.R. Kleyman. Regulation of the epithelial Na+ channel by paraoxonase-2. J. Biol. Chem. 2017, In press. PMCID In process

Unruh ML, Pankratz VS, Demko JE, Ray EC, Hughey RP, Kleyman TR. Trial of Amiloride in Type 2 Diabetes with Proteinuria. Kidney Int. Rep. 2017, In press. PMCID: In Process

Ye Z, Needham PG, Estabrooks SK, Whitaker SK, Garcia BL, Misra S, Brodsky JL, Camacho CJ. Symmetry breaking during homodimeric assembly activates an E3 ubiquitin ligase. Sci Rep. 2017, May 11;7(1):1789. PMCID PMC5431976

Zacchi LF, Dittmar JC, Mihalevic MJ, Shewan AM, Schulz BL, Brodsky JL, Bernstein KA. A novel high-throughput yeast genetic screen for factors modifying protein levels of the Early-Onset Torsion Dystonia-associated variant torsinAΔE. Dis Model Mech. 2017, In press. PMCID in process

Zhou D, Fu H, Zhang L, Zhang K, Min Y, Xiao L, Lin L, Bastacky SI, Liu Y. Tubule-Derived Wnts Are Required for Fibroblast Activation and Kidney Fibrosis. J Am Soc Nephrol. 2017, Aug;28(8):2322-2336. PMCID PMC5533232

Zhou D, Tian Y, Sun L, Zhou L, Xiao L, Tan RJ, Tian J, Fu H, Hou FF, Liu Y. Matrix Metalloproteinase-7 Is a Urinary Biomarker and Pathogenic Mediator of Kidney Fibrosis. J Am Soc Nephrol. 2017, Feb;28(2):598-611. PMCID PMC5280025.

Zhu X, Jackson EK. RACK1 regulates angiotensin II-induced contractions of SHR preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol 2017, Apr 1;312(4):F565-F576. PMCID PMC5407068


Center Director: Thomas Kleyman, MD

Center Associate Director: Ora Weisz, PhD

Physiology Core Directors: Marcelo Carattino, PhD and Lisa Satlin, MD

Animal and Translational Core Directors: Ed Jackson, PhD and Sean Stocker, PhD

Kidney Imaging Core Director: Gerard Apodaca, PhD

Model Systems and Therapeutics Directors: Jeff Brodsky, PhD and Neil Hukriede, PhD

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