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Pittsburgh Center for Kidney Research

A National Resource Center of the George M. O'Brien Kidney Resource Alliance (OKRA)

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Center Overview

Welcome to the Pittsburgh Center for Kidney Research, a national resource center of the George M. O’Brien Kidney Research Alliance. The Center is supported by a U54 grant (U54 DK137329) from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), by the University of Pittsburgh School of Medicine and Department of Medicine, and by the Icahn School of Medicine at Mount Sinai. Our Center is designed to facilitate basic and translational research, as well as training and information transfer related to kidney cell biology, physiology, and pathobiology. The components of our Center include the following:


Investigators - The Pittsburgh Center for Kidney Research supports over 100 investigators from across the Nation that study all aspects of kidney and lower urinary tract biology, physiology, and pathobiology. Our investigators have expertise that ranges from mathematics, to histology, to quantitative imaging, to physiology, to genetics, and to cell biology.

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Biomedical and Resource Development Cores - The Pittsburgh Center for Kidney Research supports three cores that seek to advance our understanding of kidney and lower urinary tract biology in normal and disease states: the Physiology Core, the Model Systems Core, and KIDNIT (Kidney Imaging: Developing Novel and Innovative Tools), a Resource Development Core. Each core provides cutting edge research tools and expert support.

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Education - The Center offers diverse educational opportunities including mini-sabbaticals, seminars, and research training for medical students, graduate students, and undergraduate students. Our Center hosts a lay education program that teaches patients with kidney disease and their families about the functions of the kidney, changes that occur as kidney function declines, and recent advances in kidney research.

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Publications - Use of our core facilities has resulted in hundreds of publications to date. Our manuscripts are published in journals that include the American Journal of Physiology, the Journal of Clinical Investigation, JCI Insight, the Journal of Biological Chemistry, the Journal of the American Association of Nephrology, Molecular Biology of the Cell, and Proceedings of the National Academy of Sciences.

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George M. O'Brien Kidney Resource Alliance (OKRA)

The NIH/NIDDK-funded George M. O’Brien Kidney Resource Alliance (OKRA) is a consortium of National Resource Centers (NRCs) whose primary purpose is to provide kidney researchers with specialized resources, tools, technologies, services, and expertise beyond those typically available in an individual lab or through institutional cores. The consortium includes seven NRCs, including the Pittsburgh Center for Kidney Research, and a National Coordinating Center. A list of all NRCs and other kidney disease centers can be found at the following link: National NRCs.

OKRA Opportunity Pool Program: The overarching goal of the OKRA Opportunity Pool Program (OKRA OPP) is to prioritize research support for early-stage investigators or investigators new to kidney research who are engaged in utilizing existing OKRA resources or developing novel tools or resources that will address scientific and technological gaps in kidney research and promote new collaborations. Although priority will be given to early-stage investigators and investigators new to kidney research, established kidney disease researchers are also welcome to apply. As an example of this funding opportunity can be found at this link: OKRA OPP 2024.

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Biomedical Cores and Center Administration

The Pittsburgh Center for Kidney Research at the University of Pittsburgh and Mount Sinai School of Medicine seeks to do the following: enhance the efficiency and productivity of a large number of kidney-related research projects currently in progress; make possible the acquisition of data for new projects; promote collaborations among center investigators; and provide tools to facilitate translational research. These functions depend on the Center's Biomedical Cores, which provide physiological, cell biological, genetic, analytical, molecular biological, and drug discovery tools, as well as model organisms. In addition, our Resource Development Core (KIDNIT) is developing new tools and techniques to explore kidney and urinary tract function and dysfunction using morphological tools All of our cores have unique resources for the renal research community and are designed to serve as a national and international resource. The Administrative Core oversees the Center's Cores and the Center's Educational Opportunities. Click on the icons below to learn more about each of the cores and services.

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  • Physiology Core

    Physiology Core

    The Physiology Core serves as a national resource for investigators who wish to study ion transporters and associated regulatory proteins using: (i) isolated tubules of defined identity; or (ii) primary cell isolates, established cell lines, iPS cell-derived kidney organoids and heterologous expression systems. The Physiology Core coordinates with our Model System Core to support in vitro studies using cultured epithelia, kidney organoids and isolated rodent tubules, and interfaces with our Resource Development/Imaging Core in using specific fluorescent tools to measure transporter expression and function. A major strength and focus of this Core is the incorporation of well-established and new, state-of-the-art techniques to facilitate investigations in the areas of kidney physiology and pathophysiology. One example is tubule perfusion, a once dying art, which the Physiology Core has now adapted to assess tubular function in novel rodent models and in nephron progenitor- and ureteric bud-derived organoids.

    Expression systems: These include Xenopus oocytes, yeast, and mammalian cells such as HEK-293 and Fisher Rat Thyroid (FRT) cells that permit direct measures of plasma membrane expression of channels as well as their electrophysiologic features, in parallel with biochemical and cell biological studies.

    Techniques: These include two-electrode voltage clamp to measure macroscopic currents in Xenopus oocytes expressing exogenous ion channels, standard voltage clamp for assessment of short-circuit current and transepithelial resistance in epithelial monolayers (including colon), and analyses of ion channel properties and function using whole cell, cell attached and excised patch clamp techniques. The Core provides an integrated approach to utilize microdissected tubules, colons and urinary bladders to address relevant questions. The Core supports functional (in vitro microperfusion of isolated segments, measurements of transepithelial ion/solute fluxes, fluorescence functional imaging of single tubular cells, patch clamp using split open tubules), biochemical (microassays of enzyme/transporter activity), cellular (localization) and molecular (quantitation of RNA and protein expression) methodologies applied to microdissected tubules in collaboration with our other Cores.

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    Request Core Use: Investigators interested in gaining access to any of our Core facilities or educational opportunities are asked to contact Dr. Carattino or Dr. Satlin.

    Core Director:
    Marcelo D. Carattino, PhD

    The Single Tubule and Microperfusion Subcore Director: Lisa Satlin, MD

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  • Model Systems Core

    Model Systems Core

    The Model Systems Core serves as a national resource for investigators who seek to advance their studies of kidney physiology, cell biology, and pathophysiology by using model systems (i.e., organoids, yeast, zebrafish, and rodents) in their research program. Our Core interfaces directly with our Physiology Core and with our Resource Development Core, ensuring an integrated path to phenotypic and physiological characterization of the model system at hand.

    Yeast Subcore: Our Yeast Subcore, led Dr. Jeffrey Brodsky, offers investigators the opportunity to use the power of yeast genetics to identify novel genes involved in the regulation of proteostasis, ion transport, and cellular health. There are multiple examples where knowledge gained from yeast and other model systems have successfully transitioned to studies in rodents. In turn, hypotheses that emerged from more complex systems have been rapidly tested in yeast.

    Epithelial and Organoid Subcore: The Epithelial and Organoid Subcore, led by Drs. Ora Weisz and Neil Hukriede, provides high-fidelity proximal tubule and distal nephron cell systems and kidney organoids, derived from induced pluripotent stem (iPS) cells, to address specific questions regarding physiology, cell biology, pharmacology, and pathophysiology in various nephron segments.

    Animal Subcore: The Animal Subcore, led by Drs. Arohan Subramanya and Roderick Tan, supports (i) an infrastructure for developing rodent models, and (ii) a comprehensive array of standard and Center-specific services to study kidney function in health and disease states. The Animal Subcore has expertise in a variety of acute and chronic kidney disease rodent models and is adept at assessing kidney function using tools that include metabolic cages and GFR measurements.

    Metabolomics Subcore: To further support kidney investigators, this Subcore offers state of the art measurements of purine metabolites. This is a vital offering, especially given the roles of purine metabolites in altering renal salt/water excretion and protect ing against kidney injury. Our center possesses the most comprehensive bank of purine heavy isotope internal standards in the world and represents a national asset. Finally, the center measures mitochondrial-related molecules such as cardiolipin, citrate synthase, rotenone-sensitive NADH oxidase, and Krebs cycle intermediates.

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    Request Core Use: Investigators interested in more information about our Core facilities or educational opportunities please contact Dr. Arohan Subramanya, Dr. Roderick Tan, or Dr. Jeff Brodsky.

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    Core Directors:

    Dr. Arohan Subramanya

    Dr. Roderick Tan

    Dr. Jeff Brodsky


    Yeast Subcore:

    Dr. Jeff Brodsky


    Epithelial and Organoid Subcore:

    Dr. Ora Weisz

    Dr. Neil Hukriede


    Animal and Metabolomic Subcores:

    Dr. Arohan Subramanya

    Dr. Roderick Tan

  • KIDNIT Imaging Resource Development Core

    KIDNIT - Resource Development Core

    Working in collaboration with the Center’s Cores and O’Brien Alliance investigators, our Resource Development Core, KIDNIT (Kidney Imaging: Developing Novel and Innovative Tools), serves as a national resource to develop and adapt new imaging tools and techniques to assess kidney and lower urinary tract function and dysfunction. The Core utilizes computer aided, highly automated, microscope based tools specifically developed to image these tissues, and supports our Center’s Biomedical Resource Cores that use physiological approaches to understand function and dysfunction of the kidney and lower urinary tract.

    Resource Development: Our team has the expertise to develop, tailor, and implement imaging studies in model cell, organ, and tissue systems. In addition to an expert faculty, KIDNIT exploits the facilities and imaging resources in the Renal-Electrolyte Division and in the Center for Biologic Imaging (CBI), one of the largest imaging facilities in the country. Its vast array of imaging tools can be adapted to address specific research questions. In addition to the CBI, Dr. Watkins directs a very well-outfitted machine shop that facilitates the development of custom-made imaging chambers or other devices (new optical platforms, roboticized devices, and control systems) needed to implement new and tailored imaging solutions.

    Recent examples of resource development: Recent examples of new techniques developed to address specific question include: (i) tools to image and reconstruct whole kidneys in 3D, (ii) methods to analyze collagen organization and structure in normal and fibrotic tissues using second harmonic imaging and iPOL microscopy, (iii) high throughput platforms to perform large-scale screening of cell and organoid form and function, (iv) tools to study kidney function by exploiting fluorescent life-time imaging and fluorescent resonance energy transfer, and (v) the design and manufacture of novel microfluidic chambers.

    Tools: Examples of research tools that can be adapted for kidney research use:

    Standard upright light microscopes:
    1 Olympus BX51 light microscope (Brightfield, darkfield, epifluorescence, DIC)
    3 Olympus Provis light microscopes (Brightfield, darkfield, epifluorescence, DIC)
    1 TissueGnostics automated slide scanning microscope (epifluorescence, Brightfield)
    2 Nikon 90i Upright microscope (Automated XYZ, Brightfield, darkfield, epifluorescence, DIC)

    Slide scanning microscopes:
    1 Nikon Bioslide, slide scanning microscope (150 slides, brightfield and fluorescence)
    1 Olympus VS200 slide scanning microscope (210 slides, brightfield and fluorescence)
    2 Nikon NiE Upright microscopes with slide scanners (Automated XYZ, Brightfield, darkfield, epifluorescence DIC)

    Confocal microscopes (fixed tissue and live cell):
    4 Caliber RS-G4 high-speed ribbon-scanning confocals
    6 Nikon Sweptfield confocal microscopes (TI inverted, all equipped for widefield and confocal high-throughput screening)
    1 Optiscan Confocal Endoscope/Microscope
    2 Nikon A1R confocal microscopes
    1 Olympus FV3000 confocal microscope
    2 Nikon A1RS confocal microscopes
    3 Nikon A1 confocal microscopes
    1 Nikon Ti system equipped with a Yokagawa Spinning Disc scan head and environmental chamber
    2 Olympus FV1000 Confocal Microscopes (Inverted) 1 Olympus FV1000 Confocal Microscopes (Upright)
    1 Nikon upright multimode 90i with spinning disk confocal and automated widefield imaging

    Multiphoton microscopes (all Nikon systems fully equipped for intravital imaging):
    2 Nikon A1R Multiphoton Microscopes (with 1kx1k resonant scanning)1 Olympus FluoView MPE Multiphoton Microscope
    1 Nikon AX Multiphoton microscope with 2kx2k scanner

    Widefield Live Cell microscopes (all with environmental chambers and for specialized applications including TIRF):
    2 Multimode Widefield/TIRF/ microscopes (one with microinjection capabilities)
    3 Multimode microscopes ((Automated XY, Ratioing) (two with microinjection capabilities)
    1 Nikon Ti inverted live cell system in the BSL 3 facility fully equipped for live and fixed cell imaging of pathogenic material.

    Incubator Microscopes:
    2 Incucyte S3 “incubator microscopes”
     
    Super-Resolution Microscopes:
    Leica 3X STED super-resolution/confocal system with 3 depletion lines
    Nikon CREST Deep SIM/Live cell confocal microscope
    Home built STORM microscope (Nikon Ti, Oxxius laser launch, Photometrics Kinetix camera)

    Macro systems:
    1 Olympus Multimode (3D, 2D fluorescence, high speed) dissecting microscope
    2 Olympus SXZ12 Dissecting Microscopes for MPE prep surgery
    1 Olympus SXZ-ILLD100 Dissecting Microscope

    Light Sheet microscopes:
    1 III Lattice light sheet microscope
    1 Home brew Meso-Spim microscope (generation 5)
     
    Electron Microscopes/Equipment:
    1 JEOL 1400 FLASH Transmission Electron microscope with high resolution digital camera
    1 JEOL 1400 Transmission Electron microscope with high resolution AMT digital camera
    1 JEOL 9335 Field Emission Gun SEM with backscatter detector
    1 JEOL 7800F PRIME Field Emission Gun SEM with transmitted, 2x secondary and backscatter detector
    1 JEOL JFD-V-TP freeze fracture, freeze etch, rotary shadowing, platinum replica system
    1 Leica EM PACT2 High Pressure Freezer
    1 Leica EM FSP Freeze Substitution System
    1 Cressington 108Auto Sputter coater (Gold-palladium)
    1 Cressington 108Carbon/A Carbon coater
    1 Leica EM CPD030 Critical Point Dryer

    Microtomes:
    4 Micro cryostats
    6 Reichert Ultracut (R, S, E Ultramicrotomes 1 with cryokit)

    Image analysis:
    10-gigabit networking throughout the Center.
    5.7 petabytes of high-performance parallel storage space with 60 gigabits of aggregated bandwidth.
    100 terabytes of low-latency high-performance solid-state storage with 40 gigabits of bandwidth.
    Associated, dedicated cloud storage for web-based data distribution.
    58 Pentium-based PCs all with Photoshop, Imaris, Metamorph, NIS Elements, most with deconvolution capabilities 
    1 30-device compute cluster (each with 96gb ram and 16 analysis cores)
    1 large multiprocessor workstation (32 core, 1Tb RAM, 56Tb SSD, RTX 2080 GPU).
    1 GPU computational server with (8 RTX 2080, 32 cores, 128GB RAM, 8 TB NVME SSD)
    4 dedicated workstations with TGX low-latency remote desktop solution for image visualization and processing  

    Miscellaneous:
    Multiple CCD, EM CCD, and sCMOS cameras for light microscopes. Multiple (15) live cell chambers and Syringe Pumps for media perfusion. Miscellaneous small light (inverted phase) microscopes, PCR machine, assorted protein and nucleic acid gel running apparati and power supplies. Tissue culture facilities: 2 Tissue culture hoods, 1 Hypoxic/Hyperoxic incubator, 4 x CO2 tissue culture incubators, water baths, 5 table-top microfuges, 2x tissue culture refrigerated centrifuges, Cytospin centrifuge, clinical centrifuge, pH Meter, 2 x digital balances, refrigerated high-speed (26K x g) microfuge, Beckman Airfuge and EM90 and A100 rotors. Access to departmental cold room, dark room with x-ray developer, preparative centrifuges, ultracentrifuges and rotors.



     

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    Request Core Use: Investigators interested in gaining access to KIDNIT or its educational opportunities are asked to contact Dr. Apodaca or Dr. Watkins.

  • Administrative Core

    Administrative Core

    The Administrative Core is responsible for the scientific and administrative operations of the Center. The Center Directors meet regularly with the Core/Subcore leaders to review the functioning of Center Cores as well as discuss emerging methodologies in physiology and cell biology that could be incorporated into our Cores. Our Center and Core Directors work to increase awareness of our Center among the kidney research community and greater research community through social media, our website, newsletters, and publications.

    The Administrative Core hosts a summer student enrichment program (SSEP), led by Dr. Kashlan, and a lay enrichment program, led by Dr. Jhamb. Our SSEP will support 5 undergraduate students for ten weeks of research each summer. Our Center hosts a lay education program (via Zoom) to inform patients with kidney disease and their families about the many roles of the kidney, changes that occur as kidney function declines and advances in kidney research. The Center also hosts biannual lay cooking demonstrations focusing on tasty, plant-based meals that are low in sodium, potassium and phosphate.   

    Center Director: Thomas Kleyman, MD

    Center Associate Director: Ora Weisz, PhD

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    Center Director: Thomas Kleyman, MD

    Center Associate Director: Ora Weisz, PhD

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    Physiology Core

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    Model Systems Core

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    KIDNIT Imaging Resource Development Core

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    Administrative Core

Education

The Pittsburgh Center for Kidney Research is dedicated to providing education, training, and enrichment opportunities. Mini-sabbaticals and other instructional opportunities are available to new and established investigators, post-doctoral fellows, medical students, graduate students, and undergraduate students with the goal of bringing new technologies into the laboratories of investigators to broaden and enhance their research activities. The Center also supports summer research experiences for medical students and undergraduate students.

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  • Mini Sabbaticals

    Each Core offers 1 to 2 week “mini-sabbaticals”, where attendees work in one of the Cores to learn specific techniques. For example, our Physiology Core offers three to six month sabbaticals for scientists or trainees interested in acquiring the skills needed for successful microperfusion of isolated tubules for measurement of transport or functional fluorescence imaging. Our Center will assist investigators in arranging mini-sabbaticals, including housing arrangements.

    For further information, contact Dr. Thomas Kleyman or Dr. Ora Weisz.

  • Undergraduate Student, Graduate Student, and Medical Student Research Experience and Training

    Undergraduate Students: The Administrative Core hosts a Summer Student Enrichment Program (SSEP), led by Dr. Kashlan that will support 5 undergraduate students for ten weeks of research each summer.

    Graduate Students: Faculty members associated with the Center have a long track-record of training graduate students in biomedical research. Prospective students interested in Ph.D. training are encouraged to apply to the following Ph.D. training programs: 

    The Interdisciplinary Biomedical Graduate Program (www.gradbiomed.pitt.edu), with an emphasis in Cell Biology and Molecular Physiology (www.cbmp.pitt.edu). 

    Integrative Systems Biology (www.isb.pitt.edu).

    Medical Students: Over eighty University of Pittsburgh medical students perform research during the summer between their first and second year. Dr. Kleyman is the PI of a T35 training grant (T35 DK065521) that provides support for medical students to perform research over the summer under the auspices of a faculty member. This support mechanism encourages students to perform research under the direction of one of our Center investigators. Students working in a kidney research laboratory participate in our renal division educational activities, which includes a series of lectures for our renal fellows that focuses on kidney physiology, pathophysiology, and disease management. They also attend a faculty-facilitated discussion on authorship issues, a practical talk about poster and platform presentations, writing abstracts, and ethical issues in research. The relevance of biomedical research is discussed, using a number of examples of molecular biologic discoveries and gene transfer in inherited immunodeficiency disorders, diabetes, and cystic fibrosis, to illustrate the importance of translating discoveries at the bench to the beside in order to improve clinical care and advance medicine.

  • Meetings, Retreats, and Symposia

    Annual Acute Kidney Injury Retreat: The Center co-hosts an annual, day-long retreat with the Department of Critical Care Medicine to foster a cross-campus dialogue focused on AKI with a goal of identifying common areas of interest and developing collaborations. University of Pittsburgh faculty, senior fellows, and trainees present ongoing research and receive feedback from attendees. Experts external to our program are invited to participate in our retreat and present their own research. Past keynote speakers have included Ray Harris, Pierre Dagher, Volker Hasse, Claudio Ronco, Benjamin Humphreys, Anupam Agarwal, Kathleen Liu, and Andrew Rule.  

    The Local Traffic Symposium: This one-day meeting, sixteen years in the running, explores the cell biology of membrane biogenesis and traffic. Participants include regional investigators, students, and postdocs from the University of Pittsburgh and Carnegie Mellon University as well as researchers and guest speakers from other universities in Pennsylvania, Ohio, and the NIH. The meeting includes student presentations, faculty talks, and a keynote address that provides a forum for students, faculty, and research support staff with an interest in membrane traffic to interact and share their recent findings on protein folding, ER degradation, secretory and endocytic traffic. Recent keynote speakers include James Rothman, Scott Emr, and Lois Weisman. Support for the meeting is provided by the Dean of the Medical School, the Department of Cell Biology, the Renal-Electrolyte Division, and the O’Brien Kidney Center. Attendance at this meeting typically exceeds 120 and is free for attendees.

    Ubiquitin-Proteosome Meeting: An annual day-long Pittsburgh Ubiquitin-Proteasome Meeting is organized by investigators from the Department of Biological Sciences (Brodsky), the School of Medicine/Hillman Cancer Center (Wan), and the Department of Pharmaceutical Sciences (Kwan) at the University of Pittsburgh. The meeting brings together the PIs and personnel from the ~10 groups in the Pittsburgh area, as well as researchers from other areas (e.g., Washington DC and West Virginia) whose work relates to various aspects of cellular protein quality control and the ubiquitin-proteasome pathway. The invited guest lecture for the 2012 meeting was Ron Kopito from Stanford University. Support is provided by the University of Pittsburgh School of Medicine.

    Annual Summer Workshop in Teluride: Dr. Kleyman has been co-organizing an annual week-long summer workshop on Epithelial Physiology and Cell Biology held in Telluride, Colorado, under the auspices of the Telluride Science Research Center for the past 10 years. The goal of this workshop is to bring together experts who present recent findings and generate discussions in the following areas: (i) structure and regulation of epithelial ion channels; (ii) structure and regulation of ion cotransporters, exchangers and pumps; and (iii) protein trafficking in epithelia. We encourage Core Directors/co-Directors, scientists supported by Pilot andFeasibility funding, as well as scientists utilizing our Core facilities to participate in this meeting. 

  • Community Education: Kidney Lay Education Program
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    The objective of our Kidney Lay Education Program is to educate patients with kidney disease and their families about the roles of the kidney, the changes that occur as kidney function declines, and new advances in kidney research that may help their conditions. Organized by Dr. Manisha Jamb, our one-hour lay sessions are held on a bi-annual basis and presented via Zoom. In the past, they have coincided with our Kidney Injury Symposium or as part of the National Kidney Foundation educational seminars for patients with kidney disease and their care partners.
     

    We also offer hands-on cooking classes for patients with kidney disease. Patients with kidney disease have complex dietary needs due to co-existing comorbidities. The goal of the cooking classes are to teach cooking skills, with a focus on plant-based meals that are low in salt, potassium and phosphate, and are affordable, delicious and easy to prepare. The classes are led by a professional chef (Barbara Kleyman) and a renal dietitian, and offered every 4-6 months for patients with kidney disease and their families. We are working with our local NKF and our Health System to increase awareness of our cooking classes among our lay community.



    For further information, contact Dr. Thomas Kleyman or Dr. Ora Weisz.

Publications

A major goal of our Center is to advance our knowledge of normal kidney function, cellular mechanisms that contribute to kidney disease, and the myriad altered cellular functions that occur in the setting of renal insufficiency. We are pleased that use of the Center has resulted in the publication of hundreds of articles in journals that include: American Journal of Physiology, the EMBO Journal, Journal of the American Society for Nephrology, Journal of Biological Chemistry, Molecular Biology of the Cell, and Proceedings of the National Academy of Sciences.

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2008 

Publications 2008:

Carattino MD, Hughey RP, Kleyman TR. Proteolytic processing of the epithelial sodium channel gamma subunit has a dominant role in channel activation. J Biol Chem. 283:25290-5, 2008. PMC2533072

Khandelwal P, Ruiz WG, Balestreire-Hawryluk E, Weisz OA, Goldenring JR, Apodaca G. Rab 11a dependent exocytosis of discoidal/fusiform vesicles in bladder umbrella cells. Proc Natl Acad Sci U S A. 105:15773-8, 2008. PMC257297

Passero CJ, Mueller GM, Rondon-Berrios H, Tofovic SP, Hughey RP, Kleyman TR. Plasmin activates epithelial Na+ channels by cleaving the gamma subunit. J Biol Chem. 283:36586-91, 2008. PMC2605981

2009 

Publications 2009:

Hallows KR, Wang H, Edinger RS, Butterworth MB, Oyster NM, Lil H, Buck J, Levin LR, Johnson JP, Pastor-Soler N. Novel regulation of epithelial Na+ transport by soluble adenlyl cyclase in kidney collecting duct cells. J Biol Chem. 284:5774-83, 2009. PMC2645828

Yu W, Khandelwal P, Apodaca G. Distinct apical and basolateral membrane requirements for stretch-induced membrane traffic at the apical surface of bladder umbrella cells. Mol Biol Cell. 20:282-95, 2009. PMC2613117

Maarouf AB, Sheng N, Chen J, Winarski KL, Okumura S, Carattino MD, Boyd CR, Kleyman TR, Sheng S. Novel Determinants of Epithelial Sodium Channel Gating within Extracellular Thumb Domains. J Biol Chem. 284:7756-65, 2009. PMC2658069

Hallows KR, Alzamora R, Li H, Gong F, Smolak C, Neumann D, Pastor-Soler NM. AMP-activated protein kinase inhibits alkaline pH- and PKA-induced apical vacuolar H+-ATPase accumulation in epididymal clear cells. Am J Physiol Cell Physiol. 296:C672-81, 2009. PMC2670645

Edeling MA, Sanker S, Shima T, Umasankar PK, Höning S, Kim HY, Davidson LA, Watkins SC, Tsang M, Owen DJ, Traub LM. Structural requirements for PACSIN/Syndapin operation during zebrafish embryonic notochord development. PLoS One 4:e8150, 2009. PMC2780292.

Fölsch H, Mattila PE, and Weisz OA. Taking the scenic route: Biosynthetic traffic to the plasma membrane in polarized epithelial cells. Traffic. 10:972-981, 2009. PMC2786770

Khandelwal P, Abraham SN, Apodaca G. Cell biology and physiology of the uroepithelium. Am J Physiol Renal Physiol. 297:F1477-501, 2009. PMC2801337.

2010 

Publications 2010:

Cui S, Guerriero CJ, Szalinski CM, Kinlough CL, Hughey RP, Weisz OA. OCRL1 function in renal epithelial membrane traffic. Am J Physiol Renal Physiol. 298:F335-45, 2010. PMC2822509.

Buck TM, Kolb AR, Boyd CR, Kleyman TR, and Brodsky JL. The endoplasmic reticulum-associated degradation of the epithelial sodium channel requires a unique complement of molecular chaperones. Mol Biol Cell. 21:1047-58, 2010. PMC2836957

Gong F, Alzamora R, Smolak C, Li H, Naveed S, Neumann D, Hallows KR, Pastor-Soler NM. Vacuolar H+-ATPase apical accumulation in kidney intercalated cells is regulated by PKS and AMP-activated protein kinase. Am J Physiol Renal Physiol. 298:F1162-R1169. 2010. PMC2867405

Myerburg, MM, King, JD, Oyster, NM, Fitch, AC, Magill, A, Baty, CJ, Watkins, SC, Kolls, JK, Pilewski, JM, Hallows, KR. AMPK agonists ameliorate sodium and fluid transport and inflammation in CF airway epithelial cells. Am J Respir Cell Mol Biol. 42: 676-84, 2010. PMC2891496

Khandelwal P, Ruiz WG, Apodaca G. Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway. EMBO J. 29:1961-75, 2010. PMC2892371

Li, H, Thali, RF, Smolak, C, Gong, F, Alzamora, R, Wallimann, T, Scholz, R, Pastor-Soler, NM, Neumann, D, Hallows, KR. Regulation of the creatine transporter by AMP-activated protein kinase in kidney epithelial cells. Am J Physiol Renal Physiol. 299: F167-77, 2010. PMC2904179

Hallows, KR, Bhalla, V, Oyster, NM, Wijngaarden, MO, Lee, JK, Li, H, Chandran, S, Xia, X, Huang, Z, Chalkley, RJ, Burlingame, AL, and Pearce, D. Phosphopeptide screen uncovers novel Nedd4-2 phosphorylation sites that potentiate its inhibition of the epithelial Na+ channel. J Biol Chem. 285: 21671-8, 2010. PMC2898378

Alzamora R, Thali RF, Gong F, Smolak C, Li H, Baty CJ, Bertrand CA, Auchli Y, Brunisholz RA, Neumann D, Hallows KR, Pastor-Soler NM. PKA regulates vacuolar H+-ATPase localization and activity via direct phosphorylation of the subunit in kidney cells. J Biol Chem. 285:24676-85, 2010. PMC2915704

Banerjee SK, Wang DW, Alzamora R, Huang XN, Pastor-Soler NM, Hallows KR, McGaffin KR, Ahmad F. SGLT1, a novel cardiac glucose transporter, mediates increased glucose uptake in PRKAG2 cardiomyopathy. J Mol Cell Cardiol. 49: 683-92, 2010. PMC2932762

Pedersen GA, Chakraborty S, Steinhauser AL, Traub LM, Madsen M. AMN Directs Endocytosis of the Intrinsic Factor-Vitamin B Receptor Cubam by Engaging ARH or Dab2. Traffic. 11:706-720. 2010.: PMC2964065

Mo D, Potter BA, Bertrand CA, Hildebrand JD, Bruns JR, and Weisz, OA. Nucleofection disrupts tight junction fence function to alter membrane polarity of renal epithelial cells. Am J Physiol Renal Physiol. 299: F1178-84, 2010. PMC2980404

Alzamora R, Gong F, Rondanino C, Lee JK, Smolak C, Pastor-Soler NM, Hallows KR. AMP-activated protein kinase inhibits KCNQ1 channels through regulation of the ubiquitin ligase Nedd4-2 in renal epithelial cells. Am J Physiol Renal Physiol. 299:F1308-19, 2010. PMC3006313

Mueller GM, Maarouf AB, Kinlough CL, Sheng N, Kashlan OB, Okumura S, Luthy S, Kleyman TR, Hughey RP. Cys-palmitoylation of the beta subunit modulates gating of the epithelial sodium channel. J Biol Chem. 285: 30453-30462, 2010. PMC2945538

Kashlan, OB, Boyd CR, Argyropoulos C, Okumora S, Hughey RP, Grabe M, Kleyman TR. Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains. J Biol Chem. 285: 35216-35223, 2010. PMC2966135

2011 

Publications 2011:

Kashlan OB, Adelman JL, Okumura S, Blobner BM, Zuzek Z, Hughey RP, Kleyman TR, Grabe M. Constraint based, homology model of the extracellular domain of the epithelial Na+ channel subunit reveals a mechanism of channel activation by proteases. J Biol Chem. 286: 649-60, 2011. PMC3013024

Passero CJ, Carattino MD, Kashlan OB, Myerburg MM, Hughey RP, Kleyman TR. Defining an inhibitory domain in the gamma subunit of the epithelial sodium channel. Am J Physiol Renal Physiol. 299: F854-61, 2010. PMC2957262

Yu W, Hill WG, Apodaca G, Zeidel ML. Expression and distribution of transient receptor potential (TRP) channels in bladder epithelium. Am J Physiol Renal Physiol. 300:F49-59, 2011. PMC3023226

Abi-Antoun T, Shi S, Tolino LA, Kleyman TR, Carattino MD. Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress. Am J Physiol Renal Physiol. 300: F1089-95, 2011. PMC3094055

Shi S, Ghosh DD, Okumura S, Carattino MD, Kashlan OB, Sheng S, Kleyman TR. Base of the thumb domain modulates epithelial sodium channel gating. J Biol Chem. 286: 14753-61, 2011. PMC3083234

Tolino LA, Okumura S, Kashlan OB, Carattino MD. Insights into the mechanism of pore opening of acid-sensing ion channel 1A. J Biol Chem. 286: 16297-307, 2011. PMC3091236

Winarski KL, Sheng N, Chen J, Kleyman TR, Sheng S. Extracellular allosteric regulatory subdomain within the gamma subunit of the epithelial Na+ channel. J Biol Chem 285: 26088-26096, 2010.
PMC2924010

Rondanino C, Poland PA, Kinlough CL, Li H, Rbaibi Y, Myerburg MM, Al-Bataineh MM, Kashlan OB, Pastor-Soler NM, Hallows KR, Weisz OA, Apodaca G, Hughey RP. Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 2011, 301:F622-33. PMC3174547

2012 

Publications 2012:

Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P. Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine. Nat Commun. 2012 Mar 6;3:714. PMC3316889

Shi S, Blobner BM, Kashlan OB, Kleyman TR. Extracellular finger domain modulates the response of the epithelial sodium channel to shear stress. J Biol Chem. 287: 15439-44, 2012. PMC3346134
Kashlan OB, Kleyman TR. Epithelial Na+ channel regulation by cytoplasmic and extracellular factors. Exp Cell Res. 318: 1011-9, 2012. PubMed PMID: 22405998.

Kinlough CL, Poland PA, Gendler SJ, Mattila PE, Mo D, Weisz OA, Hughey RP. Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal. J Biol Chem. 2011, 286:39072-81. PMC3234732.

Passero CJ, Mueller GM, Myerburg MM, Carattino MD, Hughey RP, Kleyman TR. TMPRSS4-dependent activation of the epithelial sodium channel requires cleavage of the γ-subunit distal to the furin cleavage site. Am J Physiol Renal Physiol. 302: F1-8, 2012. PMC3251344

Chen J, Myerburg MM, Passero CJ, Winarski KL, Sheng S. External Cu2+ inhibits human epithelial Na+ channels by binding at a subunit interface of extracellular domains. J Biol Chem. 286: 27436-46, 2011. PMC3149337

Needham PG, Mikoluk K, Dhakarwal P, Khadem S, Snyder AC, Subramanya AR, Brodsky JL. The thiazide-sensitive NaCl cotransporter is targeted for chaperone-dependent ER-associated degradation. J Biol Chem. 286:43611-21, 2011. PMC3243568

Mattila PE, Youker RT, Mo D, Bruns JR, Cresawn KO, Hughey RP, Ihrke G, Weisz OA. Multiple biosynthetic trafficking routes for apically secreted proteins in MDCK cells. Traffic. 2012, 13:433-42. PMC3276681.

Kashlan, O.B., B. M. Blobner, Z. Zuzek, M.D. Carattino and T.R. Kleyman. Inhibitory tract traps the epithelial Na+ channel in a low activity conformation. J Biol Chem. 287: 20720-6, 2012. PMC3370254

Butterworth MB, Edinger RS, Silvis MR, Gallo LI, Liang X, Apodaca G, Frizzell RA, Johnson JP. Rab11b regulates the trafficking and recycling of the epithelial sodium channel (ENaC). Am J Physiol Renal Physiol. 302:F581-90, 2012. PMC3353647

Pastor-Soler NM, Hallows KR. AMP-activated protein kinase regulation of kidney tubular transport. Curr Opin Nephrol Hypertens. 21:523-33, 2012. PubMed PMID: 22801443

Mueller GM, Yan W, Copelovitch L, Jarman S, Wang Z, Kinlough CL, Tolino MA, Hughey RP, Kleyman TR, Rubenstein RC. Multiple residues in the distal C terminus of the a-subunit have roles in modulating human epithelial sodium channel activity. Am J Physiol Renal Physiol. 303:F220-8, 2012. PMC3404586

Prakasam HS, Herrington H, Roppolo JR, Jackson EK, Apodaca G. Modulation of Bladder Function by Luminal Adenosine Turnover and A1 Receptor Activation. Am J Physiol Renal Physiol. 303:F279-92, 2012. PMC3404591

King JD Jr, Lee J, Riemen CE, Neumann D, Xiong S, Foskett JK, Mehta A, Muimo R, Hallows KR. Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase. J Biol Chem. 287: 33389-400, 2012. PMC3460441

Mitra S, Lukianov S, Ruiz WG, Cianciolo Cosentino C, Sanker S, Traub LM, Hukriede NA, Apodaca G. Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity. PLoS One. 7(7):e41816, 2012. PMC3404999.

Edinger RS, Bertrand CA, Rondandino C, Apodaca GA, Johnson JP, Butterworth MB. The epithelial sodium channel (ENaC) establishes a trafficking vesicle pool responsible for its regulation. PLoS One. 7(9):e46593, 2012. PMC3460899

Chen J, Winarski KL, Myerburg MM, Pitt BR, Sheng S. Probing the structural basis of Zn2+ regulation of the epithelial Na+ channel. J Biol Chem. 287(42):35589-98. 2012. PMC3471688.

Shi S, Carattino MD, Kleyman TR. Role of the wrist domain in the response of the epithelial sodium channel to external stimuli. J Biol Chem. 287(53):44027-35. 2012. PMC3531719.

Rbaibi Y, Cui S, Mo D, Carattino M, Rohatgi R, Satlin LM, Szalinski CM, Swanhart LM, Fölsch H, Hukriede NA, Weisz OA. OCRL1 modulates cilia length in renal epithelial cells. Traffic. 13(9):1295-305. 2012. PMC3652247.

Mo D, Ihrke G, Costa SA, Brilli L, Labilloy A, Halfter W, Cianciolo Cosentino C, Hukriede NA, Weisz OA. Apical targeting and endocytosis of the sialomucin endolyn are essential for establishment of zebrafish pronephric kidney function. J Cell Sci. 125(Pt 22):5546-54. 2012. PMC3561862.


2013 

Publications 2013:

Abisambra J, Jinwal UK, Miyata Y, Rogers J, Blair L, Li X, Seguin SP, Wang L, Jin Y, Bacon J, Brady S, Cockman M, Guidi C, Zhang J, Koren J, Young ZT, Atkins CA, Zhang B, Lawson LY, Weeber EJ, Brodsky JL, Gestwicki JE, Dickey CA. Allosteric heat shock protein 70 inhibitors rapidly rescue synaptic plasticity deficits by reducing aberrant tau. Biol Psychiatry. 2013, 74:367-74. PMCID PMC3740016.

Alzamora R, Al-Bataineh MM, Liu W, Gong F, Li H, Thali RF, Joho-Auchli Y, Brunisholz RA, Satlin LM, Neumann D, Hallows KR, Pastor-Soler NM. AMP-activated protein kinase regulates the vacuolar H+-ATPase via direct phosphorylation of the A subunit (ATP6V1A) in the kidney. Am J Physiol Renal Physiol. 2013, 305:F943-56. PMCID: PMC3798744

Buck TM, Plavchak L, Roy A, Donnelly BF, Kashlan OB, Kleyman TR, Subramanya AR, Brodsky JL. The Lhs1/GRP170 chaperones facilitate the endoplasmic reticulum-associated degradation of the epithelial sodium channel. J Biol Chem. 2013, 288:18366-80. PMCID PMC3689978.

Carattino MD, Prakasam HS, Ruiz WG, Clayton DR, McGuire M, Gallo LI, Apodaca G. Bladder filling and voiding affect umbrella cell tight junction organization and function. Am J Physiol Renal Physiol. 2013, 305:F1158-68. PMCID PMC3798726.

Chen J, Kleyman TR, Sheng S. Gain-of-function variant of the human epithelial sodium channel. Am J. Physiol Renal Physiol. 2013, 304:F207-13. PMCID PMC3543625

Cheng D, Zhu X, Gillespie GD, Jackson EK. Role of RACK1 in the differential proliferative effects of neuropeptide Y1-36 and peptide YY1-36 in SHR versus WKY preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol. 2013, 304: F770-F780. PMCID PMC3602699

Cianciolo Cosentino C, Skrypnyk N, Brilli LB, Chiba T, Novitskaya T, Woods C, West J, Korotchenko VN, McDermott L, Day BW, Davidson AJ, Harris R, de Caestecker MP, and Hukriede NA. Histone Deacetylase inhibitor enhances recovery after AKI. J Am Soc Nephrol 2013, 266, 943-53. PMCID PMC3665399

Donnelly BF, Needham PG, Snyder AC, Roy A, Khadem S, Brodsky JL, Subramanya AR. Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter ER-associated degradation and biogenesis. J Biol Chem. 2013, 288, 13124-13135. PMCID PMC3642353.

Erickson CE, Gul R, Blessing CP, Nguyen J, Pulakat L, Bastepe M, Jackson E, Andresen BT. The β-blocker nebivolol is a GRK/β-arrestin biased agonist. PLOS ONE 2013, 8:e71980. PMCID PMC3748024

Feinstein TN, Yui N, Webber MJ, Wehbi VL, Stevenson HP, King JD Jr, Hallows KR, Brown D, Bouley R, Vilardaga JP. Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem. 2013, 288:27849-60. PMCID PMC3784700

Fraser SA, Choy SW, Pastor-Soler NM, Li H, Davies MR, Cook N, Katerelos M, Mount PF, Gleich K, McRae JL, Dwyer KM, van Denderen BJ, Hallows KR, Kemp BE, Power DA. AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1. Am J Physiol Renal Physiol. 2013, 305:F679-90. PMCID PMC3761205.

Guerriero CJ, Weiberth KF, Brodsky JL. Hsp70 targets a cytoplasmic quality control substrate to the San1p ubiquitin ligase. J Biol Chem. 2013, 288:18506-20. PMCID PMC3689992.

Hanna-Mitchell AT, Ruiz GW, Daneshgari F, Liu G, Apodaca G, Birder LA. Impact of diabetes mellitus on bladder uroepithelial cells. Am J Physiol Regul Integr Comp Physiol. 2013, 304:R84-93. PMCID PMC3543662.

Jackson EK, Cheng D, Jackson TC, Verrier JD, Gillespie, DG. Extracellular guanosine regulates extracellular adenosine levels. Am J Physiol Cell Physiol. 2013, 304: C406-C421. PMCID PMC3602643

Jackson EK, Cheng D, Mi Z, Verrier JD, Janesko-Feldman K, Kochanek PM. Role of CD73 in renal sympathetic neurotransmission in the mouse kidney. Physiol Rep. 2013, 1: e00057. PMCID PMC3779432

Jackson EK, Gillespie DG. Extracellular 2’,3’-cAMP-adenosine pathway in proximal tubular, thick ascending limb and collecting duct epithelial cells. Am J Physiol Renal Physiol. 2013, 304: F49-F55. PMCID PMC3543616

Jackson EK, Gillespie DG. Regulation of cell proliferation by the guanosine-adenosine mechanism: role of adenosine receptors. Physiol Rep. 2013, 1: e00024. PMCID PMC3743120

Jackson EK, Mi Z. In Vivo Cardiovascular Pharmacology of 2’,3’-cAMP, 2’-AMP, and 3’-AMP in the Rat. J Pharmacol Exp Ther. 2013, 346: 190-200. PMCID PMC3716313

Jackson TC, Verrier JD, Drabek T, Janesko-Feldman K, Gillespie DG, Uray T, Dezfulian C, Clark RS, Bayir H, Jackson EK, Kochanek PM. Pharmacological inhibition of pleckstrin homology domain leucine-rich repeat protein phosphatase is neuroprotective: differential effects of astrocytes. J Pharmacol Exp Ther. 2013, 347:516-528. PMCID PMC3807060

Khandelwal P, Prakasam HS, Clayton DR, Ruiz WG, Gallo LI, van Roekel D, Lukianov S, Peränen J, Goldenring JR, Apodaca G. A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells. Mol Biol Cell. 2013, 24:1007-19. PMCID PMC3608489

Krauson AJ, Rued AC, Carattino MD. Independent contribution of extracellular proton binding sites to ASIC1a activation. J Biol Chem. 2013, 288: 34375-83. PMCID PMC3843052

Miyata Y, Li X, Lee HF, Jinwal UK, Srinivasan SR, Seguin SP, Young ZT, Brodsky JL, Dickey CA, Sun D, Gestwicki JE. Synthesis and initial evaluation of YM-08, a blood-brain barrier permeable derivative of the heat shock protein 70 (Hsp70) inhibitor MKT-077, which reduces tau levels. ACS Chem Neurosci. 2013, 4:930-9. PMCID PMC3689201

Naylor RW, Przepiorski A, Ren Q, Yu J, Davidson AJ. HNF1beta is essential for nephron segmentation during nephrogenesis. J Am Soc Nephrol. 2013, 24:77-87. PMCID PMC3537220

Needham PG, Brodsky JL. How early studies on secreted and membrane protein quality control gave rise to the ER associated degradation (ERAD) pathway: the early history of ERAD. Biochim Biophys Acta. 2013, 1833:2447-57. PMCID PMC3723753.

Sanker S, Cirio MC, Vollmer LL, Goldberg ND, McDermott LA, Hukriede NA, and Vogt A. Development of high-content assay for kidney progenitor cell expansion in transgenic zebrafish. J. BioMol. Screen. 2013, 18:1193-202. PMCID PMC3830658

Saze Z, Schuler PJ, Hong CS, Cheng D, Jackson EK, Whiteside TL. Adenosine production by human B cells and B cell-mediated suppression of activated T cells. Blood 2013, 122: 9-18. PMCID PMC3701906

Schuler PJ, Macatangay BJC, Saze Z, Jackson EK, Riddler SA, Buchanan WG, Hilldorfer BB, Mellors JW, Whiteside TL, Rinaldo CR. CD4+CD73+ T cells are associated with lower T cell activation and CRP levels and are depleted in HIV-1 infection regardless of viral suppression. AIDS 2013, 27:1545-1555. PMCID PMC3939796

Shi S, Kleyman TR. Gamma subunit second transmembrane domain contributes to epithelial sodium channel gating and amiloride block. Am J Physiol Renal Physiol. 2013, 305:F1585-92. PMCID PMC3882368

Simon N, Bochman ML, Seguin S, Brodsky JL, Seibel WL, Schwacha A. Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. Biosci Rep. 2013, 33. PMCID PMC3791872

Szalinski CM, Guerriero CJ, Ruiz WG, Docter BE, Rbaibi Y, Pastor-Soler NM, Apodaca G, Puthenveedu MA, Weisz OA. PIP5KIβ selectively modulates apical endocytosis in polarized renal epithelial cells. PLoS One. 2013 8:e53790. PMCID PMC3547069.

Verrier JD, Jackson TC, Gillespie DG, Janesko-Feldman K, Bansal R, Nave AK, Kochanek PM, Jackson EK. Role of CNPase in the oligodendrocytic extracellular 2',3'-cAMP-adenosine pathway. GLIA. 2013, 61:1595-1606. PMCID PMC3998092

Wang Z, Subramanya A, Satlin LM, Pastor-Soler N, Carattino M, and TR Kleyman. Regulation of large conductance Ca2+- activated K+ channels by WNK4 kinase. Am J Physiol Cell Physiol. 2013, 305:C846-53, 2013. PMCID PMC3798677

Yoshida, O., Kimura, S., Jackson, E.K., Robson, S.C., Geller, D.A., Murase, N., and Thomson, A.W.: CD39 expression by hepatic myeloid dendritic cells attenuates inflammation in liver transplant ischemia-reperfusion injury. Hepatology 2013, 58: 2163-2175. PMCID: 3844081

Youker RT, Bruns JR, Costa SA, Rbaibi Y, Lanni F, Kashlan OB, Teng H, Weisz OA. Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization. Mol Biol Cell. 2013, 24:1996-2007. PMCID PMC3681702.



2014 

Publications 2014:

Al-Bataineh MM, Gong F, Marciszyn A, Myerburg MM, Pastor-Soler NM. Regulation of the Proximal Tubule Vacuolar H+-ATPase by PKA and AMP-Activated Protein Kinase. Am J Physiol Renal Physiol. 2014, 306:F981-95. PMCID PMC4010682

Apodaca G, Brown WJ. Membrane traffic research: challenges for the next decade. Front Cell Dev Biol. 2014 Sep 17;2:52. doi: 10.3389/fcell.2014.00052. eCollection 2014. PMCID PMC4207031.

Brodsky JL, Clark PL. Protein folding in the cell, from atom to organism. FASEB J. 2014 Dec;28(12):5034-8. PMID: 25451678 PMCID Exempt

Carattino MD, Mueller GM, Palmer LG, Frindt G, Rued AC, Hughey RP, Kleyman TR. Prostasin interacts with the epithelial Na+ channel and facilitates cleavage of the γ-subunit by a second protease. Am J Physiol Renal Physiol. 2014 Nov 1;307(9):F1080-7. PMCID PMC4216988

Carrisoza-Gaytán R, Liu Y, Flores D, Else D, Lee HG, Rhodes G, Sandoval R, Kleyman T, Lee F, Molitoris B, Satlin LM, Rohatgi R. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). Am J Physiol Renal Physiol 307:F195-204, 2014. PMCID PMC4152160

Chen J, Kleyman TR, Sheng S. Deletion of α-subunit exon 11 of the epithelial Na+ channel reveals a regulatory module. Am J Physiol Renal Physiol. 2014, 306:F561-7. PMCID PMC3949035

Cirio MC, de Groh ED, de Caestecker MP, Davidson AJ, Hukriede NA. Kidney regeneration: Common themes from the embryo to the adult. Pediatric Nephrology 2014, 29:553-64. PMCID: PMC3944192

Edinger RS, Coronnello C, Bodnar AJ, Laframboise WA, Benos PV, Ho J, Johnson JP, Butterworth MB. Aldosterone Regulates MicroRNAs in the Cortical Collecting Duct to Alter Sodium Transport. J Am Soc Nephrol. 2014, Nov;25(11):2445-57. PMCID PMC4214524.

Gallo LI, Liao Y, Ruiz WG, Clayton DR, Li M, Liu YJ, Jiang Y, Fukuda M, Apodaca G, Yin XM. TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. Mol Biol Cell. 2014 Nov 15;25(23):3779-97. PMCID PMC4230784.

Gidon A, Al-Bataineh MM, Jean-Alphonse FG, Stevenson HP, Watanabe T, Louet C, Khatri A, Calero G, Pastor-Soler NM, Gardella TJ, Vilardaga JP. Endosomal GPCR signaling turned off by negative feedback actions of PKA and v-ATPase. Nat Chem Biol. 2014 Sep;10(9):707-9. PMCID PMC4138287.

Hatfield SM, Kjaergaard J, Lukashev D, Belikoff B, Schreiber TH, Sethumadhavan S, Abbott R, Philbrook P, Thayer M, Shujia D, Rodig S, Kutok JL, Ren J, Ohta A, Podack ER, Karger B, Jackson EK, Sitkovsky M. Systemic oxygenation weakens the hypoxia and hypoxia inducible factor 1α-dependent and extracellular adenosine-mediated tumor protection. J Mol Med. 2014, 92: 1283-1292. PMCID PMC4247798

Hecht KA, O'Donnell AF, Brodsky JL. The proteolytic landscape of the yeast vacuole. Cell Logist. 2014, Jan 1;4(1):e28023. PMCID PMC4022603

Ireland AW, Gobillot TA, Gupta T, Seguin SP, Liang M, Resnick L, Goldberg MT, Manos-Turvey A, Pipas JM, Wipf P, Brodsky JL. Synthesis and structure–activity relationships of small molecule inhibitors of the simian virus 40 T antigen oncoprotein, an anti-polyomaviral target. Bioorg Med Chem. 2014, Nov 15;22:6490-502. PMCID PMC4293281

Jackson EK, Cheng D, Mi Z, Gillespie DG. Guanosine regulates adenosine levels in the kidney. Physiol Rep. 2014, 2: e12028. PMCID PMC4098751

Jackson EK, Cheng D, Verrier JD, Janesko-Feldman K, Kochanek PM. Interactive roles of CD73 and tissue non-specific alkaline phosphatase in the renal vascular metabolism of 5’-AMP. Am J Physiol Renal Physiol. 2014, 307: F680-F685. PMCID PMC4166734

Jackson EK, Gillespie DG, Mi Z, Cheng D, Bansal R, Janesko-Feldman K, Kochanek PM. Role of 2’,3’-cyclic nucleotide 3’-phosphodiesterase in the renal 2’,3’-cAMP-adenosine pathway. Am J Physiol Renal Physiol. 2014, 307: F14-F24. PMCID PMC4080157

Jackson EK, Mi Z. The guanosine-adenosine interaction exists in vivo. J Pharmacol Exp Ther. 2014, 350: 719-726. PMCID PMC4152880

Kleyman, TR and Myerburg MM. Proteases, ENaCs and cystic fibrosis. J Physiol. 2014, Dec 1;592(Pt 23):5145. PMCID PMC4262328.

Kolb AR, Needham PG, Rothenberg C, Guerriero CJ, Welling PA, Brodsky JL. ESCRT regulates surface expression of the Kir2.1 potassium channel. Mol Biol Cell. 2014, 25:276-89. PMCID PMC3890348

Koral K, Li H, Ganesh N, Birnbaum MJ, Hallows KR, Erkan E. Akt recruits Dab2 to albumin endocytosis in the proximal tubule. Am J Physiol Renal Physiol. 2014, Dec 15;307(12):F1380-9. PMCID PMC4269696

Labilloy A, Youker RT, Bruns JR, Kukic I, Kiselyov K, Halfter W, Finegold D, do Monte SJ, Weisz OA. Altered dynamics of a lipid raft associated protein in a kidney model of Fabry disease. Mol Genet Metab. 2014, 111:184-92. PMCID PMC3946758

Mattila PE, Raghavan V, Rbaibi Y, Baty CJ, and Weisz OA. Rab11a-positive compartments in proximal tubule cells sort fluid phase and membrane cargo. Am J Physiol Cell Physiol. 2014, 306:C441-449. PMCID PMC4042618

Morrell ED, Kellum JA, Hallows KR, Pastor-Soler NM. Epithelial transport during septic acute kidney injury. Nephrol Dial Transplant. 2014, Jul;29(7):1312-9. PMCID PMC4071050

Mukherjee A, Mueller GM, Kinlough CL, Sheng N, Wang Z, Mustafa SA, Kashlan OB, Kleyman TR, Hughey RP. Cys-Palmitoylation of the Gamma Subunit has a Dominant Role in Modulating Activity of the Epithelial Sodium Channel. J Biol Chem. 2014, 289: 14351-14359. PMCID PMC4022901

Novitskaya T, McDermott L, Zhang KX, Chiba T, Paueksakon P, Hukriede NA, de Caestecker MP. A PTBA class small molecule enhances recovery and reduces post injury fibrosis after aristolochic acid-induced kidney injury. Am J Physiol Renal Physiol. 2014, 306: F496-504. PMCID PMC3949031

Prakasam HS, Gallo LI, Li H, Ruiz WG, Hallows KR, Apodaca G. A1 adenosine receptor-stimulated exocytosis in bladder umbrella cells requires phosphorylation of ADAM17 Ser-811 and EGF receptor transactivation. Mol Biol Cell. 2014, Nov 15;25(23):3798-812. PMCID PMC4230785.

Raghavan V, Rbaibi R, Pastor-Soler N, Carattino MD, and Weisz OA. Shear stress dependent regulation of apical endocytosis in renal proximal tubule cells mediated by primary cilia. Proc Natl Acad Sci USA. 2014, 111:8506-8511. PMCID PMC4060694.

Schuler PJ, Saze Z, Hong CS, Muller L, Gillespie DG, Cheng D, Harasymczuk M, Mandapathil M, Lang S, Jackson EK, Whiteside TL. Human CD4+CD39+ regulatory T cells produce adenosine upon co-expression of surface CD73 or contact with CD73+ exosomes or CD73+ cells. Clin Exp Immunol. 2014, 177: 531-543. PMCID PMC4226604

Surlow BA, Cooley BM, Needham PG, Brodsky JL, Patton-Vogt J. Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation. J Biol Chem. 2014, Nov 7;289(45):31591-604. PMCID PMC4223355

Szalinski CM, Labilloy A, Bruns JR, Weisz OA. VAMP7 modulates ciliary biogenesis in kidney cells. PLoS One. 2014, 9:e86425. PMCID PMC3899255

Tomar R, Mudumana S, Pathak N, Hukriede NA, Drummond IA. osr1 is required for podocyte development downstream of wt1. J Am Soc Nephrol. 2014, Nov;25(11):2539-45. In press. PMCID PMC4214536

Warnock DG, Kusche-Vihrog K, Tarjus A, Sheng S, Oberleithner H, Kleyman TR, Jaisser F. Blood pressure and amiloride-sensitive Na+ channels in vascular and renal cells. Nat. Rev. Nephrol. 2014, Mar;10(3):146-57. PMCID PMC4137491

Wei Y, Liao Y, Zavilowitz B, Ren J, Liu W, Chan P, Rohatgi R, Estilo G, Jackson EK, Wang W, Satlin LM. Angiotensin type 2 receptor regulates ROMK-like K channel activity in renal cortical collecting duct during high dietary potassium adaptation. Am J Physiol Renal Physiol. 2014, 307:F833-43. PMCID PMC4187043

Yao M, Rogers NM, Csanyi G, Rodriguez AI, Ross MA, St. Croix C, Thomson AW, Pagano PJ, Isenberg JS. Matricellular Activation of Non-Phagocytic SIRP-α Stimulates NADPH Oxidase to Control Vascular Tone, Limit Blood Flow and Promote Ischemia Reperfusion Injury. J Am Soc Nephrol. 2014, Jun;25(6):1171-86. PMCID PMC4033366

2015 

Publications 2015:

An P, Brodsky JL, Pipas JM. The conserved core enzymatic activities and the distinct dynamics of polyomavirus large T antigens. Arch Biochem Biophys. 2015 May 1;573:23-31. PMCID PMC4865250

Brodsky JL, Frizzell RA. A combination therapy for cystic fibrosis. Cell. 2015 Sep 24;163(1):17. PubMed PMID: 26406363. PMCID PMC4865247

Buck TM, Brodsky JL. Escaping the endoplasmic reticulum: why does a molecular chaperone leave home for greener pastures? EMBO J. 2015 Jan 2;34(1):1-3. PMCID PMC4291476

Buck TM, Jordan R, Lyons-Weiler J, Adelman JL, Needham PG, Kleyman TR, Brodsky JL. Expression of three topologically distinct membrane proteins elicits unique stress response pathways in the yeast Saccharomyces cerevisiae. Physiol Genomics. 2015 Jun;47(6):198-214. PMID: 25759377; PMCID PMC4451388.

Chang A, Yeung S, Thakkar A, Kevin M, Huang KM, Liu, MM, Kanassatega RS, Parsa C, Orlando R., Jackson EK, Andresen BT, Huang Y. Prevention of skin carcinogenesis by the -blocker carvedilol. Cancer Prev Res. 2015, 8:27-36. PMCID PMC4289657

Chen J, Ray EC, Yates ME, Buck TM, Brodsky JL, Kinlough CL, Winarski KL, Hughey RP, Kleyman TR, Sheng S. Functional Roles of Clusters of Hydrophobic and Polar Residues in the Epithelial Na+ Channel Knuckle Domain. J Biol Chem. 2015, Oct 9;290(41):25140-50. PMCID PMC4599017.

Crum TS, Gleixner AM, Posimo JM, Mason DM, Broeren MT, Heinemann SD, Wipf P, Brodsky JL, Leak RK. Heat shock protein responses to aging and proteotoxicity in the olfactory bulb. J Neurochem. 2015 Jun;133(6):780-94. PMCID PMC4464935

Di Giovanni V, Walker KA, Bushnell D, Schaefer C, Sims-Lucas S, Puri P, Bates CM. Fibroblast growth factor receptor-Frs2α signaling is critical for nephron progenitors. Dev Biol. 2015, Apr 1;400(1):82-93. PMCID PMC4361332

Dubey RK, Finderle J, Gillespie DG, Mi Z, Rosselli M, Imthurn B, Jackson EK. Adenosine attenuates human coronary artery smooth muscle cell proliferation by inhibiting multiple signaling pathways that converge on cyclin D. Hypertension 2015, 66: 1207-1219. PMCID PMC4644125

Hatfield SM, Kjaergaard J., Lukashev D, Schreiber TH, Belikoff B, Abbott R, Sethumadhavan S, Philbrook P, Ko K, Cannici R, Thayer M, Rodig S, Kutok JL, Jackson EK, Karger B, Podack ER, Ohta A, Sitkovsky M. Immunological mechanisms of the antitumor effects of supplemental oxygenation. Science Translational Medicine 2015, 7: 277ra30. PMCID PMC4641038

He T, Brocca-Cofano E, Gillespie DG, Xu C, Stock JL, Ma G, Policicchio BB, Raehtz KD, Rinaldo CR, Apetrei C, Jackson EK, Macatangay BJC, Pandrea I. Critical role for the adenosine pathway in controlling SIV-related immune activation and inflammation in gut mucosal tissues. J Virol 2015, 89: 9616-9630. PMCID PMC4542384

Heidrich E, Carattino MD, Hughey RP, Pilewski JM, Kleyman TR, Myerburg MM. Intracellular Na+ regulates epithelial Na+ channel maturation. J Biol Chem. 2015, May 1;290(18):11569-77. PMCID PMC4416860

Jackson EK, Mi Z, Tofovic SP, Gillespie DG. Effect of dipeptidyl peptidase 4 inhibition on arterial blood pressure is context dependent. Hypertension 2015, 65: 238-249. PMCID PMC4268428

Jiang C, Veon W, Li H, Hallows KR, Roy P. Epithelial morphological reversion drives Profilin-1-induced elevation of p27(kip1) in mesenchymal triple-negative human breast cancer cells through AMP-activated protein kinase activation. Cell Cycle. 2015, 14(18):2914-23. PMCID PMC4825560

Kashlan OB, Blobner BM, Zuzek Z, Tolino M, Kleyman TR. Na+ inhibits the epithelial Na+ channel by binding to a site in an extracellular acidic cleft. J Biol Chem 2015, 290(1):568-76. PMCID PMC4281758

Kullmann FA, Birder LA, Andersson KE, Translational research and functional changes in voiding function in older adults. Clin Geriatr Med. 2015, Nov;31(4):535-48. PMCID PMC4865381

Li H, Satriano J, Thomas JL, Miyamoto S, Sharma K, Pastor-Soler NM, Hallows KR, Singh P. Interactions between HIF-1α and AMPK in the regulation of cellular hypoxia adaptation in chronic kidney disease. Am J Physiol Renal Physiol. 2015, Sep 1;309(5):F414-28. PMCID PMC4556888.

Marcoline FV, Bethel N, Guerriero CJ, Brodsky JL, Grabe M. Membrane Protein Properties Revealed through Data-Rich Electrostatics Calculations. Structure. 2015, Aug 4;23(8):1526-37. PMCID PMC4527966.

Miller RL, Denny GO, Knuepfer MM, Kleyman TR, Jackson EK, Salkoff LB, Loewy AD. Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema. Brain Research. 1601: 40-51, 2015. PMCID PMC4346418

Montalbetti N, Rued AC, Clayton DR, Ruiz WG, Bastacky SI, Prakasam HS, Eaton AF, Kullmann FA, Apodaca G, Carattino MD. Increased urothelial paracellular transport promotes cystitis. Am J Physiol Renal Physiol. 2015, Dec 15;309(12):F1070-81. PMCID PMC4683306.

Needham PG, Patel HJ, Chiosis G, Thibodeau PH, Brodsky JL. Mutations in the Yeast Hsp70, Ssa1, at P417 Alter ATP Cycling, Interdomain Coupling, and Specific Chaperone Functions. J Mol Biol. 2015, Sep 11;427(18):2948-65. PMCID PMC4569534

Newell EA, Exo JL, Verrier JD, Jackson TC, Gillespie DG, Janesko-Feldman K, Kochanek PM, Jackson EK. 2',3'-cAMP, 3'-AMP, 2'-AMP and adenosine inhibit TNF-α and CXCL10 production from activated primary murine microglia via A2A receptors. Brain Research 2015, 1594: 27-35. PMCID PMC4262711

Pastor-Soler NM, Sutton TA, Mang HE, Kinlough CL, Gendler SJ, Madsen CS, Bastacky SI, Ho J, Al-Bataineh MM, Hallows KR, Singh S, Monga SP, Kobayashi H, Haase VH, Hughey RP. Muc1 is Protective during Kidney Ischemia-Reperfusion Injury. Am J Physiol Renal Physiol. 2015, Jun 15;308(12):F1452-62. PMCID PMC4469889

Posimo JM, Weilnau JN, Gleixner AM, Broeren MT, Weiland NL, Brodsky JL, Wipf P, Leak RK. Heat shock protein defenses in the neocortex and allocortex of the telencephalon. Neurobiol Aging. 2015, May;36(5):1924-37. PMCID PMC4417007

Pradhan D, Roy S, Quiroga-Garza G, Cieply K, Mahaffey AL, Bastacky S, Dhir R, Parwani AV. Validation and utilization of a TFE3 break-apart FISH assay for Xp11.2 translocation renal cell carcinoma and alveolar soft part sarcoma. Diagn Pathol. 2015, Sep 29;10:179. PMCID PMC4587681.

Priedigkeit NM, Wolfe NW, Clark NL. Evolutionary signatures amongst disease genes permit novel methods for gene prioritization and construction of informative gene networks. PLOS Genetics. 2015, 11(2): e1004967. PMCID PMC4334549.

Prosser DC, Pannunzio AE, Brodsky JL, Thorner J, Wendland B, O'Donnell AF. α-Arrestins participate in cargo selection for both clathrin-independent and clathrin-mediated endocytosis. J Cell Sci. 2015, Nov 15;128(22):4220-34. PMCID PMC4712785.

Qian X, Li X, Ilori TO, Klein JD, Hughey RP, Li CJ, Alli AA, Guo Z, Yu P, Song X, Chen G. RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla. Front Physiol. 2015, Oct 1;6:274. PMCID PMC4590316

Rahaman MM, Reinders FG, Koes D, Nguyen AT, Mutchler SM, Sparacino-Watckins C, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ, Straub AC. Structure guided chemical modifications of propylthiouracil reveal novel small molecule inhibitors of cytochrome b5 reductase 3 that increase nitric oxide bioavailability. J Biol Chem. 2015, 290: 16861-16872. PMCID PMC4505432

Ray EC, Kleyman TR. Cutting it out: ENaC processing in the human nephron. J Am Soc Nephrol. 2015, 26:1-3. PMCID: PMC4279749.

Ray EC, Rondon-Berrios H, Boyd CR, Kleyman TR. Mechanisms of sodium retention and volume expansion in nephrotic syndrome: implications for hypertension. Adv. Chronic Kidney Dis. 2015, 22:179-184. PMCID PMC4409655

Reay DP, Bastacky SI, Wack KE, Stolz DB, Robbins PD, Clemens PR. D-Amino Acid Substitution of Peptide-Mediated NF-κB Suppression in mdx Mice Preserves Therapeutic Benefit in Skeletal Muscle, but Causes Kidney Toxicity. Mol Med. 2015, PMCID PMC4559531.

Rigassi L, Bozzolo FB, Lucchinetti E, Zaugg M, Fingerle F, Rosselli M, Imthurn B, Jackson EK, Dubey RK. 2-Methoxyestradiol blocks the RhoA/ROCK1 pathway in human aortic smooth muscle cells. Am J Physiol Endocrinol Metab 2015, 309: E995-E1007. PMCID PMC4816197

Roy A, Al-Bataineh MM, Pastor-Soler NM. Collecting duct intercalated cell function and regulation. Clin J Am Soc Nephrol. 2015, Feb 6;10(2):305-24. PMCID PMC4317747.

Roy A, Al-Qusairi L, Donnelly BF, Ronzaud C, Marciszyn AL, Gong F, Chang YP, Butterworth MB, Pastor-Soler NM, Hallows KR, Staub O, Subramanya AR. Alternatively spliced proline-rich cassettes link WNK1 to aldosterone action. J Clin Invest. 2015 Sep;125(9):3433-48. PMCID PMC4588284

Roy A, Goodman JH, Begum G, Donnelly BF, Pittman G, Weinman EJ, Sun D, Subramanya AR. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol. 2015, Feb 15;308(4):F366-76. PMCID PMC4329490.

Stolz DB, Sims-Lucas S. Unwrapping the origins and roles of the renal endothelium. Pediatr Nephrol. 2015, Jun;30(6):865-72. PMCID PMC4164630.

Tan RJ, Zhou D, Xiao L, Zhou L, Li Y, Bastacky SI, Oury TD, Liu Y. Extracellular Superoxide Dismutase Protects against Proteinuric Kidney Disease. J Am Soc Nephrol. 2015, Oct;26(10):2447-59. PMCID PMC4587687

Verrier JD, Kochanek PM, Jackson EK. Schwann cells metabolize extracellular 2’,3’-cAMP to 2’-AMP. J Pharmacol Exp Ther. 354: 175-183, 2015. PMCID PMC4518068

Wipf P, Eyer BR, Yamaguchi Y, Zhang F, Neal MD, Sodhi CP, Good M, Branca M, Prindle T Jr, Lu P, Brodsky JL, Hackam DJ. Synthesis of anti-inflammatory α-and β-linked acetamidopyranosides as inhibitors of toll-like receptor 4 (TLR4). Tetrahedron Lett. 2015, Jun3;56(23):3097-3100. PMCID PMC4518473.

Wolfe NW and Clark NL. ERC Analysis: web-based inference of gene function via Evolutionary Rate Covariation. Bioinformatics. 2015, 31(23):3835-7. PMCID PMC4751245.

Zhu X, Gillespie DG, Jackson EK. NPY1-36 and PYY1-36 activate cardiac fibroblasts: An effect enhanced by genetic hypertension and inhibition of dipeptidyl peptidase 4. Am J Physiol Heart Circ Physiol 2015, 309:H1528-H1542. PMCID PMC4666977

2016 

Publications 2016:

Al-Bataineh MM, Alzamora R, Ohmi K, Ho PY, Marciszyn AL, Gong F, Li H, Hallows KR, Pastor-Soler NM. Aurora Kinase A Activates the Vacuolar H+-ATPase (V-ATPase) in Kidney Carcinoma Cells. Am J Physiol Renal Physiol. 2016, Jun 1;310(11):F1216-28. PMCID PMC4935778

Al-Bataineh MM, Kinlough CL, Poland PA, Pastor-Soler NM, Sutton TA, Mang HE, Bastacky SI, Gendler SJ, Madsen CS, Singh S, Monga SP, Hughey RP. Muc1 enhances the β-catenin protective pathway during ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2016, Mar 15;310(6):F569-79. PMCID PMC4796271.

Al-Bataineh MM, Li H, Ohmi K, Gong F, Marciszyn AL, Naveed S, Zhu X, Neumann D, Wu Q, Cheng L, Fenton RA, Pastor-Soler NM, Hallows KR. Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F890-F900. PMCID PMC5533229

Al-Qusairi L, Basquin D, Roy A, Stifanelli M, Rajaram RD, Debonneville A, Nita I, Maillard MP, Loffing J, Subramanya AR, Staub O. Renal-tubular SGK1 deficiency causes impaired K+ excretion via the loss of regulation of NEDD4-2/WNK1 and ENaC. Am J Physiol Renal Physiol. 2016, Aug 1;311(2):F330-42. PMCID PMC5005279

Bebee TW, Sims-Lucas S, Park JW, Bushnell D, Cieply B, Xing Y, Bates CM, Carstens RP. Ablation of the epithelial-specific splicing factor Esrp1 results in ureteric branching defects and reduced nephron number. Dev Dyn. 2016 Oct;245(10):991-1000. PMCID PMC5096029.

Bhuiyan MI, Song S, Yuan H, Begum G, Kofler J, Kahle KT, Yang SS, Lin SH, Alper SL, Subramanya AR, Sun D. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab. 2016 Aug;37(8):2780-2794. PMCID PMC5536788

Carrisoza-Gaytan R, Carattino MD, Kleyman TR, Satlin LM. An unexpected journey: conceptual evolution of mechanoregulated potassium transport in the distal nephron Am J Physiol Cell Physiol. 2016 Feb 15;310(4):C243-59. PMCID PMC4838068

Chiba T, Skrypnyk NI, Skvarca LB, Penchev R, Zhang KX, Rochon ER, Fall JL, Paueksakon P, Yang H, Alford CE, Roman BL, Zhang MZ, Harris R, Hukriede NA, de Caestecker MP. Retinoic Acid Signaling Coordinates Macrophage-Dependent Injury and Repair after AKI. J Am Soc Nephrol. 2016, 27:495-508. PMCID: PMC4731115

Christensen M, Jensen JB, Jakobsen S, Jessen N, Frøkiær J, Kemp BE, Marciszyn AL, Li H, Pastor-Soler NM, Hallows KR, Nørregaard R. Renoprotective Effects of Metformin are Independent of Organic Cation Transporters 1 & 2 and AMP-activated Protein Kinase in the Kidney. Sci Rep. 2016, Oct 26;6:35952. PMCID PMC5080611

Chung WJ, Goeckeler-Fried JL, Havasi V, Chiang A, Rowe SM, Plyler ZE, Hong JS, Mazur M, Piazza GA, Keeton AB, White EL, Rasmussen L, Weissman AM, Denny RA, Brodsky JL, Sorscher EJ. Increasing the Endoplasmic Reticulum Pool of the F508del Allele of the Cystic Fibrosis Transmembrane Conductance Regulator Leads to Greater Folding Correction by Small Molecule Therapeutics. PLoS One. 2016, Oct 12;11(10):e0163615. PMCID PMC5061379

Figueiró F, Muller L., Funk, S, Jackson, EK, Battastini, AMO, Whiteside, T.L.: Phenotypic and functional characteristics of CD39high human regulatory B cells. OncoImmunology 5: e1082703, 2016. PMCID PMC4801473

Hao S, DelliPizzi A, Quiroz-Munoz M, Jiang H, Ferreri N. The EP3 receptor regulates water excretion in response to high salt intake. Am J Physiol Renal Physiol. 2016, Oct 1;311(4):F822-F829. PMCID PMC5142234

Jackson EK, Gillespie DG, Mi Z. 8-Aminoguanosine and 8-aminoguanine exert diuretic, natriuretic, glucosuric and antihypertensive activity. J Pharmacol Exp Ther. 2016, 359: 420-435. PMCID PMC5118650

Jackson EK, Menshikova EV, Mi Z, Verrier JD, Banal R, Janesko-Feldman K, Jackson TC, Kochanek PM. Renal 2’,3’-cyclic nucleotide 3’-phosphodiesterase is an important determinant of AKI severity after ischemia-reperfusion. J Am Soc Nephrol. 2016, Jul;27(7):2069-81. PMCID PMC4926972

Kanai AJ, Birder LA, Ikeda Y, Kullmann FA, Fry C. Implications for bidirectional signaling between afferent nerves and urothelial cells. Neurourol Urodyn. 2016, Feb;35(2):273-7. PMCID PMC4865379

Kharade SV, Flores D, Lindsley CW, Satlin LM, and JS Denton. ROMK inhibitor actions in the nephron probed with diuretics. Am J Physiol Renal Physiol. 2016, Apr 15;310(8):F732-F737. PMCID PMC4835929

Khurshid K, Yabes J, Weiss PM, Dharia S, Brown L, Unruh M, Jhamb M. Effect of antihypertensive medications on the severity of obstructive sleep apnea: A systematic review and meta-analysis. J Clin Sleep Med. 2016, Aug 15;12(8):1143-51. PMCID PMC4957192

Krauson AJ, Carattino MD. Thumb domain mediates acid-sensing ion channel desensitization. J Biol Chem. 2016, May 20;291(21):11407-19. PMCID PMC4900284

Manos-Turvey A, Al-Ashtal HA, Needham PG, Hartline CB, Prichard MN, Wipf P, Brodsky JL. Dihydropyrimidinones and -thiones with improved activity against human polyomavirus family members. Bioorg Med Chem Lett. 2016, Oct 15;26(20):5087-5091. PMCID PMC5050167

Maringer K, Sims-Lucas S. The multifaceted role of the renal microvasculature during acute kidney injury. Pediatr Nephrol. 2016, August;31(8):1231-40. PMCID PMC4841763.

McClure ML, Barnes S, Brodsky JL, Sorscher EJ. Trafficking and function of the cystic fibrosis transmembrane conductance regulator: a complex network of posttranslational modifications. Am J Physiol Lung Cell Mol Physiol. 2016, Oct 1;311(4):L719-L733. PMCID PMC5142128

Moulton DE, Sulzer V, Apodaca G, Byrne HM, Waters SL. Mathematical modelling of stretch-induced membrane traffic in bladder umbrella cells. J Theor Biol. 2016, Nov 21;409:115-132. PMCID: PMC5478889

Naylor RW, Skvarca LB, Thisse C, Thisse B, Hukriede NA, Davidson AJ. BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis. Nat Commun. 2016, Jul 13;7:12197. PMCID PMC4947171.

Nizar JM, Dong W, McClellan RB, Labarca M, Zhou Z, Wong J, Goens DG, Zhao M, Velarde N, Bernstein D, Pellizon M, Satlin LM, Bhalla V. Na+-sensitive elevation in blood pressure is ENaC independent in diet-induced obesity and insulin resistance. Am J Physiol Renal Physiol. 2016, May 1;310(9):F812-20. PMCID PMC4867314

Pereira EM, Labilloy A, Eshbach ML, Roy A, Subramanya AR, Monte S, Labilloy G, Weisz OA. Characterization and phosphoproteomic analysis of a human immortalized podocyte model of Fabry disease generated using CRISPR/Cas9 technology. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F1015-F1024. PMCID PMC5130460.

Puri P, Bushnell D, Schaefer CM, Bates CM. Six2creFrs2α knockout mice are a novel model of renal cystogenesis. Sci Rep. 2016, Nov 17;6:36736. PMCID PMC5113122.

Raghavan V, Weisz OA. Discerning the role of mechanosensors in regulating proximal tubule function. Am J Physiol Renal Physiol. 2016, Jan 1;310(1):F1-5. PMCID PMC4675802

Ramani K, Garg, AV, Jawale CV, Conti HR, Whibley N, Jackson EK, Shiva SS, Kolls JK, Gaffen SL, Biswas PS. The kallikrein-kinin system: A novel mediator of IL-17-driven anti-candida immunity in the kidney. PLOS Pathogens 2016, 22: e1005952. PMCID PMC5096720

Ray EC, Boyd-Shiwarski C, Kleyman TR. Why diuretics fail failing hearts. J Am Soc Nephrol. 2017, In Press. PMCID In process

Ray EC, Chen J, Kelly TN, He J, Hamm LL, Gu D, Shimmin LC, Hixson JE, Rao DC, Sheng S, Kleyman TR. Human epithelial Na+ channel missense variants identified in the GenSalt study alter channel activity. Am J Physiol Renal Physiol. 2016, Nov 1;311(5):F908-F914. PMCID PMC5130461

Sabnis AJ, Guerriero CJ, Olivas V, Sayana A, Shue J, Flanagan J, Asthana S, Paton AW, Paton JC, Gestwicki JE, Walter P, Weissman JS, Wipf P, Brodsky JL, Bivona TG. Combined chemical-genetic approach identifies cytosolic HSP70 dependence in rhabdomyosarcoma. Proc Natl Acad Sci USA. 2016, Aug 9;113(32):9015-20. PMCID PMC4987817

Schaufelberger SA, Rosselli M, Barchiesi F, Gillespie DG, Jackson EK, Dubey RK. 2-Methoxyestradiol, an endogenous 17-estradiol metabolite, inhibits microglial proliferation and activation via an estrogen receptor-independent mechanism. Am J Physiol Endocrinol Metab. 2016, 310:E313-322. PMCID PMC4773653

Sharma A, Yabes J, Al Mawed S, Wu C, Stilley C, Unruh M, Jhamb M. Impact of Cognitive Function Change on Mortality in Renal Transplant and End-Stage Renal Disease Patients. Am J Nephrol. 2016, 44(6):462-472. PMCID PMC5143182.

Shi S, Luke CJ,  Miedel MT, Silverman GA, Kleyman TR. Activation of the Caenorhabditis elegans degenerin channel by shear stress requires the MEC-10 subunit. J. Biol. Chem. 2016, Jul 1;291(27):14012-22. PMCID PMC4933161

Skrypnyk NI, Sanker S, Brilli-Skvarca L, Novitskaya T, Woods C, Chiba T, Patel K, Goldberg ND, McDermott L, Vinson PN, Calcutt MW, Huryn DM, Vernetti LA, Vogt A, Hukriede N, de Caestecker MP. Delayed treatment with PTBA analogs reduces post injury renal fibrosis after kidney injury. Am J Physiol Renal Physiol. 2016, Apr 15;310(8):F705-F716. PMCID PMC4835925.

Sneddon WB, Ruiz GW, Gallo LI, Xiao K, Zhang Q, Rbaibi Y, Weisz OA, Apodaca GL, Friedman PA. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport. J Biol Chem. 2016, Sep 2;291(36):18632-42. PMCID PMC5009241.

Song R, Preston G, Kidd L, Bushnell D, Sims-Lucas S, Bates CM, Yosypiv IV. Prorenin receptor is critical for nephron progenitors. Dev Biol. 2016, Jan 15;409(2):382-91. PMCID PMC4724493.

Tan RJ, Chartoumpekis DV, Rush BM, Zhou D, Fu H, Kensler TW, Liu Y. Keap1 hypomorphism protects against ischemic and obstructive kidney disease. Sci Rep. 2016, Nov 2;6:36185. PMCID PMC5090361.

Tofovic SP, Eman M, Salah EM, Smits GJ, Whalley ET, Ticho B, Deykin A, Jackson EK. Dual A1/A2B receptor blockade improves cardiac and renal outcomes in a rat model of heart failure with preserved ejection fraction. J Pharmacol Exp Ther. 2016, 356: 333-340. PMCID PMC4727158

Veit G, Avramescu RG, Chiang AN, Houck SA, Cai Z, Peters KW, Hong JS, Pollard HB, Guggino WB, Balch WE, Skach WR, Cutting GR, Frizzell RA, Sheppard DN, Cyr DM, Sorscher EJ, Brodsky JL, Lukacs GL. From CFTR biology toward combinatorial pharmacotherapy: expanded classification of cystic fibrosis mutations. Mol Biol Cell. 2016, Feb 1;27(3):424-33. PMCID PMC4751594

Wagner MC, Campos-Bilderback SB, Chowdhury M, Flores B, Lai X, Myslinski J, Pandit S, Sandoval RM, Wean SE, Satlin LM, Wiggins RC, Witzmann FA, Molitoris BA. Proximal tubules have the capacity to regulate uptake of albumin. J Am Soc Nephrol. 2016, Feb;27(2):482-94. PMCID PMC4731114

Walker KA, Sims-Lucas S, Bates CM. Fibroblast growth factor receptor signaling in kidney and lower urinary tract development. Pediatr Nephrol. 2016, Jun;31(6):885-95. PMCID PMC4761523.

Webb TN, Carrisoza-Gaytan R, Montalbetti N, Rued A, Roy A, Socovich AM, Subramanya AR, Satlin LM, Kleyman TR, Carattino MD. Cell-specific regulation of L-WNK1 by dietary K. Am J Physiol Renal Physiol. 2016, Jan 1;310(1):F15-26. PMCID PMC4675801.

Zhang Q, Dai H, Yatim KM, Abou-Daya K, Williams AL, Oberbarnscheidt MH, Camirand G, Rudd CE, Lakkis FG. CD8+ Effector T Cell Migration to Pancreatic Islet Grafts Is Dependent on Cognate Antigen Presentation by Donor Graft Cells. J Immunol. 2016 Aug 15;197(4):1471-6. PMCID PMC5023061.

Zhuang Q, Liu Q, Divito SJ, Zeng Q, Yatim KM, Hughes AD, Rojas-Canales DM, Nakao A, Shufesky WJ, Williams AL, Humar R, Hoffman RA, Shlomchik WD, Oberbarnscheidt MH, Lakkis FG, Morelli AE. Graft-infiltrating host dendritic cells play a key role in organ transplant rejection. Nat Commun. 2016, Aug 24;7:12623. PMCID PMC4999515.


2017 

Publications 2017:

Agrawal PB, Wang R, Li HL, Schmitz-Abe K, Simone-Roach C, Chen J, Shi J, Louie T, Sheng S, Towne MC, Brainson CF, Matthay MA, Kim CF, Bamshad M, Emond MJ, Gerard NP, Kleyman TR, Gerard C. Epithelial Sodium Channel ENaC is a modifier of the long term non-progressive phenotype associated with F508del CFTR mutations. Am. J. Respir. Cell Mol. Biol. 2017, In press. PMCID In process

Al-Qusairi L, Basquin D, Roy A, Rajaram RD, Maillard MP, Subramanya AR, Staub O. Renal Tubular Ubiquitin-Protein Ligase NEDD4-2 Is Required for Renal Adaptation during Long-Term Potassium Depletion. J Am Soc Nephrol. 2017, Aug;28(8):2431-2442. PMCID PMC28289184

Apodaca G. Role of Polarity Proteins in the Generation and Organization of Apical Surface Protrusions. Cold Spring Harb Perspect Biol. 2017 Mar 6. pii: a027813. In press. PMCID In process.

Bhattacharyya S, Jean-Alphonse FG, Raghavan V, McGarvey JC, Rbaibi Y, Vilardaga JP, Carattino MD, Weisz OA. Cdc42 activation couples fluid shear stress to apical endocytosis in proximal tubule cells. Physiol Rep. 2017 Oct;5(19). pii: e13460. PMCID: PMC5641940.

Boyd-Shiwarski CR, Shiwarski DJ, Roy A, Nkashama LJ, Namboodiri HN, Xie J, McClain KL, Marciszyn A, Kleyman TR, Tan RJ, Stolz DB, Puthenveedu MA, Huang CL, Subramanya AR. Potassium-Regulated Distal Tubule WNK Bodies are Kidney-Specific WNK1 Dependent. Mol Biol Cell. 2017 Dec 13. pii: mbc.E17-08-0529. PMCID: In process.

Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, Brodsky JL, Sobol RW. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. 2017 Feb 10;292(6):2470-2484. PMCID PMC5313114

Buck TM, Jordahl AS, Yates ME, Preston GM, Cook E, Kleyman TR, Brodsky JL. Interactions between intersubunit transmembrane domains regulate the chaperone-dependent degradation of an oligomeric membrane protein. Biochem J. 2017 Feb 1;474(3):357-376. PMCID PMC5423784

Carrisoza-Gaytán R, Wang L, Schreck C, Kleyman TR, Wang WH, Satlin LM. The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD). Am J Physiol Renal Physiol. 2017 Jan 1;312(1):F143-F156. PMCID PMC5283883.

Cerqueira DM, Bodnar AJ, Phua YL, Freer R, Hemker S, Walensky LD, Hukriede NA, Ho J. Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development. FASEBJ 2017, Aug;31(8):3540-3554. PMCID PMC5503708

Cheng F, Birder LA, Kullmann FA, Hornsby J, Watton PN, Watkins S, Thompson M, Robertson AM. Layer-dependent role of collagen recruitment during loading of the rat bladder wall. Biomech Model Mechanobiol. 2017 Oct 16. PMCID: In process.

Dai Y, Chen A, Liu R, Gu L, Sharma S, Cai W, Salem F, Salant DJ, Pippin J, Shankland S, Moeller M, Ghyselinck N, Ding X, Chuang PY, Lee K, He JC. Retinoic acid improves nephrotoxic serum-induced glomerulonephritis through activation of podocyte retinoic acid receptor alpha. Kidney Int. 2017, In press. PMCID In process

Dimitrion P, Zhi Y, Clayton D, Apodaca G, Wilcox, MR, Johnson JW, Nimgaonkar V, D’Aiuto, L. Low-density neuronal cultures from human induced pluripotent stem cells. Mol Neuropsychiatry. 2017, In press. PMCID In process

Emlet DR, Pastor-Soler N, Marciszyn A, Wen X, Gomez H, Humphries WH 4th, Morrisroe S, Volpe JK, Kellum JA. Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells. Am J Physiol Renal Physiol. 2017, Feb 1;312(2):F284-F296. PMCID PMC5336590

Eshbach ML, Kaur A, Rbaibi Y, Tejero J, Weisz OA. Hemoglobin inhibits albumin uptake by proximal tubule cells: implications for sickle cell disease. Am J Physiol Cell Physiol. 2017, Jun 1;312(6):C733-C740. PMCID PMC5494590

Eshbach ML, Weisz OA. Receptor-Mediated Endocytosis in the Proximal Tubule. Annu Rev Physiol. 2017, Feb 10;79:425-448. PMCID PMC5512543

Fan Y, Xiao W, Lee K, Salem F, Wen J, He L, Zhang J, Fei Y, Cheng D, Bao H, Liu Y, Lin F, Jiang G, Guo Z, Wang N, He JC. Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development. J Am Soc Nephrol. 2017, Jul;28(7):2007-2021. PMCID PMC5491287

Fu H, Tian Y, Zhou L, Zhou D, Tan RJ, Stolz DB, Liu Y. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol. 2017, Mar;28(3):785-801. PMCID PMC5328156.

Gallo LI, Dalghi MG, Clayton DR, Ruiz WG, Khandelwal P, Apodaca G. RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells. Am J Physiol Cell Physiol. 2018 Mar 1;314(3):C349-C365. doi: 10.1152/ajpcell.00218.2017. Epub 2017 Nov 22. PubMed PMID: 29167152; PubMed Central PMCID: PMC6335015.

Gleixner AM, Hutchison DF, Sannino S, Bhatia TN, Leak LC, Flaherty PT, Wipf P, Brodsky JL, Leak RK. N-acetyl-L-cysteine protects astrocytes against proteotoxicity without recourse to glutathione. Mol Pharmacol. 2017, In press. PMCID In process

Guerriero CJ, Reutter KR, Augustine AA, Preston GM, Weiberth KF, Mackie TD, Cleveland-Rubeor HC, Bethel NP, Callenberg KM, Nakatsukasa K, Grabe M, Brodsky JL. Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates. Mol Biol Cell. 2017, Jul 15;28(15):2076-2090. PMCID PMC5509421

Hukriede N, Vogt A, de Caestecker M. Drug Discovery to Halt the Progression of AKI to CKD: a case for phenotypic drug discovery in AKI. Nephron. 2017, In press. PMCID In process

Ikeda Y, Zabbarova I, Schaefer CM, Bushnell D, De Groat WC, Kanai A, Bates CM. Fgfr2 is integral for bladder mesenchyme patterning and function. Am J Physiol Renal Physiol. 2017, Apr 1;312(4):F607-F618. PMCID PMC5407073

Jackson EK, Kotermanski SE, Dubey RK, Jackson TC, Kochanek PM. Adenosine production by brain cells. J Neurochem. 2017, Jun;141(5):676-693. PMCID PMC5429183

Jackson EK, Zhang M, Cheng D. Alkaline phosphatase inhibitors attenuate renovascular responses to norepinephrine. Hypertension 2017, 69: 484-493. PMCID PMC5310812

Jackson EK and Mi Z. 8-Aminoguanosine exerts diuretic, natriuretic, and glucosuric activity via conversion to 8-aminoguanine, yet has direct antikaliuretic effects.  J Pharmacol Exp Ther 363: 358–366, 2017. PMCID: PMC5683068.

Jackson EK, ZhanY, Gillespie DD, Zhu X, Cheng D, Jackson TC. SDF-1α induces cardiac fibroblasts, renal microvascular smooth muscle cells and glomerular mesangial cells to proliferate, hypertrophy and produce collagen.  J Am Heart Assoc 6: e007253, 2017.  PMCID: PMC5721794.

Kashlan OB, Kinlough CL, Myerburg MM, Shi S, Chen J, Blobner BM, Buck TM, Brodsky JL, Hughey RP, Kleyman TR. N-linked Glycans are Required on Epithelial Na+ Channel Subunits for Maturation and Surface Expression. Am J Physiol Renal Physiol. 2017 Nov 29:ajprenal.00195.2017. PMCID: In process.

Keller AS, Diederich L, Panknin C, DeLalio LJ, Sherman R, Jackson EK, Kelm M, Cortese-Krott MM and Isakson BE. Possible roles for ATP release from RBCs exclude the cAMP-mediated Panx1 pathway. Am J Physiol Cell Physiol 313: C593-C603, 2017.  PMCID: In process.

Kinsman BJ, Browning KN, Stocker SD. NaCl and osmolarity produce different responses in organum vasculosum of the lamina terminalis neurons, sympathetic nerve activity, and blood pressure. J Physiol 595: 6187-6201, 2017. PMCID: PMC5599491.

Klemens CA, Edinger RS, Kightlinger L, Liu X, Butterworth MB. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017 Jan 6;292(1):375-385. PMCID PMC5217695.

Kleyman TR, Kashlan OB, Hughey RP. Epithelial Na+ channel regulation by extracellular and intracellular Factors. Annu. Rev. Physiol. 2017, In Press. PMCID In process

Kullmann FA, Chang HH, Gauthier C, McDonnell BM, Yeh JC, Clayton DR, Kanai AJ, de Groat WC, Apodaca GL, Birder LA. Acta Physiol. 2017, In press. PMCID In process

Kullmann FA, Clayton DR, Ruiz WG, Wolf-Johnson A, Gauthier C, Kanai AJ, Birder LA, Apodaca G. Urothelial proliferation and regeneration after spinal cord injury. Am J Physiol Renal Physiol. 2017, Jul 1;313(1):F85-F102. PMCID PMC5538841

Kullmann FA. A new player in interstitial cystitis/bladder pain syndrome: platelet-activating factor - PAF and its connection to smoking. Physiol Rep. 2017, Apr;5(7):e13235. PMCID PMC5392521

Lennox AT, Coburn SL, Leech JA, Heidrich EM, Kleyman TR, Wenzel SE, Pilewski JM, Corcoran TE and Myerburg M. ATP12A promotes mucus dysfunction during Type 2 airway inflammation. Sci Rep. Feb 1;8(1):2109. PMCID: In process.

Liu X, Edinger RS, Klemens CA, Phua YL, Bodnar AJ, LaFramboise WA, Ho J, Butterworth MB. A MicroRNA Cluster miR-23-24-27 Is Upregulated by Aldosterone in the Distal Kidney Nephron Where it Alters Sodium Transport. J Cell Physiol. 2017, Jun;232(6):1306-1317. PMCID PMC5426511

Long KR, Shipman KE, Rbaibi Y, Menshikova EV, Ritov VB, Eshback ML, Jiang Y, Jackson EK, Baty CJ and Weisz OA. Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTORdependent pathway.  Mol Biol Cell. 28: 2508-2517, 2017. PMCID: PMC5597323.

Liu, R., Das, B., Xiao, W., Li, Z., Li, H., Lee, K., and He, J.C. A Novel Inhibitor of Homeodomain Interacting Protein Kinase 2 Mitigates Kidney Fibrosis through Inhibition of the TGF-β1/Smad3 Pathway. J Am Soc Nephrol. 2017, Jul;28(7):2133-2143. PMCID PMC5491283

Mackie TD, Kim BY, Subramanya AR, Bain DJ, O'Donnell AF, Welling PA, Brodsky JL. The endosomal trafficking factors CORVET and ESCRT suppress plasma membrane residence of the renal outer medullary potassium channel (ROMK). J Biol Chem. 2018 Jan 8. pii: jbc.M117.819086. PMCID: In process.

Marrone AK, Stolz DB, Bastacky SI, Kostka D, Bodnar AJ, Ho J. MicroRNA-17~92 Is Required for Nephrogenesis and Renal Function. J Am Soc Nephrol. 2017, Jul;25(7):1440-52. PMCID PMC4073423

Montalbetti, N, Rued AC, Taiclet SN, Birder LA, Kullmann FA, Carattino MD. Urothelial tight junction barrier dysfunction sensitizes bladder afferents. eNeuro. 2017, May 24;4(3): ENEURO.0381-16. PMCID PMC5442440

Mukherjee A, Wang Z, Kinlough CL, Poland PA, Marciszyn AL, Montalbetti N, Carattino MD, Butterworth MB, Kleyman TR, Hughey RP. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem. 2017, Mar 10;292(10):4152-4163. PMCID PMC5354509.

Mukherjee E, Maringer K, Papke E, Bushnell D, Schaefer C, Kramann R, Ho J, Humphreys BD, Bates C, Sims-Lucas S. Endothelial marker-expressing stromal cells are critical for kidney formation. Am J Physiol Renal Physiol. 2017 Sep 1;313(3):F611-F620. PMCID: In process.

Muller L, Simms P, Hong CS, Nishimura M, Jackson EK, Watkings S, and Whiteside TL. Human tumor-derived exosomes (TEX) are not internalized by T lymphocytes but signal via surface receptors to induce cellular responses. OncoImmunology 2017, In press. PMCID In process

Nakasone MA, Lewis TA, Walker O, Thakur A, Mansour W, Castañeda CA, Goeckeler-Fried JL, Parlati F, Chou TF, Hayat O, Zhang D, Camara CM, Bonn SM, Nowicka UK, Krueger S, Glickman MH, Brodsky JL, Deshaies RJ, Fushman D. Structural Basis for the Inhibitory Effects of Ubistatins in the Ubiquitin-Proteasome Pathway. Structure. 2017 Dec 5;25(12):1839-1855.e11. PMCID: PMC5731780.

Narla D, Slagle S, Schaefer C, Bushnell D, Bates CM. Loss of peri-Wolffian duct stromal Frs2α expression in mice leads to abnormal ureteric bud induction and vesicoureteral reflux, Pediatric Research. 2017, In press, PMCID In process.

Nickerson KM, Wang Y, Bastacky S, Shlomchik MJ. Toll-like receptor 9 suppresses lupus disease in Fas-sufficient MRL Mice. PLoS One. 2017 Mar 9;12(3):e0173471. PMCID PMC5344451.

O'Donnell BM, Mackie TD, Subramanya AR, Brodsky JL. Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome. J Biol Chem. 2017, Aug 4;292(31):12813-12827. PMCID PMC5546024

Pavlov TS, Levchenko V, Ilatovskaya DV, Li H, Palygin O, Pastor-Soler NM, Hallows KR, Staruschenko A. Lack of Effects of Metformin and AICAR Chronic Infusion on the Development of Hypertension in Dahl Salt-Sensitive Rats. Front Physiol. 2017, Apr 20;8:227. PMCID PMC53975

Pradhan D, Amin M, Hooda S, Dhir R, Bastacky S, Parwani AV. Utility of the laminin immunohistochemical stain in distinguishing invasive from noninvasive urothelial carcinoma. J Cancer Res Ther. 2017 Oct-Dec;13(6):947-950. PMCID: In Process.

Preston GM, Brodsky JL. The evolving role of ubiquitin modification in endoplasmic reticulum-associated degradation. Biochem J. 2017, Feb 15;474(4):445-469. PMCID PMC5425155

Puri P, Schaefer CM, Bushnell D, Taglienti ME, Kreidberg JA, Yoder BK, Bates CM. Ectopic Phosphorylated Creb Marks Dedifferentiated Proximal Tubules in Cystic Kidney Disease. Am J Pathol. 2018 Jan;188(1):84-94. PubMed PMID: 29107072; PMCID: PMC5745541

Ray, E.C. and T.R. Kleyman. An increasingly complex relationship between salt and water. Am J Kidney Dis. 2017, In Press. PMCID In process

Salah EM, Bastacky SI, Jackson EK and Tofovic SP. Captopril attenuates cardiovascular and renal disease in a rat model of heart failure with preserved ejection fraction. J Cardiovasc Pharmacol. In press.  PMCID: In process.

Shi S, Carattino MD. Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes. Bio Protoc. 2017, Apr 20;7(8): e2224. PMCID PMC5493207.

Shi, S., T.M. Buck, C.L. Kinlough, A.L. Marciszyn, R.P. Hughey, M. Chalfie, J.L. Brodsky and T.R. Kleyman. Regulation of the epithelial Na+ channel by paraoxonase-2. J. Biol. Chem. 2017, In press. PMCID In process

Shimizu N, Doyal MF, Goins WF, Kadekawa K, Wada N, Kanai AJ, de Groat WC, Hirayama A, Uemura H, Glorioso JC, Yoshimura N. Morphological changes in different populations of bladder afferent neurons detected by herpes simplex virus (HSV) vectors with cell-type-specific promoters in mice with spinal cord injury. Neuroscience. 2017 Nov 19;364:190-201. PMCID: PMC5768486.

Shun T, Gough AH, Sanker S, Hukriede NA, Vogt A. Exploiting Analysis of Heterogeneity to Increase the Information Content Extracted from Fluorescence Micrographs of Transgenic Zebrafish Embryos. Assay Drug Dev Technol. 2017 Aug/Sep;15(6):257-266. PMCID: PMC5576098

Unruh ML, Pankratz VS, Demko JE, Ray EC, Hughey RP, Kleyman TR. Trial of Amiloride in Type 2 Diabetes with Proteinuria. Kidney Int. Rep. 2017, In press. PMCID: In Process

Ye Z, Needham PG, Estabrooks SK, Whitaker SK, Garcia BL, Misra S, Brodsky JL, Camacho CJ. Symmetry breaking during homodimeric assembly activates an E3 ubiquitin ligase. Sci Rep. 2017, May 11;7(1):1789. PMCID PMC5431976

Zacchi LF, Dittmar JC, Mihalevic MJ, Shewan AM, Schulz BL, Brodsky JL, Bernstein KA. A novel high-throughput yeast genetic screen for factors modifying protein levels of the Early-Onset Torsion Dystonia-associated variant torsinAΔE. Dis Model Mech. 2017, In press. PMCID in process

Zhou D, Fu H, Zhang L, Zhang K, Min Y, Xiao L, Lin L, Bastacky SI, Liu Y. Tubule-Derived Wnts Are Required for Fibroblast Activation and Kidney Fibrosis. J Am Soc Nephrol. 2017, Aug;28(8):2322-2336. PMCID PMC5533232

Zhou D, Tian Y, Sun L, Zhou L, Xiao L, Tan RJ, Tian J, Fu H, Hou FF, Liu Y. Matrix Metalloproteinase-7 Is a Urinary Biomarker and Pathogenic Mediator of Kidney Fibrosis. J Am Soc Nephrol. 2017, Feb;28(2):598-611. PMCID PMC5280025.

Zhu X, Jackson EK. RACK1 regulates angiotensin II-induced contractions of SHR preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol 2017, Apr 1;312(4):F565-F576. PMCID PMC5407068


2018 

Publications 2018:

Amengual J, Guo L, Strong A, Madrigal-Matute J, Wang H, Kaushik S, Brodsky JL, Rader DJ, Cuervo AM, Fisher EA. Autophagy Is Required for Sortilin-Mediated Degradation of Apolipoprotein B100. Circ Res. 2018 Feb 16;122(4):568-582. doi: 10.1161/CIRCRESAHA.117.311240. Epub 2018 Jan 4. PubMed PMID: 29301854; PubMed Central PMCID: PMC5815905.

Balchak DM, Thompson RN, Kashlan OB. The epithelial Na+ channel γ subunit autoinhibitory tract suppresses channel activity by binding the γ subunit's finger-thumb domain interface. J Biol Chem. 2018 Oct 19;293(42):16217-16225. doi: 10.1074/jbc.RA118.004362. PMID: 30131333, PMCID PMC6200929

Bilan, V.P., Schneider, F., Novelli, E.M., Kelly, E.E., Shiva, S., Gladwin, M.T., Jackson, E.K., and Tofovic, S.P. Experimental intravascular hemolysis induces hemodynamic and pathological pulmonary hypertension – Association with accelerated purine metabolism. Pulmonary Circulation, 8(3): doi:10.1177/2045894018791557, 2018. PMC6080084

Birder LA, Kullmann FA. Role of neurogenic inflammation in local communication in the visceral mucosa. Semin Immunopathol. 2018 May;40(3):261-279. doi: 10.1007/s00281-018-0674-0. Epub 2018 Mar 26. Review. PubMed PMID: 29582112; PubMed Central PMCID: PMC5960632.

Boyd-Shiwarski CR, Shiwarski DJ, Roy A, Namboodiri HN, Nkashama LJ, Xie J, McClain KL, Marciszyn A, Kleyman TR, Tan RJ, Stolz DB, Puthenveedu MA, Huang CL, Subramanya AR. Potassium-regulated distal tubule WNK bodies are kidney-specific WNK1 dependent. Mol Biol Cell. 2018 Feb 15;29(4):499-509. doi: 10.1091/mbc.E17-08-0529. Epub 2017 Dec 13. PubMed PMID: 29237822; PubMed Central PMCID: PMC6014176.

D'Aiuto L, Naciri J, Radio N, Tekur S, Clayton D, Apodaca G, Di Maio R, Zhi Y, Dimitrion P, Piazza P, Demers M, Wood J, Chu C, Callio J, McClain L, Yolken R, McNulty J, Kinchington P, Bloom D, Nimgaonkar V. Generation of three-dimensional human neuronal cultures: application to modeling CNS viral infections. Stem Cell Res Ther. 2018 May 11;9(1):134. doi: 10.1186/s13287-018-0881-6. PubMed PMID: 29751846; PubMed Central PMCID: PMC5948884.

Desai PB, San Agustin JT, Stuck MW, Jonassen JA, Bates CM, Pazour GJ. Ift25 is not a cystic kidney disease gene but is required for early steps of kidney development. Mech Dev. 2018 Jun;151:10-17. doi: 10.1016/j.mod.2018.04.001. Epub 2018 Apr 4. PubMed PMID: 29626631; PMCID: PMC5972074.

Doonan LM, Fisher EA, Brodsky JL. Can modulators of apolipoproteinB biogenesis serve as an alternate target for cholesterol-lowering drugs? Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jul;1863(7):762-771. doi: 10.1016/j.bbalip.2018.03.010. PMID:29627384, PMCID PMC5953829

Espiritu EB, Crunk AE, Bais A, Hochbaum D, Cervino AS, Phua YL, Butterworth MB, Goto T, Ho J, Hukriede NA, Cirio MC. The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development. Sci Rep. 2018 Oct 30;8(1):16029. doi: 10.1038/s41598-018-34038-x. PubMed PMID: 30375416; PubMed Central PMCID: PMC6207768.

Gallo LI, Dalghi MG, Clayton DR, Ruiz WG, Khandelwal P, Apodaca G. RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells. Am J Physiol Cell Physiol. 2018 Mar 1;314(3):C349-C365. doi: 10.1152/ajpcell.00218.2017. Epub 2017 Nov 22. PubMed PMID: 29167152; PubMed Central PMCID: PMC6335015.

Hager NA, Krasowski CJ, Mackie TD, Kolb AR, Needham PG, Augustine AA, Dempsey A, Szent-Gyorgyi C, Bruchez MP, Bain DJ, Kwiatkowski AV, O'Donnell AF, Brodsky JL. Select α-arrestins control cell-surface abundance of the mammalian Kir2.1 potassium channel in a yeast model. J Biol Chem. 2018 Jul 13;293(28):11006-11021. doi: 10.1074/jbc.RA117.001293. Epub 2018 May 21. PubMed PMID: 29784874; PubMed Central PMCID: PMC6052216.

Ikeda Y, Wolf-Johnston A, Roppolo JR, Buffington CAT, Birder L. Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin. Int Neurourol J. 2018 Dec;22(4):246-251. doi: 10.5213/inj.1836276.138. PMID: 30599495, PMCID PMC6312968

Jackson EK, Mi Z, Kleyman TR, Cheng D. 8-Aminoguanine Induces Diuresis, Natriuresis, and Glucosuria by Inhibiting Purine Nucleoside Phosphorylase and Reduces Potassium Excretion by Inhibiting Rac1. J Am Heart Assoc. 2018 Nov 6;7(21):e010085. doi: 10.1161/JAHA.118.010085. PMID: 30608204, PMCID PMC6404173

Jackson, E.K., Mi, E., Ritov, V.B., and Gillespie, D.G. Extracellular ubiquitin(1-76) and ubiquitin(1-74) regulate cardiac fibroblast proliferation. Hypertension 72:909-917, 2018. PMCID PMC6206878

Jackson, E.K., Gillespie, D.G., Mi, Z., and Cheng, D. Adenosine receptors influence hypertension in Dahl salt-sensitive rats: dependence on receptor subtype, salt diet, and sex. Hypertension, 72:511-521, 2018. PMCID PMC6053282

Jackson, T.C., Dixon, C.E., Janesko-Feldman, K., Vagni, V., Kotermanski, S.E., Jackson, E.K., and Kochanek, P.M.: Acute physiology and neurologic outcomes after brain injury in SCOP/PHLPP1 KO mice. Scientific Reports 8: 7158, 2018. PMCID PMC5940799

Jobbagy S, Tan RJ. Nitrolipids in kidney physiology and disease. Nitric Oxide. 2018 Mar 29. pii: S1089-8603(18)30006-5. doi: 10.1016/j.niox.2018.03.021 [Epub ahead of print]. PMID 29605557, PMCID PMC6163094.

Joshi S, Wang T, Araujo TLS, Sharma S, Brodsky JL, Chiosis G. Adapting to stress - chaperome networks in cancer. Nat Rev Cancer. 2018 Sep;18(9):562-575. doi: 10.1038/s41568-018-0020-9. PMID:29795326 PMCID PMC6108944

Kashlan OB, Kinlough CL, Myerburg MM, Shi S, Chen J, Blobner BM, Buck TM, Brodsky JL, Hughey RP, Kleyman TR. N-linked glycans are required on epithelial Na+ channel subunits for maturation and surface expression. Am J Physiol Renal Physiol. 2018 Mar 1;314(3):F483-F492. doi: 10.1152/ajprenal.00195.2017. Epub 2017 Nov 29. PMID: 29187368; PMCID: PMC5899221.

Kharade SV, Kurata H, Bender AM, Blobaum AL, Figueroa EE, Duran A, Kramer M, Days E, Vinson P, Flores D, Satlin LM, Meiler J, Weaver CD, Lindsley CW, Hopkins CR, Denton JS. Discovery, Characterization, and Effects on Renal Fluid and Electrolyte Excretion of the Kir4.1 Potassium Channel Pore Blocker, VU0134992. Mol Pharmacol. 2018 Aug;94(2):926-937. doi: 10.1124/mol.118.112359. PMID: 29895592, PMCID PMC6041953

Kleyman, T.R., Kashlan, O.B., and R.P. Hughey, R.P.: Epithelial Na+ channel regulation by extracellular and intracellular Factors. Annual. Review of Physiology. 80: 263–281, 2018. PMCID PMC5811403

Kullmann FA, Beckel JM, McDonnell B, Gauthier C, Lynn AM, Wolf-Johnston A, Kanai A, Zabbarova IV, Ikeda Y, de Groat WC, Birder LA. Involvement of TRPM4 in detrusor overactivity following spinal cord transection in mice. Naunyn Schmiedebergs Arch Pharmacol. 2018 Nov;391(11):1191-1202. doi: 10.1007/s00210-018-1542-0. PMID: 30054681, PMCID PMC6186176

Kullmann FA, Chang HH, Gauthier C, McDonnell BM, Yeh JC, Clayton DR, Kanai AJ, de Groat WC, Apodaca GL, Birder LA. Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing. Acta Physiol (Oxf). 2018 Feb;222(2). doi: 10.1111/apha.12919. Epub 2017 Aug 8. PubMed PMID: 28719042; PubMed Central PMCID: PMC5963688.

Kullmann FA, McDonnell BM, Wolf-Johnston AS, Lynn AM, Giglio D, Getchell SE, Ruiz WG, Zabbarova IV, Ikeda Y, Kanai AJ, Roppolo JR, Bastacky SI, Apodaca G, Buffington CAT, Birder LA. Inflammation and Tissue Remodeling in the Bladder and Urethra in Feline Interstitial Cystitis. Front Syst Neurosci. 2018 Apr 13;12:13. doi: 10.3389/fnsys.2018.00013. eCollection 2018. Erratum in: Front Syst Neurosci. 2018 Nov 08;12:58. PubMed PMID: 29706873; PubMed Central PMCID: PMC5908978.

Mackie TD, Brodsky JL. Investigating Potassium Channels in Budding Yeast: A Genetic Sandbox. Genetics. 2018 Jul;209(3):637-650. doi: 10.1534/genetics.118.301026. PMID:29967058. PMCID PMC6028241

Montalbetti N, Rooney JG, Marciszyn AL, Carattino MD. ASIC3 fine-tunes bladder sensory signaling. Am J Physiol Renal Physiol. 2018 Oct 1;315(4):F870-F879. doi: 10.1152/ajprenal.00630.2017. Epub 2018 Mar 21. PubMed PMID: 29561183; PMCID: PMC6230751.

Pabon NA, Xia Y, Estabrooks SK, Ye Z, Herbrand AK, Süß E, Biondi RM, Assimon VA, Gestwicki JE, Brodsky JL, Camacho CJ, Bar-Joseph Z. Predicting protein targets for drug-like compounds using transcriptomics. PLoS Comput Biol. 2018 Dec 7;14(12):e1006651. doi: 10.1371/journal.pcbi.1006651. eCollection 2018 Dec. PMID: 30532261, PMCID PMC6300300

Preston GM, Guerriero CJ, Metzger MB, Michaelis S, Brodsky JL. Substrate Insolubility Dictates Hsp104-Dependent Endoplasmic-Reticulum-Associated Degradation. Mol Cell. 2018 Apr 19;70(2):242-253.e6. doi: 10.1016/j.molcel.2018.03.016. PMID:29677492, PMCID5912696

Ramani K, Tan RJ, Zhou D, Coleman BM, Jawale CV, Liu Y, Biswas PS. IL-17 Receptor Signaling Negatively Regulates the Development of Tubulointerstitial Fibrosis in the Kidney. Mediators Inflamm. 2018 Oct 14;2018:5103672. doi: 10.1155/2018/5103672. eCollection 2018. PubMed PMID: 30405320; PubMed Central PMCID: PMC6204168.

Ray EC, Miller RG, Demko JE, Costacou T, Kinlough CL, Demko CL, Unruh ML, Orchard TJ, Kleyman TR. Urinary Plasmin(ogen) as a Prognostic Factor for Hypertension. Kidney Int Rep. 2018 Nov;3(6):1434-1442. doi: 10.1016/j.ekir.2018.06.007. eCollection 2018 Nov. PubMed PMID: 30450470; PubMed Central PMCID: PMC6224670.

Ray EC. ENaC blockade in proteinuria-associated extracellular fluid volume overload - effective but risky. Physiol Rep. 2018 Sep;6(17):e13835. doi: 10.14814/phy2.13835. PMID: 30178569, PMCID PMC6121123

Rondon-Berrios H, Tandukar S, Mor MK, Ray EC, Bender FH, Kleyman TR, Weisbord SD. Urea for the Treatment of Hyponatremia. Clin J Am Soc Nephrol. 2018 Nov 7;13(11):1627-1632. doi: 10.2215/CJN.04020318. PMID: 30181129, PMCID PMC6237061

Sanders AP, Saland JM, Wright RO, Satlin L. Perinatal and childhood exposure to environmental chemicals and blood pressure in children: a review of literature 2007-2017. Pediatr Res. 2018 Aug;84(2):165-180. doi: 10.1038/s41390-018-0055-3. Epub 2018 Jun 9. Review. PubMed PMID: 29884847; PubMed Central PMCID: PMC6185812.

Sanders AP, Svensson K, Gennings C, Burris HH, Oken E, Amarasiriwardena C, Basnet P, Pizano-Zarate ML, Schnaas L, Tamayo-Ortiz M, Baccarelli AA, Satlin LM, Wright RO, Tellez-Rojo MM. Prenatal lead exposure modifies the effect of shorter gestation on increased blood pressure in children. Environ Int. 2018 Nov;120:464-471. doi: 10.1016/j.envint.2018.08.038. PMID: 30145310, PMCID PMC6354251.

Sannino S, Guerriero CJ, Sabnis AJ, Stolz DB, Wallace CT, Wipf P, Watkins SC, Bivona TG, Brodsky JL. Compensatory increases of select proteostasis networks after Hsp70 inhibition in cancer cells. J Cell Sci. 2018 Sep 5;131(17). pii: jcs217760. doi: 10.1242/jcs.217760. PubMed PMID: 30131440; PMCID: PMC6140321.

Shamir AR, Karembelkar A, Yabes J, Yao Y, Miskulin D, Gassman J, Ploth D, Negrea L, Paine S, Rahman M, Kwong RY, Zager P, Jhamb M. Association of Intradialytic Hypertension with Left Ventricular Mass in Hypertensive Hemodialysis Patients Enrolled in the Blood Pressure in Dialysis (BID) Study. Kidney Blood Press Res. 2018;43(3):882-892. doi: 10.1159/000490336. Epub 2018 May 30. PubMed PMID: 29870977; PubMed Central PMCID: PMC6487648.

Sheng S, Chen J, Mukherjee A, Yates ME, Buck TM, Brodsky JL, Tolino MA, Hughey RP, Kleyman TR. Thumb domains of the three epithelial Na+ channel subunits have distinct functions. J Biol Chem. 2018 Nov 9;293(45):17582-17592. doi: 10.1074/jbc.RA118.003618. PMID: 30228189. PMCID PMC6231141

Sun Z, Brodsky JL. The degradation pathway of a model misfolded protein is determined by aggregation propensity. Mol Biol Cell. 2018 Jun 15;29(12):1422-1434. doi: 10.1091/mbc.E18-02-0117. Epub 2018 Apr 24. PubMed PMID: 29688814; PMCID: PMC6014095.

Theodoraki, M.N., Hoffmann, T.K., Jackson, E.K., and Whiteside, T.L.: Exosomes in HNSCC plasma as surrogate markers of tumour progression and immune competence. Clinical and Experimental Immunology 194:67-78, 2018. PMCID PMC6156813

Truschel ST, Clayton DR, Beckel JM, Yabes JG, Yao Y, Wolf-Johnston A, Birder LA, Apodaca G. Age-related endolysosome dysfunction in the rat urothelium. PLoS One. 2018 Jun 8;13(6):e0198817. doi: 10.1371/journal.pone.0198817. eCollection 2018. PubMed PMID: 29883476; PMCID: PMC5993304.

Wen X, Cui L, Morrisroe S1, Maberry D Jr, Emlet D, Watkins S, Hukriede NA, Kellum JA. A zebrafish model of infection-associated acute kidney injury. Am J Physiol Renal Physiol. 2018 Aug 1;315(2):F291-F299. doi: 10.1152/ajprenal.00328.2017. PMCID PMC6139521

Wozniak KL, Phelps WA, Tembo M, Lee MT, Carlson AE. The TMEM16A channel mediates the fast polyspermy block in Xenopus laevis. J Gen Physiol. 2018 Sep 3;150(9):1249-1259. doi: 10.1085/jgp.201812071. PMID: 30012842, PMCID PMC6122928

Wozniak KL, Tembo M, Phelps WA, Lee MT, Carlson AE. PLC and IP3-evoked Ca2+ release initiate the fast block to polyspermy in Xenopus laevis eggs. J Gen Physiol. 2018 Sep 3;150(9):1239-1248. doi: 10.1085/jgp.201812069. PMID: 30012841, PMCID PMC6122927

Ziebart, A., Huber, U., Jaekle, S., Laban, S., Doescher, J., Hoffmann, T.K., Brunner, C., Jackson, E.K., and Schuler, P.J.: The influence of chemotherapy on adenosine-producing B cells in patients with head and neck cancer. Oncotarget 9: 5834-5847, 2018. PMCID PMC5814178






2019 

Publications 2019:

Brilli Skvarca L, Han HI, Espiritu EB, Missinato MA, Rochon ER, McDaniels MD, Bais AS, Roman BL, Waxman JS, Watkins SC, Davidson AJ, Tsang M, Hukriede NA. Enhancing regeneration after acute kidney injury by promoting cellular dedifferentiation in zebrafish. Dis Model Mech. 2019 Apr 5;12(4). pii: dmm037390. doi: 10.1242/dmm.037390.PMID: 30890583, PMCID PMC6505474

Chiang AN, Liang M, Dominguez-Meijide A, Masaracchia C, Goeckeler-Fried JL, Mazzone CS, Newhouse DW, Kendsersky NM, Yates ME, Manos-Turvey A, Needham PG, Outeiro TF, Wipf P, Brodsky JL. Synthesis and evaluation of esterified Hsp70 agonists in cellular models of protein aggregation and folding. Bioorg Med Chem. 2019 Jan 1;27(1):79-91. doi: 10.1016/j.bmc.2018.11.011. Epub 2018 Nov 15. PubMed PMID: 30528127; PubMed Central PMCID: PMC6298824.

Chiba T, Peasley KD, Cargill KR, Maringer KV, Bharathi SS, Mukherjee E, Zhang Y, Holtz A, Basisty N, Yagobian SD, Schilling B, Goetzman ES, Sims-Lucas S. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI. J Am Soc Nephrol. 2019 Dec;30(12):2384-2398. PMCID: PMC6900790

Dalghi MG, Clayton DR, Ruiz WG, Al-Bataineh MM, Satlin LM, Kleyman TR, Ricke WA, Carattino MD, Apodaca G. Expression and distribution of PIEZO1 in the mouse urinary tract. Am J Physiol Renal Physiol. 2019 Aug 1;317(2):F303-F321. doi: 10.1152/ajprenal.00214.2019. Epub 2019 Jun 5. PMID: 31166705.

Dalghi MG, Montalbetti N, Carattino MD, Apodaca G. The Urothelium: Life in a Liquid Environment. Physiol Rev. 2020 Mar 19. doi: 10.1152/physrev.00041.2019. [Epub ahead of print] PMID: 32191559; PMID: 32191559; “PMC Journal - In Process”

Doonan LM, Guerriero CJ, Preston GM, Buck TM, Khazanov N, Fisher EA, Senderowitz H, Brodsky JL. Hsp104 Facilitates the Endoplasmic Reticulum Associated Degradation of Disease-Associated and Aggregation-Prone Substrates. Protein Sci. 2019 May 3. doi: 10.1002/pro.3636. PMID: 31050848, PMCID in process

Eaton AF, Clayton DR, Ruiz WG, Griffiths SE, Rubio ME, Apodaca G. Expansion and contraction of the umbrella cell apical junctional ring in response to bladder filling and voiding. Mol Biol Cell. 2019 Jul 22;30(16):2037-2052. doi: 10.1091/mbc. E19-02-0115. PMID: 31166831; PMCID: PMC6727774

Espiritu EB, Jiang H, Moreau-Marquis S, Sullivan M, Yan K, Beer Stolz D, Sampson MG, Hukriede NA, Swiatecka-Urban A. The human nephrin Y1139RSL motif is essential for podocyte foot process organization and slit diaphragm formation during glomerular development. J Biol Chem. 2019 Jul 12;294(28):10773-10788. doi: 10.1074/jbc.RA119.008235. PMID: 31152064; PMCID: PMC6635437 [Available on 2020-07-12]

Gliozzi ML, Rbaibi Y, Long KR, Vitturi DA, Weisz OA. Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease. Am J Physiol Cell Physiol. 2019 Nov 1;317(5):C993-C1000. doi: 10.1152/ajpcell.00287.2019. PMID: 31509446; PMCID: PMC6879883 [Available on 2020-11-01]

Guerriero CJ, Gomez YK, Daskivich GJ, Reutter KR, Augustine AA, Weiberth KF, Nakatsukasa K, Grabe M, Brodsky JL. Harmonizing experimental data with modeling to predict membrane protein insertion in yeast. Biophys. J., 2019 117: 668-678. PMID: 31399214; PMCID: PMC6712491

Han HI, Skvarca LB, Espiritu EB, Davidson AJ, Hukriede NA. The role of macrophages during acute kidney injury: destruction and repair. Pediatr Nephrol. 2019 Apr;34(4):561-569. doi: 10.1007/s00467-017-3883-1. PMID: 29383444, PMCID PMC6066473

Jackson, T.C., Kotermansi, S.E., Kochanek, P.M. and Jackson, E.K.: Oxidative stress induces release of 2’-AMP from microglia. Brain Research 1706: 101-109, 2019. PMCID PMC6363863

Jackson, E.K., Mi, Z., Keri Janesko-Feldman, K., Jackson, T.C., and Kochanek, M.K. 2′,3′-cGMP exists in vivo and comprises a 2′,3′-cGMP-guanosine pathway. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 316: R783–R790, 2019. PMC6620655

Kamei CN, Gallegos TF, Liu Y, Hukriede N, Drummond IA. Wnt signaling mediates new nephron formation during zebrafish kidney regeneration. Development. 2019 Apr 29;146(8). pii: dev168294. doi: 10.1242/dev.168294. PMID: 31036548, PMCID In process

Levin-Schwartz Y, Curtin P, Svensson K, Fernandez NF, Kim-Schulze S, Hair GM, Flores D, Pantic I, Tamayo-Ortiz M, Luisa Pizano-Zárate M, Gennings C, Satlin LM, Baccarelli AA, Tellez-Rojo MM, Wright RO, Sanders AP. Length of gestation and birth weight are associated with indices of combined kidney biomarkers in early childhood. PLoS One. 2019 Dec 31;14(12):e0227219. doi: 10.1371/journal.pone.0227219. PMID: 31891650; PMCID: PMC6938375

Macatangay, B.J.C., Jackson, E.K., Abebe, K., Comer, D., Cyktor, J., Klamar-Blain, C., Borowski, L., Gillespie, D., Klingman, K., Mellors, J.W., Charles R Rinaldo, C.R., and Riddler, S.A.: A randomized, placebo-controlled, pilot clinical trial of dipyridamole to decrease HIV-associated chronic inflammation. The Journal of Infectious Diseases, jiz344, https://doi.org/10.1093/infdis/jiz344, 2019. Open Access.

Mann JR, Gleixner AM, Mauna JC, Gomes E, DeChellis-Marks MR, Needham PG, Copley KE, Hurtle B, Portz B, Pyles NJ, Guo L, Calder CB, Wills ZP, Pandey UB, Kofler JK, Brodsky JL, Thathiah A, Shorter J, Donnelly CJ. RNA Binding Antagonizes Neurotoxic Phase Transitions of 19. TDP-43. Neuron. 2019 Apr 17;102(2):321-338.e8. doi: 10.1016/j.neuron.2019.01.048. PMID: 30826182, PMCID PMC6472983

Mitash N, Mu F, Donovan JE, Myerburg MM, Ranganathan S, Greene CM, Swiatecka-Urban A. Transforming Growth Factor-β1 Selectively Recruits microRNAs to the RNA-Induced Silencing Complex and Degrades CFTR mRNA under Permissive Conditions in Human Bronchial Epithelial Cells. Int J Mol Sci. 2019 Oct 5;20(19). pii: E4933. doi: 10.3390/ijms20194933. PMID: 31590401; PMCID: PMC6801718

Montalbetti N, Rooney JG, Rued AC, Carattino MD. Molecular determinants of afferent sensitization in a rat model of cystitis with urothelial barrier dysfunction. J Neurophysiol. 2019 Sep 1;122(3):1136-1146. doi: 10.1152/jn.00306.2019. PMID: 31314637; PMCID: PMC6766738 [Available on 2020-09-01]

Montalbetti N, Stocker SD, Apodaca G, Bastacky SI, Carattino MD. Urinary K(+) promotes irritative voiding symptoms and pain in the face of urothelial barrier dysfunction. Sci Rep. 2019 Apr 2;9(1):5509. doi: 10.1038/s41598-019-41971-y. PubMed PMID: 30940909; PMCID: PMC6445132.

Mutchler, S.M. and T.R. Kleyman. New insights regarding epithelial Na+ channel regulation and its role in the kidney, immune system and vasculature. Current Opinion in Nephrololgy and Hypertension 28:113-119, 2019. PMCID PMC6349474

Nau GJ, Horzempa J, O'Dee D, Brown MJ, Russo BC, Hernandez A, Dillon ST, Cheng J, Kane LP, Sanker S, Hukriede NA. A predicted Francisella tularensis DXD-motif glycosyltransferase blocks immune activation. Virulence. 2019 Dec;10(1):643-656. doi: 10.1080/21505594.2019.1631662. PMID: 31314675; PMCID: PMC6650193

Needham PG, Guerriero CJ, Brodsky JL. Chaperoning Endoplasmic Reticulum Associated Degradation (ERAD) and Protein Conformational Diseases. Cold Spring Harbor Perspectives in Biology 2019 11(8). PMID: 30670468; PMCID: PMC6671943

Ong J, Kinsman BJ, Sved AF, Rush BM, Tan RJ, Carattino MD, Stocker SD. Renal sensory nerves increase sympathetic nerve activity and blood pressure in 2-kidney 1-clip hypertensive mice. J Neurophysiol. 2019 Jul 1;122(1):358-367. doi: 10.1152/jn.00173.2019. Epub 2019 May 15. PMID: 31091159

Phua YL, Chen KH, Hemker SL, Marrone AK, Bodnar AJ, Liu X, Clugston A, Kostka D, Butterworth MB, Ho J. Loss of miR-17~92 results in dysregulation of Cftr in nephron progenitors. Am J Physiol Renal Physiol. 2019 May 1;316(5):F993-F1005. doi: 10.1152/ajprenal.00450.2018. PMID: 30838872, PMCID In process.

Ren Q, Gliozzi ML, Rittenhouse NL, Edmunds LR, Rbaibi Y, Locker JD, Poholek AC, Jurczak MJ, Baty CJ, Weisz OA. Shear stress and oxygen availability drive differential changes in opossum kidney proximal tubule cell metabolism and endocytosis. Traffic. 2019 Jun;20(6):448-459. doi: 10.1111/tra.12648. PMID: 30989771; PMCID: PMC7055529

Repetti RL, Meth J, Sonubi O, Flores D, Satlin LM, Rohatgi R. Cellular cholesterol modifies flow-mediated gene expression. Am J Physiol Renal Physiol. 2019 Oct 1;317(4):F815-F824. doi: 10.1152/ajprenal.00196.2019. PMID: 31364378; PMCID: PMC6843042 [Available on 2020-10-01]

Salah E, Bastacky SI, Jackson EK, Tofovic SP. 2-Methoxyestradiol Attenuates Angiotensin II-Induced Hypertension, Cardiovascular Remodeling, and Renal Injury. J Cardiovasc Pharmacol. 2019 Mar;73(3):165-177. doi: 10.1097/FJC.0000000000000649. PubMed PMID: 30839510; PubMed Central PMCID: PMC6404767.

Sharma A, Cherukuri A, Mehta RB, Sood P, Hariharan S. High Calcineurin Inhibitor Intrapatient Variability Is Associated With Renal Allograft Inflammation, Chronicity, and Graft Loss. Transplant Direct. 2019 Feb;5(2):e424. doi: 10.1097/TXD.0000000000000862. eCollection 2019 Feb. PubMed PMID: 30882028; PubMed Central PMCID: PMC6415973.

Sun Z, Brodsky JL. Protein quality control in the secretory pathway. J. Cell Biol. 2019 218: 3171-3187. PMID: 31537714; PMCID: PMC6781448

Tan RJ, Li Y, Rush BM, Cerqueira DM, Zhou D, Fu H, Ho J, Beer Stolz D, Liu Y. Tubular injury triggers podocyte dysfunction by β-catenin-driven release of MMP-7. JCI Insight. 2019 Dec 19;4(24):e122399. doi: 10.1172/jci.insight.122399. PMID: 31743113. Open Access.

Tembo M, Wozniak KL, Bainbridge RE, Carlson AE. Phosphatidylinositol 4,5-bisphosphate (PIP2) and Ca2+ are both required to open the Cl- channel TMEM16A. J Biol Chem. 2019 Aug 16;294(33):12556-12564. doi: 10.1074/jbc.RA118.007128. PMID: 31266809; PMCID: PMC6699839 [Available on 2020-08-16]

Tomich AD, Klontz EH, Deredge D, Barnard JP, McElheny CL, Eshbach ML, Weisz OA, Wintrode P, Doi Y, Sundberg EJ, Sluis-Cremer N. Small-Molecule Inhibitor of FosA Expands Fosfomycin Activity to Multidrug-Resistant Gram-Negative Pathogens. Antimicrob Agents Chemother. 2019 Feb 26;63(3). pii: e01524-18. doi: 10.1128/AAC.01524-18. PMID: 30642934, PMCID PMC6395921

Wang XP, Im SJ, Balchak DM, Montalbetti N, Carattino MD, Ray EC, Kashlan OB. Murine epithelial sodium (Na+) channel regulation by biliary factors. J Biol Chem. 2019 May 15. pii: jbc.RA119.007394. doi: 10.1074/jbc.RA119.007394. PMID: 31092599. PMCID In process

Wang X, Chen J, Shi S, Sheng S, Kleyman TR. Analyses of epithelial Na+ channel variants reveal that an extracellular β-ball domain critically regulates ENaC gating. J Biol Chem. 2019 Nov 8;294(45):16765-16775. doi: 10.1074/jbc.RA119.010001. PMID: 31551351; PMCID: PMC6851303 [Available on 2020-11-08]

Xiao L, Xu B, Zhou L, Tan RJ, Zhou D, Fu H, Li A, Hou FF, Liu Y. Wnt/β-catenin regulates blood pressure and kidney injury in rats. Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1313-1322. doi: 10.1016/j.bbadis.2019.01.027. Epub 2019 Jan 30. PubMed PMID: 30710617; PMCID: PMC6502648.

Zhou D, Fu H, Liu S, Zhang L, Xiao L, Bastacky SI, Liu Y. Early activation of fibroblasts is required for kidney repair and regeneration after injury. FASEB J. 2019 Nov;33(11):12576-12587. doi: 10.1096/fj.201900651RR. Epub 2019 Aug 28. PMID: 31461626.

Zhou X, Kinlough CL, Hughey RP, Jin M, Inoue H, Etling E, Modena BD, Kaminski N, Bleecker ER, Meyers DA, Jarjour NN, Trudeau JB, Holguin F, Ray A, Wenzel SE. Sialylation of MUC4β N-glycans by ST6GAL1 orchestrates human airway epithelial cell differentiation associated with type-2 inflammation. JCI Insight. 2019 Mar 7;4(5). pii: 122475. doi: 10.1172/jci.insight.122475. eCollection 2019 Mar 7.PMID: 30730306, PMCID PMC6483602

2020 

Publications 2020:

Carattino MD, Montalbetti N. Acid-sensing ion channels in sensory signaling. Am J Physiol Renal Physiol. 2020 Mar 1;318(3):F531-F543. doi: 10.1152/ajprenal.00546.2019. Epub 2020 Jan 27. PMID: 31984789 PMCID: PMC7099506 [Available on 2021-03-01]

Carrisoza-Gaytan R, Ray EC, Flores D, Marciszyn AL, Wu P, Liu L, Subramanya AR, Wang W, Sheng S, Nkashama LJ, Chen J, Jackson EK, Mutchler SM, Heja S, Kohan DE, Satlin LM, Kleyman TR. Intercalated cell BKα subunit is required for flow- induced K+ secretion. JCI Insight. 2020 Apr 7:130553. doi: 10.1172/jci.insight.130553. Epub ahead of print. PMID: 32255763.

Chen Q, Yu J, Rush BM, Stocker SD,  Tan, RJ, and Kim K.  Noninvasive assessment of renal microvasculature changes in mouse acute kidney injury using ultrasound super-resolution imaging.  Kidney Int., 2020, in press. doi.org/10.1016/j.kint.2020.02.011

Cruz DF, Mitash N, Farinha CM, Swiatecka-Urban A. TGF-β1 Augments the Apical Membrane Abundance of Lemur Tyrosine Kinase 2 to Inhibit CFTR-Mediated Chloride Transport in Human Bronchial Epithelia. Front Cell Dev Biol. 2020 Feb 7;8:58. doi: 10.3389/fcell.2020.00058. eCollection 2020. PMID: 32117984; PMCID: PMC7018669

Dalghi MG, Montalbetti N, Carattino MD, Apodaca G. The Urothelium: Life in a Liquid Environment. Physiol Rev. 2020 Mar 19. doi: 10.1152/physrev.00041.2019. [Epub ahead of print] PMID: 32191559; PMID: 32191559; “PMC Journal - In Process”

Dubey, R.K., Baruscotti, I., Stiller, R., Fingerle, J., Gillespie, D.G., Mi, Z., Imthurn, B., Rosselli, M., and Jackson, E.K.: Adenosine prevents progenitor smooth muscle cell (P-SMC) infiltration in injury-induced neointimal formation and inhibits human P-SMC growth via A2B receptors. Hypertension 75: 109-118, 2020. PMC7009319

Espiritu EB, Jiang H, Moreau-Marquis S, Sullivan M, Yan K, Beer Stolz D, Sampson MG, Hukriede NA, Swiatecka-Urban A. The human nephrin Y1139RSL motif is essential for podocyte foot process organization and slit diaphragm formation during glomerular development. J Biol Chem. 2019 Jul 12;294(28):10773-10788. doi: 10.1074/jbc.RA119.008235. PMID: 31152064; PMCID: PMC6635437 [Available on 2020-07-12]

Estabrooks S, Brodsky JL. Regulation of CFTR Biogenesis by the Proteostatic Network and Pharmacological Modulators. Int J Mol Sci. 2020 Jan 10;21(2), PMID: 31936842; PMCID: PMC7013518

Ferro-Novick S, Reggiori F, Brodsky JL. ER-Phagy, ER Homeostasis, and ER Quality Control: Implications for Disease. Trends in biochemical sciences. 2021 January 25. PubMed PMID: 33509650; DOI: 10.1016/j.tibs.2020.12.013.
 
Frindt G, Shi S, Kleyman TR, Palmer LG. Cleavage state of γENaC in mouse and rat kidneys. Am J Physiol Renal Physiol. 2021 Mar 1;320(3):F485-F491. Epub 2021 Feb 1. PMID: 33522411. PMCID: PMC7988816 (available on 2022-03-01)

Galley, J.C., Hahn, S.A., Miller, M.P., Durgin, B.G., Jackson, E.K., Stocker, S.D., and Straub, A.C.:  Angiotensin II augments renal vascular smooth muscle sGC expression via an AT1R-FoxO transcription factor signaling axis.  British Journal of Pharmacology, 2021. May 7;. doi: 10.1111/bph.15522. [Epub ahead of print] PubMed PMID: 33963547

Gliozzi ML, Espiritu EB, Shipman KE, Rbaibi Y, Long KR, Roy N, Duncan AW, Lazzara MJ, Hukriede NA, Baty CJ, Weisz OA. Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model. J Am Soc Nephrol. 2020 Jan;31(1):67-83. doi: 10.1681/ASN.2019020125. PMID: 31676724; PMCID: PMC6934997

Gliozzi ML, Rbaibi Y, Long KR, Vitturi DA, Weisz OA. Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease. Am J Physiol Cell Physiol. 2019 Nov 1;317(5):C993-C1000. doi: 10.1152/ajpcell.00287.2019. PMID: 31509446; PMCID: PMC6879883 [Available on 2020-11-01]

Gordon RA, Tilstra JS, Marinov A, Nickerson KM, Bastacky SI, Shlomchik MJ. Murine lupus is neutrophil elastase-independent in the MRL.Faslpr model. PLoS One. 2020 Apr 3;15(4):e0226396. doi: 10.1371/journal.pone.0226396. PMID: 32243431.

Hemker SL, Cerqueira DM, Bodnar AJ, Cargill KR, Clugston A, Anslow MJ, Sims-Lucas S, Kostka D, Ho J. Deletion of hypoxia-responsive microRNA-210 results in a sex-specific decrease in nephron number. FASEB J. 2020 April;34(4):5782-5799. PubMed PMID: 32141129; PubMed Central PMCID: PMC7136145; DOI: 10.1096/fj.201902767R.

Jackson, E.K., and Gillespie, D.G.: Combinatorial Effects of DPP4 inhibition, NPY1-36, PYY1-36, SDF-1α and a hypertensive genetic background on cell proliferation and collagen production. Journal of Pharmacology and Experimental Therapeutics, 373: 135-148, 2020. PMID: 32015161, Open Access.

Jackson, E.K., Gillespie, D.G, Cheng, D., Mi, Z., and V. Menshikova, E.V.: Validation of the etheno-bridge method to assess extracellular metabolism of adenine nucleotides. Purinergic Signaling, 2020 (In Press). PMC Journal – In Process

Jackson, E.K., Cheng, D., Ritov, V.B., and Mi, Z.:  Alkaline phosphatase activity is a key determinant of vascular responsiveness to norepinephrine.  Hypertension 76: 1308-1318, 2020. PMC7484402, PMID: 32829665

Jackson, E.K., Gillespie, D.G, Cheng, D., Mi, Z., and V. Menshikova, E.V.:  Characterization of the N6-etheno-bridge method to assess extracellular metabolism of adenine nucleotides: detection of a possible role for purine nucleoside phosphorylase in adenosine metabolism.  Purinergic Signaling 16: 187-211, 2020. PMC7367995, PMID: 32367441
 
Jackson, E.K., Mi, Z, Gillespie, D.G., and Tofovic, S.P.:  Long-term dipeptidyl peptidase 4 inhibition worsens hypertension and renal and cardiac abnormalities in obese SHHF rats. 2021. Journal of the American Heart Association 10:e020088, 2021. Open Access, PMID: 33682436
 
Jeske, S.S., Brand, M., Ziebart, A., Laban, S., Doescher, J., Greve, J., Jackson, E.K.,  Hoffmann, T.K., Brunner, C., and Schuler, P.J.:  Adenosine-producing regulatory B cells in head and neck cancer.  Cancer Immunology Immunotherapy 69: 1205-1216, 2020. PMC7303082, Open Access, PMID: 32146518

Jin K, Ma Y, Manrique-Caballero CL, Li H, Emlet DR, Li S, Baty CJ, Wen X, Kim-Campbell N, Frank A, Menchikova EV, Pastor-Soler NM, Hallows KR, Jackson EK, Shiva S, Pinsky MR, Zuckerbraun BS, Kellum JA, Gómez H. Activation of AMP-activated protein kinase during sepsis/inflammation improves survival by preserving cellular metabolic fitness. FASEB J. 2020 Apr 4. doi: 10.1096/fj.201901900R. [Epub ahead of print] PMID: 32246808; PMC Journal - In Process

Jobbagy S, Vitturi DA, Salvatore SR, Pires MF, Rowart P, Emlet DR, Ross M, Hahn S, St Croix C, Wendell SG, Subramanya AR, Straub AC, Tan RJ, Schopfer FJ. Nrf2 activation protects against lithium-induced nephrogenic diabetes insipidus. JCI Insight. 2020 Jan 16;5(1). pii: 128578. doi: 10.1172/jci.insight.128578. PMID: 31941842; PMCID: PMC7030822

Kinsman BJ, Simmonds SS, Browning KN, Wenner MM, Farquhar WB, Stocker SD. Integration of Hypernatremia and Angiotensin II by the Organum Vasculosum of the Lamina Terminalis Regulates Thirst. J Neurosci. 2020 Mar 4;40(10):2069-2079. doi: 10.1523/JNEUROSCI.2208-19.2020. Epub 2020 Jan 31. PMID: 32005766.

Kleyman TR, Eaton DC. Regulating ENaC's gate. Am J Physiol Cell Physiol. 2020 Jan 1;318(1):C150-C162. doi: 10.1152/ajpcell.00418.2019. PMID: 31721612; PMCID: PMC6985836 [Available on 2021-01-01]

Long KR, Rbaibi Y, Gliozzi ML, Ren Q, Weisz OA. Differential kidney proximal tubule cell responses to protein overload by albumin and its ligands. Am J Physiol Renal Physiol. 2020 Mar 1;318(3):F851-F859. doi: 10.1152/ajprenal.00490.2019. PMID: 32068462; PMCID: PMC7099508 [Available on 2021-03-01]

Ludwig, N., Yerneni, S.S., Menshikova, E.V., Gillespie, D.G., Jackson, E.K., and Whiteside, T.L. Simultaneous inhibition of glycolysis and oxidative phosphorylation triggers a multi-fold increase in secretion of exosomes: possible role of 2’3’-cAMP, Scientific Reports, 2020 (In Press). Open Access

Ludwig, N., Azambuja, J.H., Rao, A., Gillespie, D.G., Jackson, E.K., and Whiteside, T.L. Adenosine receptors regulate exosome secretion.  Purinergic Signaling 16: 231-240, 2020. PMC7367962, PMID: 32440820
 
Ludwig, N., Gillespie, D.G., Reichert T.E., Jackson, E.K., and Whiteside, T.L.:  Purine metabolites in tumor-derived exosomes may facilitate immune escape of head and neck squamous cell carcinoma.  Cancers 12: 1602, 2020. PMC7352909, Open Access, PMID: 32560461
 
Ludwig, N., Yerneni, S.S., Azambuja, J.H., Gillespie, D.G., Menshikova, E.V., Jackson, E.K., and Whiteside, T.L.  Tumor-derived exosomes promote angiogenesis via adenosine A2B receptor signaling.  Angiogenesis 23: 599-610, 2020. PMC7529853, Open Access, PMID: 32419057

Park HJ, Fan Z, Bai Y, Ren Q, Rbaibi Y, Long KR, Gliozzi ML, Rittenhouse N, Locker JD, Poholek AC, Weisz OA. Transcriptional Programs Driving Shear Stress- Induced Differentiation of Kidney Proximal Tubule Cells in Culture. Front Physiol. 2020 Oct 30;11:587358. doi: 10.3389/fphys.2020.587358. PMID: 33192601; PMCID: PMC7662153.

Pitzer AL, Van Beusecum JP, Kleyman TR, Kirabo A. ENaC in Salt-Sensitive Hypertension: Kidney and Beyond. Curr Hypertens Rep. 2020 Aug 27;22(9):69. PMID: 32852643 Free PMC article. Review. PMCID: PMC7452925
 
Ponzoni L, Nguyen NH, Bahar I, Brodsky JL. Complementary computational and experimental evaluation of missense variants in the ROMK potassium channel. PLoS Comput Biol. 2020 Apr 6;16(4). e1007749. doi: 10.1371/journal.pcbi.1007749. PMID: 32251469; PMCID: PMC7162551

Rein JL, Heja S, Flores D, Carrisoza-Gaytán R, Lin NYC, Homan KA, Lewis JA, Satlin LM. Effect of luminal flow on doming of mpkCCD cells in a 3D perfusable kidney cortical collecting duct model. Am J Physiol Cell Physiol. 2020 Jul 1;319(1):C136-C147. doi: 10.1152/ajpcell.00405.2019. Epub 2020 May 13. PMID: 32401606; PMCID: PMC7468887.
 
Ren Q, Weyer K, Rbaibi Y, Long KR, Tan RJ, Nielsen R, Ilsö Christenssen E, Baty CJ, Kashlan OB, Weisz OA. Distinct functions of megalin and cubilin receptors in recovery of normal and nephrotic levels of filtered albumin. Am J Physiol Renal Physiol. 2020 Mar 23. doi: 10.1152/ajprenal.00030.2020. [Epub ahead of print] PMID: 32200668; “PMC Journal - In Process”

Sander V, Przepiorski A, Crunk AE, Hukriede NA, Holm TM, Davidson AJ. Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells. STAR Protoc. 2020 Oct 29;1(3):100150. doi: 10.1016/j.xpro.2020.100150. PMID: 33377044; PMCID: PMC7757290.
 
Schlesinger PH, Blair HC, Beer Stolz D, Riazanski V, Ray EC, Tourkova IL, Nelson DJ. Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport. Am J Physiol Cell Physiol. 2020 Jan 1;318(1):C111-C124. doi: 10.1152/ajpcell.00120.2019. PMID: 31532718; PMCID: PMC6985832 [Available on 2021-01-01]

Shi S, Montalbetti N, Wang X, Rush BM, Marciszyn AL, Baty CJ, Tan RJ, Carattino MD, Kleyman TR. Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel. J Biol Chem. 2020 Feb 20. pii: jbc.RA119.011789. doi: 10.1074/jbc.RA119.011789. PMID: 32079677; “PMC Journal - In Process”

Shipman KE, Weisz OA. Making a Dent in Dent Disease. Function (Oxf). 2020;1(2):zqaa017. doi: 10.1093/function/zqaa017. Epub 2020 Sep 11. PMID: 33015630. PMCID: PMC7519470

Singh A, Vashistha N, Heck J, Tang X, Wipf P, Brodsky JL, Hampton RY. Direct involvement of Hsp70 ATP hydrolysis in Ubr1-dependent quality control. Mol Biol Cell. 2020 Nov 15; 31(24):2669-86. PMID: 32966159 PMCID: PMC7927186

Terrab L, Rosenker CJ, Johnstone L, Ngo LK, Zhang L, Ware NF, Miller B, Topacio AZ, Sannino S, Brodsky JL, Wipf P. Synthesis and Selective Functionalization of Thiadiazine 1,1-Dioxides with Efficacy in a Model of Huntington's Disease. ACS Med Chem Lett. 2020 Feb 20; 11(5):984-90. doi: 10.1021/acsmedchemlett.0c00018. eCollection 2020 May 14.  PMID: 32435415 PMCID: PMC7236266

Tillman TS, Choi Z, Xu Y, Tang P. Functional Tolerance to Cysteine Mutations in Human α7 Nicotinic Acetylcholine Receptors. ACS Chem Neurosci. 2020 Feb 5;11(3):242-247. doi: 10.1021/acschemneuro.9b00647. PMID: 31951367; PMCID: PMC7057392 [Available on 2021-02-05]

Tilstra JS, John S, Gordon RA, Leibler C, Kashgarian M, Bastacky S, Nickerson KM, Shlomchik MJ. B cell-intrinsic TLR9 expression is protective in murine lupus. J Clin Invest. 2020 Mar 19:132328. doi: 10.1172/JCI132328. Epub ahead of print. PMID: 32191633.

Wen X, Li S, Frank A, Chen X, Emlet D, Hukriede NA, Kellum JA. Time-dependent effects of histone deacetylase inhibition in sepsis-associated acute kidney injury. Intensive Care Med Exp. 2020 Feb 7;8(1):9. doi: 10.1186/s40635-020-0297-3. PMID: 32034542; PMCID: PMC7007462




2021 

Publications 2021:

Al-Bataineh MM, Kinlough CL, Mi Z, Jackson EK, Mutchler SM, Emlet DR, Kellum JA, Hughey RP. KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2021 Aug 1;321(2):F135-F148. PMID: 34151589 PMCID: PMC8424662
 
Bhatia, T.N., R.N. Clark, P.G. Needham, K.M. Miner, A.S. Jamenis, E.A. Eckhoff, N. Abraham, X. Hu, P. Wipf, K.C. Luk, J.L. Brodsky, and R.K. Leak. Heat Shock Protein 70 as a Sex-Skewed Regulator of α-Synucleinopathy. Neurotherapetics (2021) 18: 2541-2564. PMID: 34528172 PMCID: PMC8804008
 
Birder LA, Jackson EK. Adv Geriatr Med Res. Dysregulated Purine Metabolism Contributes to Age-Associated Lower Urinary Tract Dysfunctions. 2021;3(4):e210018. doi: 10.20900/agmr20210018. Epub 2021 Sep 29. PMID: 34676378 Free PMC article.
 
Burns, G.D., O. Hillal, Z. Sun, K.R. Reutter, G.M. Preston, A.A. Augustine, J.L. Brodsky, and C.J. Guerriero. Distinct classes of misfolded proteins differentially affect the growth of yeast compromised for proteasome function. FEBS Lett. 2021 Sep;595(18):2383-2394. PMID: 34358326 PMCID: PMC8531709
 
Chiba T, Cerqueira DM, Li Y, Bodnar AJ, Mukherjee E, Pfister K, Phua YL, Shaikh K, Sanders BT, Hemker SL, Pagano PJ, Wu YL, Ho J, Sims-Lucas S. Endothelial-Derived miR-1792 Promotes Angiogenesis to Protect against Renal Ischemia-Reperfusion Injury. J Am Soc Nephrol. 2021 Mar;32(3):553-562. doi: 10.1681/ASN.2020050717. Epub 2021 Jan 29. PMID: 33514560; PMCID: PMC7920169.
 
Dalghi MG, Ruiz WG, Clayton DR, Montalbetti N, Daugherty SL, Beckel JM, Carattino MD, Apodaca G. Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception. JCI Insight. 2021 Oct 8;6(19):e152984. PMID: 34464353 PMCID: PMC8525643
 
Elijovich F, Kleyman TR, Laffer CL, Kirabo A. Immune Mechanisms of Dietary Salt-Induced Hypertension and Kidney Disease: Harry Goldblatt Award for Early Career Investigators 2020. Hypertension. 2021 Aug;78(2):252-260. PMID: 34232678 PMCID: PMC8437138
 
Fazzari M, Woodcock SR, Rowart P, Ricart K, Lancaster JR Jr, Patel R, Vitturi DA, Freeman BA, Schopfer FJ. Endogenous generation of nitro-fatty acid hybrids having dual nitrate ester (RONO2) and nitroalkene (RNO2) substituents. Redox Biol. 2021; 41:101913. doi: 10.1016/j.redox.2021.101913. PMC Journal - In Process
 
Frindt G, Shi S, Kleyman TR, Palmer LG. Cleavage state of γENaC in mouse and rat kidneys. Am J Physiol Renal Physiol. 2021 Mar 1;320(3):F485-F491. Epub 2021 Feb 1. PMID: 33522411. PMCID: PMC7988816 (available on 2022-03-01)
 
Fu H, Gui Y, Liu S, Wang Y, Bastacky SI, Qiao Y, Zhang R, Bonin C, Hargis G, Yu Y, Kreutzer DL, Biswas PS, Zhou Y, Wang Y, Tian XJ, Liu Y, Zhou D. The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment. iScience. 2021 Sep 9;24(10):103112. doi: 10.1016/j.isci.2021.103112. PMID: 34622165; PMCID: PMC8479790.
 
Goeckeler-Fried, J.L., R.A. Denny, D. Joshi, C. Hill, M.B. Larsen, A.N. Chiang, R.A. Frizzell, P. Wipf, E.J. Sorscher, and J.L. Brodsky. Improved correction of F508del-CFTR biogenesis with a folding facilitator and an inhibitor of protein ubiquitination. Bioorg. Med. Chem. Lettr. (2021) 48: 128243. PMID: 34246753 PMCID: PMC8869796
 
Hixson EA, Borker PV, Jackson EK, Macatangay BJ. The Adenosine Pathway and Human Immunodeficiency Virus-Associated Inflammation. Open Forum Infect Dis. 2021 Jul 24;8(9):ofab396. doi: 10.1093/ofid/ofab396. eCollection 2021 Sep. PMID: 34557556 Free PMC article.s
 
Honer, J., K.M. Niemeyer, C. Fercher, A.L. Diez Tissera, N. Jaberolansar, Y. Jafrani, C. Zhou, J.J. Caramelo, A.M. Shewan, B.L. Schulz, J.L. Brodsky, and L.F. Zacchi. TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis. Biochim. Biophys. Acta Mol. Cell. Res. (2021) 1868: 119073. PMID: 34062155 PMCID: PMC8889903
 
Jackson, E.K., Mi, Z, Gillespie, D.G., and Tofovic, S.P.:  Long-term dipeptidyl peptidase 4 inhibition worsens hypertension and renal and cardiac abnormalities in obese SHHF rats. 2021. Journal of the American Heart Association 10:e020088, 2021. Open Access, PMID: 33682436
 
Levin-Schwartz Y, Curtin P, Flores D, Aushev VN, Tamayo-Ortiz M, Svensson K, Pantic I, Estrada-Gutierrez G, Pizano-Zárate ML, Gennings C, Satlin LM, Baccarelli AA, Tellez-Rojo MM, Wright RO, Sanders AP. Exosomal miRNAs in urine associated with children's cardiorenal parameters: a cross-sectional study. Epigenomics. 2021 Apr;13(7):499-512. PMID: 33635093 PMCID: PMC8033423
 
Madhur MS, Elijovich F, Alexander MR, Pitzer A, Ishimwe J, Van Beusecum JP, Patrick DM, Smart CD, Kleyman TR, Kingery J, Peck RN, Laffer CL, Kirabo A. Hypertension: Do Inflammation and Immunity Hold the Key to Solving this Epidemic? Circ Res. 2021 Apr 2;128(7):908-933. Epub 2021 Apr 1. PMID: 33793336. PMCID: PMC8023750
 
 
Mandapathil M, Szczepanski MJ, Jackson EK, Lang S, Whiteside TL. Breast Cancer Cell-Derived Adenosine Enhances Generation and Suppressor Function of Human Adaptive Regulatory T Cells. J Pers Med. 2021 Jul 30;11(8):754. doi: 10.3390/jpm11080754. PMID: 34442398 Free PMC article.
 
Mihai MC, Popa MA, Șuică VI, Antohe F, Jackson EK, Leeners B, Simionescu M, Dubey RK. Proteomic Analysis of Estrogen-Mediated Enhancement of Mesenchymal Stem Cell-Induced Angiogenesis In Vivo. Cells. 2021 Aug 24;10(9):2181. doi: 10.3390/cells10092181. PMID: 34571830 Free PMC article.
 
Montalbetti N, Carattino MD. Acid-sensing ion channels modulate bladder nociception. Am J Physiol Renal Physiol. 2021 Nov 1;321(5):F587-F599. PMID: 34514879 PMCID: PMC8813206
 
Mutchler SM, Kirabo A, Kleyman TR. Epithelial Sodium Channel and Salt-Sensitive Hypertension. Hypertension. 2021. 77(3):759-767. PubMed PMID: 33486988; PubMed Central PMCID: PMC7878349; DOI: 10.1161/HYPERTENSIONAHA.120.14481.
 
Mutchler SM, Hasan M, Kohan DE, Kleyman TR, Tan RJ. Deletion of the Gamma Subunit of ENaC in Endothelial Cells Does Not Protect against Renal Ischemia Reperfusion Injury. Int J Mol Sci. 2021 Oct 9;22(20):10914. PMID: 34681576 PMCID: PMC8535410
 
Needham, P.J., C.A. Bertrand, J.L. Goeckeler-Fried, C. Zhang, Z. Sun, A.R. Wetzel, and J.L. Brodsky. SLC6A9 is selected for endoplasmic reticulum associated degradation (ERAD) due to Hsp70-dependent targeting of the STAS domain. Biochem. J. (2021) 478: 4203-4220. PMID: 34821356 PMCID: PMC8826537
 
Ramkumar N, Stuart D, Peterson CS, Hu C, Wheatley W, Cho JM, Symons JD, Kohan DE. Loss of Soluble (Pro)renin Receptor Attenuates Angiotensin-II Induced Hypertension and Renal Injury  Circ Res. 2021 Apr 23. doi: 10.1161/CIRCRESAHA.120.317532. Online ahead of print. PMID: 33890822 PMCID In process.
 
Ray EC, Carrisoza-Gaytan R, Al-Bataineh M, Marciszyn AL, Nkashama LJ, Chen J, Winfrey A, Griffiths S, Lam TR, Flores D, Wu P, Wang W, Huang CL, Subramanya AR, Kleyman TR, Satlin LM. L-WNK1 is required for BK channel activation in intercalated cells. Am J Physiol Renal Physiol. 2021 Aug 1;321(2):F245-F254. PMID: 34229479 PMCID: PMC8424664 (
 
Ray EC, Pitzer A, Lam T, Jordahl A, Patel R, Ao M, Marciszyn A, Winfrey A, Barak Y, Sheng S, Kirabo A, Kleyman TR. Salt sensitivity of volume and blood pressure in a mouse with globally reduced ENaC γ-subunit expression. Am J Physiol Renal Physiol. 2021 Dec 1;321(6):F705-F714. PMID: 34632813 PMCID: PMC8714976
 
Rosselli M, Cometti BPS, Leeners B, Szutkowska ME, Jackson EK, Dubey RK. Modulation of Cyclic AMP Levels in Fallopian Tube Cells by Natural and Environmental Estrogens. Cells. 2021 May 19;10(5):1250. doi: 10.3390/cells10051250. PMID: 34069403 Free PMC article.
 
Sahinoz M, Elijovich F, Ertuglu LA, Ishimwe J, Pitzer A, Saleem M, Mwesigwa N, Kleyman TR, Laffer CL, Kirabo A. Salt Sensitivity of Blood Pressure in Blacks and Women: A Role of Inflammation, Oxidative Stress, and Epithelial Na+ Channel. Antioxid Redox Signal. 2021 Dec 20;35(18):1477-1493. PMID: 34569287 PMCID: PMC8713266
 
Sannino, S., M.E. Yates, M.E. Schurdak, S. Oesterreich, A.V. Lee, P. Wipf, and J.L. Brodsky. Unique integrated stress response sensors regulate cancer cell susceptibility when Hsp70 activity is compromised. eLife (2021) 10: e64977. PMID: 34180400 PMCID: PMC8275131
Sun, Z., C.J. Guerriero, and J.L. Brodsky. Substrate Ubiquitination Retains Misfolded Membrane Proteins in the Endoplasmic Reticulum for Degradation. Cell Rep. (2021) 36: 109717. PMID: 34551305 PMCID: PMC8503845
 
Theodoraki MN, Laban S, Jackson EK, Lotfi R, Schuler PJ, Brunner C, Hoffmann TK, Whiteside TL, Hofmann L. Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients. Br J Cancer. 2021 Dec;125(12):1677-1686. doi: 10.1038/s41416-021-01567-8. Epub 2021 Oct 12. PMID: 34642463 Free PMC article.
 
Weisz OA. Considerations in Comparing Proximal Tubule Cell Culture Models. J Am Soc Nephrol. 2021 Aug;32(8):2099-2100. PMID: 34253588  PMCID: PMC8455277
 
Weisz OA. Endocytic adaptation to functional demand by the kidney proximal tubule. J Physiol. 2021 Jul;599(14):3437-3446. PMID: 34036593  PMCID: PMC8715547 
 
 
 
 


2022 

Publications 2022:

Airik M, McCourt B, Ozturk TT, Huynh AB, Zhang X, Tometich JT, Topaloglu R, Ozen H, Orhan D, Nejak-Bowen K, Monga SP, Hand TW, Ozaltin F, Airik R. Mitigation of portal fibrosis and cholestatic liver disease in ANKS6-deficient livers by macrophage depletion. FASEB J. 2022 February;36(2):e22157. PubMed PMID: 35032404; PubMed Central PMCID: PMC8852242
 
Al-Bataineh MM, Kinlough CL, Mi Z, Jackson EK, Mutchler SM, Emlet DR, Kellum JA, Hughey RP. KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2021 Aug 1;321(2):F135-F148. PMID: 34151589 PMCID: PMC8424662
 
Bhatia, T.N., R.N. Clark, P.G. Needham, K.M. Miner, A.S. Jamenis, E.A. Eckhoff, N. Abraham, X. Hu, P. Wipf, K.C. Luk, J.L. Brodsky, and R.K. Leak. Heat Shock Protein 70 as a Sex-Skewed Regulator of α-Synucleinopathy. Neurotherapetics (2021) 18: 2541-2564. PMID: 34528172 PMCID: PMC8804008
 
Birder LA, Jackson EK. Adv Geriatr Med Res. Dysregulated Purine Metabolism Contributes to Age-Associated Lower Urinary Tract Dysfunctions. Adv Geriatr Med Res 2021;3(4):e210018. doi: 10.20900/agmr20210018. Epub 2021 Sep 29. PMID: 34676378 Free PMC article.
 
Burns, G.D., O. Hillal, Z. Sun, K.R. Reutter, G.M. Preston, A.A. Augustine, J.L. Brodsky, and C.J. Guerriero. Distinct classes of misfolded proteins differentially affect the growth of yeast compromised for proteasome function. FEBS Lett. 2021 Sep;595(18):2383-2394. PMID: 34358326 PMCID: PMC8531709
 
Dalghi MG, Ruiz WG, Clayton DR, Montalbetti N, Daugherty SL, Beckel JM, Carattino MD, Apodaca G. Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception. JCI Insight. 2021 Oct 8;6(19):e152984. PMID: 34464353 PMCID: PMC8525643
 
Edwards A, Long KR, Baty CJ, Shipman KE, Weisz OA. Modelling normal and nephrotic axial uptake of albumin and other filtered proteins along the proximal tubule. J Physiol. 2022 Apr;600(8):1933-1952. PMID: 35178707 PMCID: PMC9012691
 
Elijovich F, Kleyman TR, Laffer CL, Kirabo A. Immune Mechanisms of Dietary Salt-Induced Hypertension and Kidney Disease: Harry Goldblatt Award for Early Career Investigators 2020. Hypertension. 2021 Aug;78(2):252-260. PMID: 34232678 PMCID: PMC8437138
 
Fu H, Gui Y, Liu S, Wang Y, Bastacky SI, Qiao Y, Zhang R, Bonin C, Hargis G, Yu Y, Kreutzer DL, Biswas PS, Zhou Y, Wang Y, Tian XJ, Liu Y, Zhou D. The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment. iScience. 2021 Sep 9;24(10):103112. doi: 10.1016/j.isci.2021.103112. PMID: 34622165; PMCID: PMC8479790.
 
Galley, J.C., Hahn, S.A., Miller, M.P., Durgin, B.G., Jackson, E.K., Stocker, S.D., and Straub, A.C.  Angiotensin II augments renal vascular smooth muscle sGC expression via an AT1R-FoxO transcription factor signaling axis.  British Journal of Pharmacology, 2022 Jun;179(11):2490-2504 PMID: 33963547  PMCID: PMC8883839
 
Galvin, J., E. Curran, F. Arteaga, A. Goossens, N. Aubuchon-Endsley, M.A. McMurray, J. Moore, K.C. Hansen, H. Chial, H. Potter, J.L. Brodsky, and C.M. Coughlan. Proteasome activity modulates amyloid toxicity. FEMS Yeast Res. (2022) 22: 1-13. PMID: 35150241 PMCID: PMC8906389
 
Goeckeler-Fried, J.L., R.A. Denny, D. Joshi, C. Hill, M.B. Larsen, A.N. Chiang, R.A. Frizzell, P. Wipf, E.J. Sorscher, and J.L. Brodsky. Improved correction of F508del-CFTR biogenesis with a folding facilitator and an inhibitor of protein ubiquitination. Bioorg. Med. Chem. Lettr. (2021) 48: 128243. PMID: 34246753 PMCID: PMC8869796
 
Gordon RA, Giannouli C, Raparia C, Bastacky SI, Marinov A, Hawse W, Cattley R, Tilstra JS, Campbell AM, Nickerson KM, Davidson A, Shlomchik MJ. Rubicon promotes rather than restricts murine lupus and is not required for LC3-associated phagocytosis. JCI Insight. 2022 Apr 8;7(7):e155537. doi: 10.1172/jci.insight.155537. PMID: 35192551; PMCID: PMC9057630.
 
Hixson EA, Borker PV, Jackson EK, Macatangay BJ. The Adenosine Pathway and Human Immunodeficiency Virus-Associated Inflammation. Open Forum Infect Dis. 2021 Jul 24;8(9):ofab396. doi: 10.1093/ofid/ofab396. eCollection 2021 Sep. PMID: 34557556 Free PMC article.
 
Honer, J., K.M. Niemeyer, C. Fercher, A.L. Diez Tissera, N. Jaberolansar, Y. Jafrani, C. Zhou, J.J. Caramelo, A.M. Shewan, B.L. Schulz, J.L. Brodsky, and L.F. Zacchi. TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis. Biochim. Biophys. Acta Mol. Cell. Res. (2021) 1868: 119073. PMID: 34062155 PMCID: PMC8889903
 
Levin-Schwartz Y, Curtin P, Flores D, Aushev VN, Tamayo-Ortiz M, Svensson K, Pantic I, Estrada-Gutierrez G, Pizano-Zárate ML, Gennings C, Satlin LM, Baccarelli AA, Tellez-Rojo MM, Wright RO, Sanders AP. Exosomal miRNAs in urine associated with children's cardiorenal parameters: a cross-sectional study. Epigenomics. 2021 Apr;13(7):499-512. PMID: 33635093 PMCID: PMC8033423
 
Long K, Vaughn Z, McDaniels MD, Joyasawal S, Przepiorski A, Parasky E, Sander V, Close D, Johnston PA, Davidson AJ, de Caestecker M, Hukriede NA, Huryn DM. Validation of HDAC8 Inhibitors as Drug Discovery Starting Points to Treat Acute Kidney Injury. ACS Pharmacol Transl Sci. 2022 Mar 16;5(4):207-215. PMID: 35434532 PMCID: PMC9003639
 
Long KR, Rbaibi Y, Bondi CD, Ford BR, Poholek AC, Boyd-Shiwarski CR, Tan RJ, Locker JD, Weisz OA. Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells. Am J Physiol Renal Physiol. 2022 Jan 1;322(1):F14-F26. PMID: 34747197 PMCID: PMC8698540
 
Mandapathil M, Szczepanski MJ, Jackson EK, Lang S, Whiteside TL. Breast Cancer Cell-Derived Adenosine Enhances Generation and Suppressor Function of Human Adaptive Regulatory T Cells. J Pers Med. 2021 Jul 30;11(8):754. doi: 10.3390/jpm11080754. PMID: 34442398 Free PMC article.
 
Mihai MC, Popa MA, Șuică VI, Antohe F, Jackson EK, Leeners B, Simionescu M, Dubey RK. Proteomic Analysis of Estrogen-Mediated Enhancement of Mesenchymal Stem Cell-Induced Angiogenesis In Vivo. Cells. 2021 Aug 24;10(9):2181. doi: 10.3390/cells10092181. PMID: 34571830 Free PMC article.
 
Montalbetti N, Carattino MD. Acid-sensing ion channels modulate bladder nociception. Am J Physiol Renal Physiol. 2021 Nov 1;321(5):F587-F599. PMID: 34514879 PMCID: PMC8813206
 
Montalbetti N, Dalghi MG, Bastacky SI, Clayton DR, Ruiz WG, Apodaca G, Carattino MD. Bladder infection with uropathogenic Escherichia coli increases the excitability of afferent neurons. Am J Physiol Renal Physiol. 2022 Jan 1;322(1):F1-F13. PMID: 34779263 PMCID: PMC8698541
 
Mutchler SM, Hasan M, Kohan DE, Kleyman TR, Tan RJ. Deletion of the Gamma Subunit of ENaC in Endothelial Cells Does Not Protect against Renal Ischemia Reperfusion Injury. Int J Mol Sci. 2021 Oct 9;22(20):10914. PMID: 34681576 PMCID: PMC8535410
 
Mutchler SM, Kleyman TR. Effects of amiloride on acetylcholine-dependent arterial vasodilation evolve over time in mice on a high salt diet. Physiol Rep. 2022 Apr;10(7):e15255. PMID: 35384364 PMCID: PMC8984245
 
Needham, P.J., C.A. Bertrand, J.L. Goeckeler-Fried, C. Zhang, Z. Sun, A.R. Wetzel, and J.L. Brodsky. SLC6A9 is selected for endoplasmic reticulum associated degradation (ERAD) due to Hsp70-dependent targeting of the STAS domain. Biochem. J. (2021) 478: 4203-4220. PMID: 34821356 PMCID: PMC8826537
 
Pacini ESA, Satori NA, Jackson EK, Godinho RO. Front Immunol. Extracellular cAMP-Adenosine Pathway Signaling: A Potential Therapeutic Target in Chronic Inflammatory Airway Diseases. 2022 Apr 11;13:866097. doi: 10.3389/fimmu.2022.866097. eCollection 2022. PMID: 35479074 Free PMC article. 
 
Porter AW, Nguyen DN, Clayton DR, Ruiz WG, Mutchler SM, Ray EC, Marciszyn AL, Nkashama LJ, Subramanya AR, Gingras S, Kleyman TR, Apodaca G, Hendershot LM, Brodsky JL, Buck TM. The molecular chaperone GRP170 protects against ER stress and acute kidney injury in mice. JCI Insight. 2022 Mar 8;7(5):e151869. PMID: 35104250 PMCID: PMC8983141
 
Przepiorski A, Vanichapol T, Espiritu EB, Crunk AE, Parasky E, McDaniels MD, Emlet DR, Salisbury R, Happ CL, Vernetti LA, MacDonald ML, Kellum JA, Kleyman TR, Baty CJ, Davidson AJ, Hukriede NA. Modeling oxidative injury response in human kidney organoids. Stem Cell Res Ther. 2022 Feb 21;13(1):76. PMID: 35189973 PMCID: PMC8862571
 
Ray EC, Carrisoza-Gaytan R, Al-Bataineh M, Marciszyn AL, Nkashama LJ, Chen J, Winfrey A, Griffiths S, Lam TR, Flores D, Wu P, Wang W, Huang CL, Subramanya AR, Kleyman TR, Satlin LM. L-WNK1 is required for BK channel activation in intercalated cells. Am J Physiol Renal Physiol. 2021 Aug 1;321(2):F245-F254. PMID: 34229479 PMCID: PMC8424664 (
 
Ray EC, Pitzer A, Lam T, Jordahl A, Patel R, Ao M, Marciszyn A, Winfrey A, Barak Y, Sheng S, Kirabo A, Kleyman TR. Salt sensitivity of volume and blood pressure in a mouse with globally reduced ENaC γ-subunit expression. Am J Physiol Renal Physiol. 2021 Dec 1;321(6):F705-F714. PMID: 34632813 PMCID: PMC8714976
 
Rosselli M, Cometti BPS, Leeners B, Szutkowska ME, Jackson EK, Dubey RK. Modulation of Cyclic AMP Levels in Fallopian Tube Cells by Natural and Environmental Estrogens. Cells. 2021 May 19;10(5):1250. doi: 10.3390/cells10051250. PMID: 34069403 Free PMC article.
 
Sahinoz M, Elijovich F, Ertuglu LA, Ishimwe J, Pitzer A, Saleem M, Mwesigwa N, Kleyman TR, Laffer CL, Kirabo A. Salt Sensitivity of Blood Pressure in Blacks and Women: A Role of Inflammation, Oxidative Stress, and Epithelial Na+ Channel. Antioxid Redox Signal. 2021 Dec 20;35(18):1477-1493. PMID: 34569287 PMCID: PMC8713266
 
Sannino, S., M.E. Yates, M.E. Schurdak, S. Oesterreich, A.V. Lee, P. Wipf, and J.L. Brodsky. Unique integrated stress response sensors regulate cancer cell susceptibility when Hsp70 activity is compromised. eLife (2021) 10: e64977. PMID: 34180400 PMCID: PMC8275131
 
Shi, S., T.M. Buck, A.J. Nickerson, J.L. Brodsky, and T.R. Kleyman. Paraoxonase 2 is an ER chaperone that regulates expression of the epithelial sodium channel. Am. J. Phys. Cell Physiol. (2022) 322: C111-C121. PMID: 34852210 PMCID: PMC8759969  
 
Sun, Z., C.J. Guerriero, and J.L. Brodsky. Substrate Ubiquitination Retains Misfolded Membrane Proteins in the Endoplasmic Reticulum for Degradation. Cell Rep. (2021) 36: 109717. PMID: 34551305 PMCID: PMC8503845
 
Theodoraki MN, Laban S, Jackson EK, Lotfi R, Schuler PJ, Brunner C, Hoffmann TK, Whiteside TL, Hofmann L. Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients. Br J Cancer. 2021 Dec;125(12):1677-1686. doi: 10.1038/s41416-021-01567-8. Epub 2021 Oct 12. PMID: 34642463 Free PMC article.
 
Wang XP, Balchak DM, Gentilcore C, Clark NL, Kashlan OB. Activation by cleavage of the epithelial Na+ channel α and γ subunits independently coevolved with the vertebrate terrestrial migration. Elife. 2022 Jan 5;11:e75796. PMID: 34984981 PMCID: PMC8791634
 
Weisz OA. Considerations in Comparing Proximal Tubule Cell Culture Models. J Am Soc Nephrol. 2021 Aug;32(8):2099-2100. PMID: 34253588  PMCID: PMC8455277
 
Weisz OA. Endocytic adaptation to functional demand by the kidney proximal tubule. J Physiol. 2021 Jul;599(14):3437-3446. PMID: 34036593  PMCID: PMC8715547 
 
Zhang L, Wang X, Chen J, Kleyman TR, Sheng S. Accessibility of ENaC extracellular domain central core residues. J Biol Chem. 2022 Mar 23;298(5):101860. PMID: 35339489  PMCID: PMC905216
  
 
 

2023 

Publications 2023:

Brodsky JL, Engelman DM, Hendershot LM, Piana-Agostinetti S, Sommer T. Taking out the trash: How misfolded proteins are removed from the endoplasmic reticulum. Faculty reviews. 2022;11:29. PubMed PMID: 36267301; PubMed Central PMCID: PMC9554898; DOI: 10.12703/r-01-0000018.
 
Shipman KE, Long KR, Cowan IA, Rbaibi Y, Baty CJ, Weisz OA. An Adaptable Physiological Model of Endocytic Megalin Trafficking in Opossum Kidney Cells and Mouse Kidney ProximalTubule. Function (Oxford, England). 2022;3(6):zqac046. PubMed PMID: 36325513; PubMed Central PMCID: PMC9614980; DOI: 10.1093/function/zqac046.
 
Ikeda Y, Zabbarova I, de Rijk M, Kanai A, Wolf-Johnston A, Weiss JP, Jackson E, Birder L. Effects of vasopressin receptor agonists on detrusor smooth muscle tone in young and aged bladders: Implications for nocturia treatment. Continence (Amsterdam, Netherlands). 2022 June;2. PubMed PMID: 35789681; PubMed Central PMCID: PMC9250757; DOI: 10.1016/j.cont.2022.100032.
 
 
Jackson EK, Menshikova EV, Ritov VB, Mi Z, Birder LA. 8-Aminoinosine and 8-Aminohypoxanthine Inhibit Purine Nucleoside Phosphorylase and Exert Diuretic and
Natriuretic Activity. The Journal of pharmacology and experimental therapeutics. 2022 August;382(2):135-148. PubMed PMID: 35609923; PubMed Central PMCID: PMC9639651; DOI: 10.1124/jpet.122.001221.
 
Pitzer A, Elijovich F, Laffer CL, Ertuglu LA, Sahinoz M, Saleem M, Krishnan J, Dola T, Aden LA, Sheng Q, Raddatz MA, Wanjalla C, Pakala S, Davies SS, Patrick DM, Kon V, Ikizler TA, Kleyman T, Kirabo A. DC ENaC-Dependent Inflammasome Activation Contributes to Salt-Sensitive Hypertension. Circulation research. 2022 August 5;131(4):328-344. PubMed PMID:35862128; PubMed Central PMCID: PMC9357159; DOI: 10.1161/CIRCRESAHA.122.320818.
 
Shao H, Taguwa S, Gilbert L, Shkedi A, Sannino S, Guerriero CJ, Gale-Day ZJ, Young ZT, Brodsky JL, Weissman J, Gestwicki JE, Frydman J. A campaign targeting a conserved Hsp70 binding site uncovers how subcellular localization is linked to distinct biological activities. Cell chemical biology. 2022 August 18;29(8):1303-1316.e3. PubMed PMID: 35830852; PubMed Central PMCID: PMC9513760; DOI: 10.1016/j.chembiol.2022.06.006.
 
Clayton DR, Ruiz WG, Dalghi MG, Montalbetti N, Carattino MD, Apodaca G. Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts. American journal of physiology. Renal physiology. 2022 September 1;323(3):F299-F321. PubMed PMID: 35834272; PubMed Central PMCID:PMC9394772; DOI: 10.1152/ajprenal.00135.2022.
 
Bondi CD, Rush BM, Hartman HL, Wang J, Al-Bataineh MM, Hughey RP, Tan RJ. Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury. Antioxidants (Basel, Switzerland). 2022 September 14;11(9). PubMed PMID: 36139884; PubMed Central PMCID: PMC9495756; DOI: 10.3390/antiox11091810.
 
Carattino MD, Ruiz WG, Apodaca G. Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells. Journal of visualized experiments : JoVE. 2022 September 28(187). PubMed PMID: 36279534; PubMed Central PMCID: PMC10069332; DOI:10.3791/64532.
 
Montalbetti N, Przepiorski AJ, Shi S, Sheng S, Baty CJ, Maggiore JC, Carattino MD, Vanichapol T, Davidson AJ, Hukriede NA, Kleyman TR. Functional characterization of ion channels expressed in kidney organoids derived from human induced pluripotent stem cells. American journal of physiology. Renal physiology. 2022 October 1;323(4):F479-F491. PubMed PMID: 35979965; PubMed Central PMCID: PMC9529267; DOI:10.1152/ajprenal.00365.2021.
 
Ruiz WG, Clayton DR, Dalghi MG, Montalbetti N, Carattino MD, Apodaca G. Expression of Transgenes in Native Bladder Urothelium using Adenovirus-Mediated Transduction. Journal of visualized experiments : JoVE. 2022 October 6(188). PubMed PMID: 36282713; PubMed Central PMCID: PMC10108903; DOI: 10.3791/64584.
 
Jackson EK, Kitsios GD, Lu MY, Schaefer CM, Kessinger CJ, McVerry BJ, Morris A, Macatangay BJC. Suppressed renoprotective purines in COVID-19 patients with acute kidney injury. Scientific reports. 2022 October 17;12(1):17353. PubMed PMID: 36253495; PubMed Central PMCID: PMC9574168; DOI: 10.1038/s41598-022-22349-z.
 
Airik M, Phua YL, Huynh AB, McCourt BT, Rush BM, Tan RJ, Vockley J, Murray SL, Dorman A, Conlon PJ, Airik R. Persistent DNA damage underlies tubular cell polyploidization and progression to chronic kidney disease in kidneys deficient in the DNA repair protein FAN1. Kidney international. 2022 November;102(5):1042-1056. PubMed PMID: 35931300; PubMed Central PMCID: PMC9588672; DOI: 10.1016/j.kint.2022.07.003.
 
Birder LA, Jackson EK. Purine nucleoside phosphorylase as a target to treat age-associatedlower urinary tract dysfunction. Nature reviews. Urology. 2022 November;19(11):681-687. PubMed PMID: 36071153; PubMed Central PMCID: PMC9842101; DOI: 10.1038/s41585-022-
00642-w.
 
Blobner BM, Kirabo A, Kashlan OB, Sheng S, Arnett DK, Becker LC, Boerwinkle E, Carlson JC, Gao Y, Gibbs RA, He J, Irvin MR, Kardia SLR, Kelly TN, Kooperberg C, McGarvey ST, Menon VK, Montasser ME, Naseri T, Redline S, Reiner AP, Reupena MS, Smith JA, Sun X, Vaidya D, Viaud-Martinez KA, Weeks DE, Yanek LR, Zhu X, Minster RL, Kleyman TR. Rare Variants in Genes Encoding Subunits of the Epithelial Na(+) Channel Are Associated With Blood Pressure and Kidney Function. Hypertension (Dallas, Tex. : 1979). 2022 November;79(11):2573-2582. PubMed PMID: 36193739; PubMed Central PMCID: PMC9669116; DOI: 10.1161/HYPERTENSIONAHA.121.18513.
 
Wang XP, Tomilin V, Nickerson AJ, Tian R, Ertem M, McKernan A, Lei X, Pochynyuk O, Kashlan OB. Bile acids regulate the epithelial Na(+) channel in native tissues through direct binding at multiple sites. The Journal of physiology. 2022 November;600(21):4695-4711. PubMed PMID: 36071685; PubMed Central PMCID: PMC9633555; DOI: 10.1113/JP283318.
 
Boyd-Shiwarski CR, Shiwarski DJ, Griffiths SE, Beacham RT, Norrell L, Morrison DE, Wang J, Mann J, Tennant W, Anderson EN, Franks J, Calderon M, Connolly KA, Cheema MU, Weaver CJ, Nkashama LJ, Weckerly CC, Querry KE, Pandey UB, Donnelly CJ, Sun D, Rodan AR, Subramanya AR. WNK kinases sense molecular crowding and rescue cell volume via phase separation. Cell. 2022 November 23;185(24):4488-4506.e20. PubMed PMID: 36318922; PubMed Central PMCID: PMC9699283; DOI: 10.1016/j.cell.2022.09.042.
 
Porter AW, Brodsky JL, Buck TM. Emerging links between endoplasmic reticulum stress responses and acute kidney injury. American journal of physiology. Cell physiology. 2022 December 1;323(6):C1697-C1703. PubMed PMID: 36280391; PubMed Central PMCID: PMC9722262; DOI: 10.1152/ajpcell.00370.2022.
 
Ahmad T, Ertuglu LA, Masenga SK, Kleyman TR, Kirabo A. The epithelial sodium channel in inflammation and blood pressure modulation. Frontiers in cardiovascular medicine. 2023;10:1130148. PubMed PMID: 37123470; PubMed Central PMCID: PMC10132033; DOI:10.3389/fcvm.2023.1130148.
 
Mutchler SM, Shi S, Whelan SCM, Kleyman TR. Validation of commercially available antibodies directed against subunits of the epithelial Na(+) channel. Physiological reports. 2023 January;11(1):e15554. PubMed PMID: 36636010; PubMed Central PMCID: PMC9837423; DOI: 10.14814/phy2.15554.
 
Pitzer Mutchler A, Huynh L, Patel R, Lam T, Bain D, Jamison S, Kirabo A, Ray EC. The role of dietary magnesium deficiency in inflammatory hypertension. Frontiers in physiology. 2023;14:1167904. PubMed PMID: 37293263; PubMed Central PMCID: PMC10244581; DOI:10.3389/fphys.2023.1167904.
 
Su L, Zhang J, Gomez H, Kellum JA, Peng Z. Mitochondria ROS and mitophagy in acute kidney injury. Autophagy. 2023 February;19(2):401-414. PubMed PMID: 35678504; PubMed Central PMCID: PMC9851232; DOI: 10.1080/15548627.2022.2084862.
 
Dalghi MG, Montalbetti N, Wheeler TB, Apodaca G, Carattino MD. Real-Time Void Spot Assay. Journal of visualized experiments : JoVE. 2023 February 10(192). PubMed PMID: 36847378; PubMed Central PMCID: PMC10153432; DOI: 10.3791/64621.
 
Al-Bataineh MM, Kinlough CL, Marciszyn A, Lam T, Ye L, Kidd K, Maggiore JC, Poland PA, Kmoch S, Bleyer A, Bain DJ, Montalbetti N, Kleyman TR, Hughey RP, Ray EC. Influence of glycoprotein MUC1 on trafficking of the Ca(2+)-selective ion channels, TRPV5 and TRPV6 on in vivo calcium homeostasis. The Journal of biological chemistry. 2023. March;299(3):102925. PubMed PMID: 36682497; PubMed Central PMCID: PMC9996365; DOI: 10.1016/j.jbc.2023.102925.
 
Carrisoza-Gaytan R, Kroll KT, Hiratsuka K, Gupta NR, Morizane R, Lewis JA, Satlin LM. Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca(2+) signaling. American journal of physiology. Cell physiology. 2023 March 1;324(3):C757-C768. PubMed PMID: 36745528; PubMed Central PMCID: PMC10027089; DOI: 10.1152/ajpcell.00288.2022.
 
Nguyen NH, Brodsky JL. The cellular pathways that maintain the quality control and transport of diverse potassium channels. Biochimica et biophysica acta. Gene regulatory mechanisms. 2023 March;1866(1):194908. PubMed PMID: 36638864; PubMed Central PMCID: PMC9908860; DOI: 10.1016/j.bbagrm.2023.194908.
 
Zhang L, Wang X, Chen J, Sheng S, Kleyman TR. Extracellular intersubunit interactions modulate epithelial Na(+) channel gating. The Journal of biological chemistry. 2023
March;299(3):102914. PubMed PMID: 36649907; PubMed Central PMCID: PMC9975279;DOI: 10.1016/j.jbc.2023.102914.
 
Park JW, Okamoto LE, Kim SH, Baek SH, Sung JH, Jeon N, Gamboa A, Shibao CA, Diedrich A, Kim BJ, Biaggioni I. Use of Valsalva Maneuver to Detect Late-Onset Delayed Orthostatic Hypotension. Hypertension (Dallas, Tex. : 1979). 2023 April;80(4):792-801. PubMed PMID:36695176; PubMed Central PMCID: PMC10023507; DOI:
10.1161/HYPERTENSIONAHA.122.20098.
 
Shipman KE, Baty CJ, Long KR, Rbaibi Y, Cowan IA, Gerges M, Marciszyn AL, Kashlan OB, Tan RJ, Edwards A, Weisz OA. Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models. Journal of the American Society of Nephrology : JASN. 2023 April 1;34(4):619-640. PubMed PMID: 36758125; PubMed Central PMCID: PMC10103310; DOI: 10.1681/ASN.0000000000000084.
 
Gui Y, Wang Y, Palanza Z, Wang JL, Gupta P, Tao J, Qiao Y, Hargis G, Kreutzer DL, Bastacky SI, Yu Y, Wang Y, Liu S, Fu H, Zhou D. Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis. Molecular metabolism. 2023 May;71:101712. PubMed PMID:36963615; PubMed Central PMCID: PMC10090436; DOI: 10.1016/j.molmet.2023.101712.
 
Jackson EK, Gillespie DG, Mi Z. 8-Aminoguanine and Its Actions on Renal Excretory Function. Hypertension (Dallas, Tex. : 1979). 2023 May;80(5):981-994. PubMed PMID: 36802842; PubMed Central PMCID: PMC10112938; DOI: 10.1161/HYPERTENSIONAHA.122.20760.
 
Nickerson KM, Smita S, Hoehn KB, Marinov AD, Thomas KB, Kos JT, Yang Y, Bastacky SI, Watson CT, Kleinstein SH, Shlomchik MJ. Age-associated B cells are heterogeneous and dynamic drivers of autoimmunity in mice. The Journal of experimental medicine. 2023 May 1;220(5). PubMed PMID: 36828389; PubMed Central PMCID: PMC9997508; DOI:10.1084/jem.20221346.
 
Rbaibi Y, Long KR, Shipman KE, Ren Q, Baty CJ, Kashlan OB, Weisz OA. Megalin, cubilin, and Dab2 drive endocytic flux in kidney proximal tubule cells. Molecular biology of the cell. 2023 June 1;34(7):ar74. PubMed PMID: 37126375; PubMed Central PMCID: PMC10295476; DOI:
10.1091/mbc.E22-11-0510.
 
Sannino S, Manuel AM, Shang C, Wendell SG, Wipf P, Brodsky JL. Non-Essential Amino Acid Availability Influences Proteostasis and Breast Cancer Cell Survival During Proteotoxic Stress. Molecular cancer research : MCR. 2023 July 5;21(7):675-690. PubMed PMID: 36961392; PubMed Central PMCID: PMC10330057; DOI: 10.1158/1541-7786.MCR-22-0843.
 
Birder LA, Wolf-Johnston AS, Zabbarova I, Ikeda Y, Robertson AM, Cardozo R, Azari F, Kanai AJ, Kuchel GA, Jackson EK. Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling. The journals of gerontology. Series A, Biological sciences and medical sciences. 2023 July 18. PubMed PMID: 37463319; DOI:10.1093/gerona/glad171.
 
Kaverina N, Schweickart RA, Chan GC, Maggiore JC, Eng DG, Zeng Y, McKinzie SR, Perry HS, Ali A, O'Connor C, Pereira BMV, Theberge AB, Vaughan JC, Loretz CJ, Chang A, Hukriede NA, Bitzer M, Pippin JW, Wessely O, Shankland SJ. Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span. Aging. 2023 July 23;15(14):6658-6689. PubMed PMID:37487005; PubMed Central PMCID: PMC10415579; DOI: 10.18632/aging.204897.
 
Cosgrove HA, Gingras S, Kim M, Bastacky S, Tilstra JS, Shlomchik MJ. B cell-intrinsic TLR7 expression drives severe lupus in TLR9-deficient mice. JCI insight. 2023 August 22;8(16). PubMed PMID: 37606042; PubMed Central PMCID: PMC10543715; DOI:10.1172/jci.insight.172219.
 
DeGiosio RA, Needham PG, Andrews OA, Tristan H, Grubisha MJ, Brodsky JL, Camacho C, Sweet RA. Differential regulation of MAP2 by phosphorylation events in proline-rich versus C terminal domains. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2023 October;37(10):e23194. PubMed PMID: 37702880; PubMed Central PMCID: PMC10539048; DOI: 10.1096/fj.202300486R.
 
Long KR, Rbaibi Y, Kashlan OB, Weisz OA. Receptor-associated protein impairs ligand binding to megalin and megalin-dependent endocytic flux in proximal tubule cells. American journal of physiology. Renal physiology. 2023 October 1;325(4):F457-F464. PubMed PMID:37534387; DOI: 10.1152/ajprenal.00165.2023.
 
Gui Y, Palanza Z, Gupta P, Li H, Pan Y, Wang Y, Hargis G, Kreutzer DL, Wang Y, Bastacky SI, Liu Y, Liu S, Zhou D. Calponin 2 regulates ketogenesis to mitigate acute kidney injury. JCI insight. 2023 November 8;8(21). PubMed PMID: 37751293; PubMed Central PMCID:PMC10721266; DOI: 10.1172/jci.insight.170521.
 
Nickerson AJ, Mutchler SM, Sheng S, Cox NA, Ray EC, Kashlan OB, Carattino MD, Marciszyn AL, Winfrey A, Gingras S, Kirabo A, Hughey RP, Kleyman TR. Mice lacking γENaC palmitoylation sites maintain benzamil-sensitive Na+ transport despite reduced channel activity. JCI insight. 2023 November 8;8(21). PubMed PMID: 37707951; PubMed Central PMCID: PMC10721255; DOI: 10.1172/jci.insight.172051.
 
Apodaca G. Defining the molecular fingerprint of bladder and kidney fibroblasts. American journal of physiology. Renal physiology. 2023 December 1;325(6):F826-F856. PubMed PMID: 37823192; DOI: 10.1152/ajprenal.00284.2023.
 
Montalbetti N, Dalghi MG, Parakala-Jain T, Clayton D, Apodaca G, Carattino MD. Antinociceptive effect of the calcitonin gene-related peptide receptor antagonist BIBN4096BS in mice with bacterial cystitis. American journal of physiology. Renal physiology. 2023 December 1;325(6):F779-F791. PubMed PMID: 37823199; DOI:
10.1152/ajprenal.00217.2023.
 
Tilstra JS, Kim M, Gordon RA, Leibler C, Cosgrove HA, Bastacky S, Nickerson KM, Shlomchik MJ. B cell-intrinsic Myd88 regulates disease progression in murine lupus. The Journal of experimental medicine. 2023 December 4;220(12). PubMed PMID: 37787782; PubMed Central PMCID: PMC10541815; DOI: 10.1084/jem.20230263.
 

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Center Director: Thomas Kleyman, MD

Center Associate Director: Ora Weisz, PhD

Physiology Core Directors: Marcelo Carattino, PhD and Lisa Satlin, MD

Model Systems Core Directors: Arohan Subramanya, MD , Rod Tan, MD, and Jeff Brodsky, PhD

KIDNIT Directors: Gerard Apodaca, PhD and Simon Watkins, PhD

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